confocal PMT intermitently dies

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Arvydas Matiukas Arvydas Matiukas
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confocal PMT intermitently dies

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Dear list,


Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs
of powering on, all image pixels turn to  zero intensity no matter
what are laser power, pinhole, filter, and gain settings are used.
Cold reboot does not fix the problem though no error messages
are reported. The single fix so far is to let the system cool down
for several hours then it is again working for few more hours.
Surprisingly all cooling fans are spinning normally as well as  room
conditioning system.




We are off service contract so any feedback/advice what could
be wrong and how to fix it is greatly appreciated.


Thank you,
Arvydas





Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Imaging Core Facility
Department of Pharmacology
SUNY Upstate Medical University
766 Irving Ave., WH 3159
Syracuse, NY 13210
tel.: 315-464-7997
fax: 315-464-8014
email: [hidden email]
Mark Cannell Mark Cannell
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Re: confocal PMT intermitently dies

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Check connectors...  Unplug and replug all connectors. If that doesn't work then its most likely electronic in the pmt3 power supply or pmt3 preamp stages...

Hope this helps
Mark

On 2/05/2012, at 4:22 PM, Arvydas Matiukas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear list,
>
>
> Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs
> of powering on, all image pixels turn to  zero intensity no matter
> what are laser power, pinhole, filter, and gain settings are used.
> Cold reboot does not fix the problem though no error messages
> are reported. The single fix so far is to let the system cool down
> for several hours then it is again working for few more hours.
> Surprisingly all cooling fans are spinning normally as well as  room
> conditioning system.
>
>
>
>
> We are off service contract so any feedback/advice what could
> be wrong and how to fix it is greatly appreciated.
>
>
> Thank you,
> Arvydas
>
>
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Imaging Core Facility
> Department of Pharmacology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3159
> Syracuse, NY 13210
> tel.: 315-464-7997
> fax: 315-464-8014
> email: [hidden email]
Joseph Bruton Joseph Bruton
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Re: confocal PMT intermitently dies

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Just to add to this, reseat all cards in the computer/controller as well.

Regards,

Joseph Bruton
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Mark Cannell [[hidden email]]
Sent: 02 May 2012 18:21
To: [hidden email]
Subject: Re: confocal PMT intermitently dies

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Check connectors...  Unplug and replug all connectors. If that doesn't work then its most likely electronic in the pmt3 power supply or pmt3 preamp stages...

Hope this helps
Mark

On 2/05/2012, at 4:22 PM, Arvydas Matiukas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear list,
>
>
> Ch3 PMT intermitently dies on our LSM510 system. After 2-4 hrs
> of powering on, all image pixels turn to  zero intensity no matter
> what are laser power, pinhole, filter, and gain settings are used.
> Cold reboot does not fix the problem though no error messages
> are reported. The single fix so far is to let the system cool down
> for several hours then it is again working for few more hours.
> Surprisingly all cooling fans are spinning normally as well as  room
> conditioning system.
>
>
>
>
> We are off service contract so any feedback/advice what could
> be wrong and how to fix it is greatly appreciated.
>
>
> Thank you,
> Arvydas
>
>
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Imaging Core Facility
> Department of Pharmacology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3159
> Syracuse, NY 13210
> tel.: 315-464-7997
> fax: 315-464-8014
> email: [hidden email]