congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry, “ for the development of super-resolved fluores cence microscopy”

[George McNamara]

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*****

congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry

http://www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/press.html


  Press Release

8 October 2014

The Royal Swedish Academy of Sciences
<http://www.nobelprize.org/redirect/links_out/prizeawarder.php?from=/nobel_prizes/chemistry/laureates/2014/press.html&object=kva&to=http://www.kva.se/en/>
has decided to award the Nobel Prize in Chemistry for 2014 to

*Eric Betzig*
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
VA, USA,

*Stefan W. Hell*
Max Planck Institute for Biophysical Chemistry, Göttingen, and German
Cancer Research Center, Heidelberg, Germany

and

*William E. Moerner*
Stanford University, Stanford, CA, USA

/“for the development of super-resolved fluorescence microscopy”/


    Surpassing the limitations of the light microscope

For a long time optical microscopy was held back by a presumed
limitation: that it would never obtain a better resolution than half the
wavelength of light. Helped by fluorescent molecules the Nobel Laureates
in Chemistry 2014 ingeniously circumvented this limitation. Their
ground-breaking work has brought optical microscopy into the nanodimension.

In what has become known as nanoscopy, scientists visualize the pathways
of individual molecules inside living cells. They can see how molecules
create synapses between nerve cells in the brain; they can track
proteins involved in Parkinson’s, Alzheimer’s and Huntington’s diseases
as they aggregate; they follow individual proteins in fertilized eggs as
these divide into embryos.

It was all but obvious that scientists should ever be able to study
living cells in the tiniest molecular detail. In 1873, the microscopist
Ernst Abbe stipulated a physical limit for the maximum resolution of
traditional optical microscopy: it could never become better than 0.2
micrometres. *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner*
are awarded the Nobel Prize in Chemistry 2014 for having bypassed this
limit. Due to their achievements the optical microscope can now peer
into the nanoworld.

Two separate principles are rewarded. One enables the method /stimulated
emission depletion (STED) microscopy/, developed by Stefan Hell in 2000.
Two laser beams are utilized; one stimulates fluorescent molecules to
glow, another cancels out all fluorescence except for that in a
nanometre-sized volume. Scanning over the sample, nanometre for
nanometre, yields an image with a resolution better than Abbe’s
stipulated limit.

Eric Betzig and William Moerner, working separately, laid the foundation
for the second method, /single-molecule microscopy/. The method relies
upon the possibility to turn the fluorescence of individual molecules on
and off. Scientists image the same area multiple times, letting just a
few interspersed molecules glow each time. Superimposing these images
yields a dense super-image resolved at the nanolevel. In 2006 Eric
Betzig utilized this method for the first time.

Today, nanoscopy is used world-wide and new knowledge of greatest
benefit to mankind is produced on a daily basis.


//

My thanks to the Nobel committee for revealing what W.E. is.

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42

[Peng Xi-2]

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*****

Why not Xiaowei Zhuang?


Sincerely,
Peng Xi
Ph. D.    Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: [phone]
Email: [hidden email]
http://bme.pku.edu.cn/~xipeng/

