commercial ground truth objects for 3D super-resolution microscopy?

[simeng]

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Hi all,

We are developing 3d super-resolution microscopes in 100~150 nm axial resolution range. I had a hard time in finding some 3D commercial fluorescent samples as the ground truth objects for testing our imaging system. Here are some specs of fluorescent samples that we would like to buy:

- Dots/Lines with known separation about 100-200 nm in Z dimension
- Not weak fluorescence since high SNR is required for computing super-resolution

I know Gatta-Quant sell a 3D sample with two dots separated by 90nm in the axial dimension. However, it is a bit too small for us to resolve with the given brightness (10000 photons per dot). Also we do not have the tools/equipment to fold DNA to certain shapes in 3d so we basically rely on commercially prepared samples.

Any idea of where I could buy some bright ground-truth fluorescent samples from?

Thank you very much.

Simeng

[George McNamara]

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http://argolight.com/argo-sim/

or ask Argolight to make structure(s) to your specifications.


On 12/27/2017 6:05 PM, Simeng Chen wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> We are developing 3d super-resolution microscopes in 100~150 nm axial resolution range. I had a hard time in finding some 3D commercial fluorescent samples as the ground truth objects for testing our imaging system. Here are some specs of fluorescent samples that we would like to buy:
>
> - Dots/Lines with known separation about 100-200 nm in Z dimension
> - Not weak fluorescence since high SNR is required for computing super-resolution
>
> I know Gatta-Quant sell a 3D sample with two dots separated by 90nm in the axial dimension. However, it is a bit too small for us to resolve with the given brightness (10000 photons per dot). Also we do not have the tools/equipment to fold DNA to certain shapes in 3d so we basically rely on commercially prepared samples.
>
> Any idea of where I could buy some bright ground-truth fluorescent samples from?
>
> Thank you very much.
>
> Simeng

--


George McNamara, PhD
Baltimore, MD 21231
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75   (may need to use Microsoft Edge or Firefox, rather than Google Chrome)
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
http://confocal.jhu.edu

July 2017 Current Protocols article, open access:
UNIT 4.4 Microscopy and Image Analysis
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/abstract
supporting materials direct link is
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/full#hg0404-sec-0023
figures at
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/figures