On Wed, Oct 8, 2014 at 7:51 AM, George McNamara <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry
>
> http://www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/press.html
>
>
>  Press Release
>
> 8 October 2014
>
> The Royal Swedish Academy of Sciences <http://www.nobelprize.org/
> redirect/links_out/prizeawarder.php?from=/nobel_
> prizes/chemistry/laureates/2014/press.html&object=kva&to=
> http://www.kva.se/en/> has decided to award the Nobel Prize in Chemistry
> for 2014 to
>
> *Eric Betzig*
> Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
> VA, USA,
>
> *Stefan W. Hell*
> Max Planck Institute for Biophysical Chemistry, Göttingen, and German
> Cancer Research Center, Heidelberg, Germany
>
> and
>
> *William E. Moerner*
> Stanford University, Stanford, CA, USA
>
> /“for the development of super-resolved fluorescence microscopy”/
>
>
>    Surpassing the limitations of the light microscope
>
> For a long time optical microscopy was held back by a presumed limitation:
> that it would never obtain a better resolution than half the wavelength of
> light. Helped by fluorescent molecules the Nobel Laureates in Chemistry
> 2014 ingeniously circumvented this limitation. Their ground-breaking work
> has brought optical microscopy into the nanodimension.
>
> In what has become known as nanoscopy, scientists visualize the pathways
> of individual molecules inside living cells. They can see how molecules
> create synapses between nerve cells in the brain; they can track proteins
> involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they
> aggregate; they follow individual proteins in fertilized eggs as these
> divide into embryos.
>
> It was all but obvious that scientists should ever be able to study living
> cells in the tiniest molecular detail. In 1873, the microscopist Ernst Abbe
> stipulated a physical limit for the maximum resolution of traditional
> optical microscopy: it could never become better than 0.2 micrometres.
> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded the
> Nobel Prize in Chemistry 2014 for having bypassed this limit. Due to their
> achievements the optical microscope can now peer into the nanoworld.
>
> Two separate principles are rewarded. One enables the method /stimulated
> emission depletion (STED) microscopy/, developed by Stefan Hell in 2000.
> Two laser beams are utilized; one stimulates fluorescent molecules to glow,
> another cancels out all fluorescence except for that in a nanometre-sized
> volume. Scanning over the sample, nanometre for nanometre, yields an image
> with a resolution better than Abbe’s stipulated limit.
>
> Eric Betzig and William Moerner, working separately, laid the foundation
> for the second method, /single-molecule microscopy/. The method relies upon
> the possibility to turn the fluorescence of individual molecules on and
> off. Scientists image the same area multiple times, letting just a few
> interspersed molecules glow each time. Superimposing these images yields a
> dense super-image resolved at the nanolevel. In 2006 Eric Betzig utilized
> this method for the first time.
>
> Today, nanoscopy is used world-wide and new knowledge of greatest benefit
> to mankind is produced on a daily basis.
>
>
> //
>
> My thanks to the Nobel committee for revealing what W.E. is.
>
> --
>
>
>
> George McNamara, Ph.D.
> Single Cells Analyst
> L.J.N. Cooper Lab
> University of Texas M.D. Anderson Cancer Center
> Houston, TX 77054
> Tattletales http://works.bepress.com/gmcnamara/42
>

[Martin Wessendorf-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Dr. Xi--

Excellent question, especially since Rust et al 2006 was accepted and
published on-line a day or two prior to Beitzig et al 2006. --Here's the
statement by the chairman of the Nobel committee:
http://www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/announcement.html 
. --According to him, the rationale for the Prize was that STED and PALM
can be used to view biochemistry happening in living organisms.

Do any of our Swedish members have insight into how these decisions are
made?

Martin Wessendorf



On 10/8/2014 7:52 AM, Peng Xi wrote:

> Why not Xiaowei Zhuang?
>
>
> Sincerely,
> Peng Xi
> Ph. D.    Associate Professor
> Dept. of Biomedical Engineering, College of Engineering
> Peking University, Beijing, China
> Tel: [phone]
> Email: [hidden email]
> http://bme.pku.edu.cn/~xipeng/
>
> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry
>>
>> http://www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/press.html
>>
>>
>>   Press Release
>>
>> 8 October 2014
>>
>> The Royal Swedish Academy of Sciences <http://www.nobelprize.org/
>> redirect/links_out/prizeawarder.php?from=/nobel_
>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
>> http://www.kva.se/en/> has decided to award the Nobel Prize in Chemistry
>> for 2014 to
>>
>> *Eric Betzig*
>> Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
>> VA, USA,
>>
>> *Stefan W. Hell*
>> Max Planck Institute for Biophysical Chemistry, Göttingen, and German
>> Cancer Research Center, Heidelberg, Germany
>>
>> and
>>
>> *William E. Moerner*
>> Stanford University, Stanford, CA, USA
>>
>> /“for the development of super-resolved fluorescence microscopy”/
>>
>>
>>     Surpassing the limitations of the light microscope
>>
>> For a long time optical microscopy was held back by a presumed limitation:
>> that it would never obtain a better resolution than half the wavelength of
>> light. Helped by fluorescent molecules the Nobel Laureates in Chemistry
>> 2014 ingeniously circumvented this limitation. Their ground-breaking work
>> has brought optical microscopy into the nanodimension.
>>
>> In what has become known as nanoscopy, scientists visualize the pathways
>> of individual molecules inside living cells. They can see how molecules
>> create synapses between nerve cells in the brain; they can track proteins
>> involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they
>> aggregate; they follow individual proteins in fertilized eggs as these
>> divide into embryos.
>>
>> It was all but obvious that scientists should ever be able to study living
>> cells in the tiniest molecular detail. In 1873, the microscopist Ernst Abbe
>> stipulated a physical limit for the maximum resolution of traditional
>> optical microscopy: it could never become better than 0.2 micrometres.
>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded the
>> Nobel Prize in Chemistry 2014 for having bypassed this limit. Due to their
>> achievements the optical microscope can now peer into the nanoworld.
>>
>> Two separate principles are rewarded. One enables the method /stimulated
>> emission depletion (STED) microscopy/, developed by Stefan Hell in 2000.
>> Two laser beams are utilized; one stimulates fluorescent molecules to glow,
>> another cancels out all fluorescence except for that in a nanometre-sized
>> volume. Scanning over the sample, nanometre for nanometre, yields an image
>> with a resolution better than Abbe’s stipulated limit.
>>
>> Eric Betzig and William Moerner, working separately, laid the foundation
>> for the second method, /single-molecule microscopy/. The method relies upon
>> the possibility to turn the fluorescence of individual molecules on and
>> off. Scientists image the same area multiple times, letting just a few
>> interspersed molecules glow each time. Superimposing these images yields a
>> dense super-image resolved at the nanolevel. In 2006 Eric Betzig utilized
>> this method for the first time.
>>
>> Today, nanoscopy is used world-wide and new knowledge of greatest benefit
>> to mankind is produced on a daily basis.
>>
>>
>> //
>>
>> My thanks to the Nobel committee for revealing what W.E. is.
>>
>> --
>>
>>
>>
>> George McNamara, Ph.D.
>> Single Cells Analyst
>> L.J.N. Cooper Lab
>> University of Texas M.D. Anderson Cancer Center
>> Houston, TX 77054
>> Tattletales http://works.bepress.com/gmcnamara/42
>>

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Daniel Sjolander]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

"Do any of our Swedish members have insight into how these decisions are
made?"

Behind closed doors ;)

Best,
Daniel Sjölander

On Wed, Oct 8, 2014 at 4:10 PM, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Dr. Xi--
>
> Excellent question, especially since Rust et al 2006 was accepted and
> published on-line a day or two prior to Beitzig et al 2006. --Here's the
> statement by the chairman of the Nobel committee:
> http://www.nobelprize.org/nobel_prizes/chemistry/
> laureates/2014/announcement.html . --According to him, the rationale for
> the Prize was that STED and PALM can be used to view biochemistry happening
> in living organisms.
>
> Do any of our Swedish members have insight into how these decisions are
> made?
>
> Martin Wessendorf
>
>
>
>
> On 10/8/2014 7:52 AM, Peng Xi wrote:
>
>> Why not Xiaowei Zhuang?
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D.    Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering
>> Peking University, Beijing, China
>> Tel: [phone]
>> Email: [hidden email]
>> http://bme.pku.edu.cn/~xipeng/
>>
>> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara <
>> [hidden email]>
>> wrote:
>>
>>  *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>>> posting.
>>> *****
>>>
>>> congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry
>>>
>>> http://www.nobelprize.org/nobel_prizes/chemistry/
>>> laureates/2014/press.html
>>>
>>>
>>>   Press Release
>>>
>>> 8 October 2014
>>>
>>> The Royal Swedish Academy of Sciences <http://www.nobelprize.org/
>>> redirect/links_out/prizeawarder.php?from=/nobel_
>>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
>>> http://www.kva.se/en/> has decided to award the Nobel Prize in Chemistry
>>> for 2014 to
>>>
>>> *Eric Betzig*
>>> Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
>>> VA, USA,
>>>
>>> *Stefan W. Hell*
>>> Max Planck Institute for Biophysical Chemistry, Göttingen, and German
>>> Cancer Research Center, Heidelberg, Germany
>>>
>>> and
>>>
>>> *William E. Moerner*
>>> Stanford University, Stanford, CA, USA
>>>
>>> /“for the development of super-resolved fluorescence microscopy”/
>>>
>>>
>>>     Surpassing the limitations of the light microscope
>>>
>>> For a long time optical microscopy was held back by a presumed
>>> limitation:
>>> that it would never obtain a better resolution than half the wavelength
>>> of
>>> light. Helped by fluorescent molecules the Nobel Laureates in Chemistry
>>> 2014 ingeniously circumvented this limitation. Their ground-breaking work
>>> has brought optical microscopy into the nanodimension.
>>>
>>> In what has become known as nanoscopy, scientists visualize the pathways
>>> of individual molecules inside living cells. They can see how molecules
>>> create synapses between nerve cells in the brain; they can track proteins
>>> involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they
>>> aggregate; they follow individual proteins in fertilized eggs as these
>>> divide into embryos.
>>>
>>> It was all but obvious that scientists should ever be able to study
>>> living
>>> cells in the tiniest molecular detail. In 1873, the microscopist Ernst
>>> Abbe
>>> stipulated a physical limit for the maximum resolution of traditional
>>> optical microscopy: it could never become better than 0.2 micrometres.
>>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded the
>>> Nobel Prize in Chemistry 2014 for having bypassed this limit. Due to
>>> their
>>> achievements the optical microscope can now peer into the nanoworld.
>>>
>>> Two separate principles are rewarded. One enables the method /stimulated
>>> emission depletion (STED) microscopy/, developed by Stefan Hell in 2000.
>>> Two laser beams are utilized; one stimulates fluorescent molecules to
>>> glow,
>>> another cancels out all fluorescence except for that in a nanometre-sized
>>> volume. Scanning over the sample, nanometre for nanometre, yields an
>>> image
>>> with a resolution better than Abbe’s stipulated limit.
>>>
>>> Eric Betzig and William Moerner, working separately, laid the foundation
>>> for the second method, /single-molecule microscopy/. The method relies
>>> upon
>>> the possibility to turn the fluorescence of individual molecules on and
>>> off. Scientists image the same area multiple times, letting just a few
>>> interspersed molecules glow each time. Superimposing these images yields
>>> a
>>> dense super-image resolved at the nanolevel. In 2006 Eric Betzig utilized
>>> this method for the first time.
>>>
>>> Today, nanoscopy is used world-wide and new knowledge of greatest benefit
>>> to mankind is produced on a daily basis.
>>>
>>>
>>> //
>>>
>>> My thanks to the Nobel committee for revealing what W.E. is.
>>>
>>> --
>>>
>>>
>>>
>>> George McNamara, Ph.D.
>>> Single Cells Analyst
>>> L.J.N. Cooper Lab
>>> University of Texas M.D. Anderson Cancer Center
>>> Houston, TX 77054
>>> Tattletales http://works.bepress.com/gmcnamara/42
>>>
>>>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

[Feinstein, Timothy]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,

Hm.  The Betzig group went live cell first in Nature Methods 5:155 and
5:417 (2008), but the Zhuang group did compelling work in PNAS 109:13978
(2012) that also broke ground in live imaging involving localization
microscopy.  I think the three name limit put the Committee in a very hard
spot here.  

Best,


Tim

Timothy Feinstein, Ph.D. | Confocal Manager
333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
Phone: [phone] | Email: [hidden email]







On 10/8/14, 10:10 AM, "Martin Wessendorf" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_eez
>PeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocalmic
>roscopy
>Post images on
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_ebn
>OK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
>posting.
>*****
>
>Dear Dr. Xi--
>
>Excellent question, especially since Rust et al 2006 was accepted and
>published on-line a day or two prior to Beitzig et al 2006. --Here's the
>statement by the chairman of the Nobel committee:
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_eej
>BflCROA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fchemistry
>%2flaureates%2f2014%2fannouncement%2ehtml
>. --According to him, the rationale for the Prize was that STED and PALM
>can be used to view biochemistry happening in living organisms.
>
>Do any of our Swedish members have insight into how these decisions are
>made?
>
>Martin Wessendorf
>
>
>
>On 10/8/2014 7:52 AM, Peng Xi wrote:
>> Why not Xiaowei Zhuang?
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D.    Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering
>> Peking University, Beijing, China
>> Tel: [phone]
>> Email: [hidden email]
>>
>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_eb
>>rBKV7FMA&u=http%3a%2f%2fbme%2epku%2eedu%2ecn%2f%7exipeng%2f
>>
>> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara
>><[hidden email]>
>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_e
>>>ezPeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfoca
>>>lmicroscopy
>>> Post images on
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_e
>>>bnOK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in your
>>>posting.
>>> *****
>>>
>>> congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry
>>>
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_e
>>>b7KIwPFOA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fchemi
>>>stry%2flaureates%2f2014%2fpress%2ehtml
>>>
>>>
>>>   Press Release
>>>
>>> 8 October 2014
>>>
>>> The Royal Swedish Academy of Sciences
>>><http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>>>eb3LfQSWaQ&u=http%3a%2f%2fwww%2enobelprize%2eorg%2f
>>> redirect/links_out/prizeawarder.php?from=/nobel_
>>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_e
>>>b6dflWUbg&u=http%3a%2f%2fwww%2ekva%2ese%2fen%2f> has decided to award
>>>the Nobel Prize in Chemistry
>>> for 2014 to
>>>
>>> *Eric Betzig*
>>> Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
>>> VA, USA,
>>>
>>> *Stefan W. Hell*
>>> Max Planck Institute for Biophysical Chemistry, Göttingen, and German
>>> Cancer Research Center, Heidelberg, Germany
>>>
>>> and
>>>
>>> *William E. Moerner*
>>> Stanford University, Stanford, CA, USA
>>>
>>> /³for the development of super-resolved fluorescence microscopy²/
>>>
>>>
>>>     Surpassing the limitations of the light microscope
>>>
>>> For a long time optical microscopy was held back by a presumed
>>>limitation:
>>> that it would never obtain a better resolution than half the
>>>wavelength of
>>> light. Helped by fluorescent molecules the Nobel Laureates in Chemistry
>>> 2014 ingeniously circumvented this limitation. Their ground-breaking
>>>work
>>> has brought optical microscopy into the nanodimension.
>>>
>>> In what has become known as nanoscopy, scientists visualize the
>>>pathways
>>> of individual molecules inside living cells. They can see how molecules
>>> create synapses between nerve cells in the brain; they can track
>>>proteins
>>> involved in Parkinson¹s, Alzheimer¹s and Huntington¹s diseases as they
>>> aggregate; they follow individual proteins in fertilized eggs as these
>>> divide into embryos.
>>>
>>> It was all but obvious that scientists should ever be able to study
>>>living
>>> cells in the tiniest molecular detail. In 1873, the microscopist Ernst
>>>Abbe
>>> stipulated a physical limit for the maximum resolution of traditional
>>> optical microscopy: it could never become better than 0.2 micrometres.
>>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded
>>>the
>>> Nobel Prize in Chemistry 2014 for having bypassed this limit. Due to
>>>their
>>> achievements the optical microscope can now peer into the nanoworld.
>>>
>>> Two separate principles are rewarded. One enables the method
>>>/stimulated
>>> emission depletion (STED) microscopy/, developed by Stefan Hell in
>>>2000.
>>> Two laser beams are utilized; one stimulates fluorescent molecules to
>>>glow,
>>> another cancels out all fluorescence except for that in a
>>>nanometre-sized
>>> volume. Scanning over the sample, nanometre for nanometre, yields an
>>>image
>>> with a resolution better than Abbe¹s stipulated limit.
>>>
>>> Eric Betzig and William Moerner, working separately, laid the
>>>foundation
>>> for the second method, /single-molecule microscopy/. The method relies
>>>upon
>>> the possibility to turn the fluorescence of individual molecules on and
>>> off. Scientists image the same area multiple times, letting just a few
>>> interspersed molecules glow each time. Superimposing these images
>>>yields a
>>> dense super-image resolved at the nanolevel. In 2006 Eric Betzig
>>>utilized
>>> this method for the first time.
>>>
>>> Today, nanoscopy is used world-wide and new knowledge of greatest
>>>benefit
>>> to mankind is produced on a daily basis.
>>>
>>>
>>> //
>>>
>>> My thanks to the Nobel committee for revealing what W.E. is.
>>>
>>> --
>>>
>>>
>>>
>>> George McNamara, Ph.D.
>>> Single Cells Analyst
>>> L.J.N. Cooper Lab
>>> University of Texas M.D. Anderson Cancer Center
>>> Houston, TX 77054
>>> Tattletales
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_e
>>>ePLeAaSbQ&u=http%3a%2f%2fworks%2ebepress%2ecom%2fgmcnamara%2f42
>>>
>
>--
>Martin Wessendorf, Ph.D.                   office: [phone]
>Assoc Prof, Dept Neuroscience                 lab: [phone]
>University of Minnesota             Preferred FAX: [phone]
>6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>Minneapolis, MN  55455                    e-mail: [hidden email]

[Rosemary.White]

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*****

Yes, indeed! Fantastic!

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of George McNamara [[hidden email]]
Sent: Wednesday, 8 October 2014 10:51 p.m.
To: [hidden email]
Subject: congratulations to Eric, Stefan and  W.E. for Nobel Prize in Chemistry, “ for the development of super-resolved fluores cence microscopy”

*****
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*****

congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry

http://www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/press.html


  Press Release

8 October 2014

The Royal Swedish Academy of Sciences
<http://www.nobelprize.org/redirect/links_out/prizeawarder.php?from=/nobel_prizes/chemistry/laureates/2014/press.html&object=kva&to=http://www.kva.se/en/>
has decided to award the Nobel Prize in Chemistry for 2014 to

*Eric Betzig*
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn,
VA, USA,

*Stefan W. Hell*
Max Planck Institute for Biophysical Chemistry, Göttingen, and German
Cancer Research Center, Heidelberg, Germany

and

*William E. Moerner*
Stanford University, Stanford, CA, USA

/“for the development of super-resolved fluorescence microscopy”/


    Surpassing the limitations of the light microscope

For a long time optical microscopy was held back by a presumed
limitation: that it would never obtain a better resolution than half the
wavelength of light. Helped by fluorescent molecules the Nobel Laureates
in Chemistry 2014 ingeniously circumvented this limitation. Their
ground-breaking work has brought optical microscopy into the nanodimension.

In what has become known as nanoscopy, scientists visualize the pathways
of individual molecules inside living cells. They can see how molecules
create synapses between nerve cells in the brain; they can track
proteins involved in Parkinson’s, Alzheimer’s and Huntington’s diseases
as they aggregate; they follow individual proteins in fertilized eggs as
these divide into embryos.

It was all but obvious that scientists should ever be able to study
living cells in the tiniest molecular detail. In 1873, the microscopist
Ernst Abbe stipulated a physical limit for the maximum resolution of
traditional optical microscopy: it could never become better than 0.2
micrometres. *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner*
are awarded the Nobel Prize in Chemistry 2014 for having bypassed this
limit. Due to their achievements the optical microscope can now peer
into the nanoworld.

Two separate principles are rewarded. One enables the method /stimulated
emission depletion (STED) microscopy/, developed by Stefan Hell in 2000.
Two laser beams are utilized; one stimulates fluorescent molecules to
glow, another cancels out all fluorescence except for that in a
nanometre-sized volume. Scanning over the sample, nanometre for
nanometre, yields an image with a resolution better than Abbe’s
stipulated limit.

Eric Betzig and William Moerner, working separately, laid the foundation
for the second method, /single-molecule microscopy/. The method relies
upon the possibility to turn the fluorescence of individual molecules on
and off. Scientists image the same area multiple times, letting just a
few interspersed molecules glow each time. Superimposing these images
yields a dense super-image resolved at the nanolevel. In 2006 Eric
Betzig utilized this method for the first time.

Today, nanoscopy is used world-wide and new knowledge of greatest
benefit to mankind is produced on a daily basis.


//

My thanks to the Nobel committee for revealing what W.E. is.

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42

[Guy Cox-2]

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*****

The three names is always a problem - especially when the prize is awarded essentially for two different techniques.  Christoph Cremer (Stefan's supervisor) could well also feel he'd been passed over.

                                                            Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Feinstein, Timothy
Sent: Thursday, 9 October 2014 1:43 AM
To: [hidden email]
Subject: Re: congratulations to Eric, Stefan and W .E. for Nob el P rize in Chemistry, ³ for the development of super-reso lved fluores cence mi croscopy²

*****
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*****

Hi all,

Hm.  The Betzig group went live cell first in Nature Methods 5:155 and
5:417 (2008), but the Zhuang group did compelling work in PNAS 109:13978
(2012) that also broke ground in live imaging involving localization microscopy.  I think the three name limit put the Committee in a very hard spot here.  

Best,


Tim

Timothy Feinstein, Ph.D. | Confocal Manager
333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
Phone: [phone] | Email: [hidden email]







On 10/8/14, 10:10 AM, "Martin Wessendorf" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>eez
>PeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocal
>mic
>roscopy
>Post images on
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>ebn OK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in
>your posting.
>*****
>
>Dear Dr. Xi--
>
>Excellent question, especially since Rust et al 2006 was accepted and
>published on-line a day or two prior to Beitzig et al 2006. --Here's
>the statement by the chairman of the Nobel committee:
>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>eej
>BflCROA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fchemis
>try %2flaureates%2f2014%2fannouncement%2ehtml
>. --According to him, the rationale for the Prize was that STED and
>PALM can be used to view biochemistry happening in living organisms.
>
>Do any of our Swedish members have insight into how these decisions are
>made?
>
>Martin Wessendorf
>
>
>
>On 10/8/2014 7:52 AM, Peng Xi wrote:
>> Why not Xiaowei Zhuang?
>>
>>
>> Sincerely,
>> Peng Xi
>> Ph. D.    Associate Professor
>> Dept. of Biomedical Engineering, College of Engineering Peking
>> University, Beijing, China
>> Tel: [phone]
>> Email: [hidden email]
>>
>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN
>>_eb rBKV7FMA&u=http%3a%2f%2fbme%2epku%2eedu%2ecn%2f%7exipeng%2f
>>
>> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara
>><[hidden email]>
>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>N_e
>>>ezPeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconf
>>>oca
>>>lmicroscopy
>>> Post images on
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>N_e bnOK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link
>>>in your posting.
>>> *****
>>>
>>> congratulations to Eric, Stefan and W.E. for Nobel Prize in
>>> Chemistry
>>>
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>N_e
>>>b7KIwPFOA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fch
>>>emi stry%2flaureates%2f2014%2fpress%2ehtml
>>>
>>>
>>>   Press Release
>>>
>>> 8 October 2014
>>>
>>> The Royal Swedish Academy of Sciences
>>><http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93p
>>>RN_ eb3LfQSWaQ&u=http%3a%2f%2fwww%2enobelprize%2eorg%2f
>>> redirect/links_out/prizeawarder.php?from=/nobel_
>>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
>>>
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>N_e b6dflWUbg&u=http%3a%2f%2fwww%2ekva%2ese%2fen%2f> has decided to
>>>award the Nobel Prize in Chemistry  for 2014 to
>>>
>>> *Eric Betzig*
>>> Janelia Farm Research Campus, Howard Hughes Medical Institute,
>>> Ashburn, VA, USA,
>>>
>>> *Stefan W. Hell*
>>> Max Planck Institute for Biophysical Chemistry, Göttingen, and
>>> German Cancer Research Center, Heidelberg, Germany
>>>
>>> and
>>>
>>> *William E. Moerner*
>>> Stanford University, Stanford, CA, USA
>>>
>>> /³for the development of super-resolved fluorescence microscopy²/
>>>
>>>
>>>     Surpassing the limitations of the light microscope
>>>
>>> For a long time optical microscopy was held back by a presumed
>>>limitation:
>>> that it would never obtain a better resolution than half the
>>>wavelength of  light. Helped by fluorescent molecules the Nobel
>>>Laureates in Chemistry
>>> 2014 ingeniously circumvented this limitation. Their ground-breaking
>>>work  has brought optical microscopy into the nanodimension.
>>>
>>> In what has become known as nanoscopy, scientists visualize the
>>>pathways  of individual molecules inside living cells. They can see
>>>how molecules  create synapses between nerve cells in the brain; they
>>>can track proteins  involved in Parkinson¹s, Alzheimer¹s and
>>>Huntington¹s diseases as they  aggregate; they follow individual
>>>proteins in fertilized eggs as these  divide into embryos.
>>>
>>> It was all but obvious that scientists should ever be able to study
>>>living  cells in the tiniest molecular detail. In 1873, the
>>>microscopist Ernst Abbe  stipulated a physical limit for the maximum
>>>resolution of traditional  optical microscopy: it could never become
>>>better than 0.2 micrometres.
>>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded
>>>the  Nobel Prize in Chemistry 2014 for having bypassed this limit.
>>>Due to their  achievements the optical microscope can now peer into
>>>the nanoworld.
>>>
>>> Two separate principles are rewarded. One enables the method
>>>/stimulated  emission depletion (STED) microscopy/, developed by
>>>Stefan Hell in 2000.
>>> Two laser beams are utilized; one stimulates fluorescent molecules
>>>to glow,  another cancels out all fluorescence except for that in a
>>>nanometre-sized  volume. Scanning over the sample, nanometre for
>>>nanometre, yields an image  with a resolution better than Abbe¹s
>>>stipulated limit.
>>>
>>> Eric Betzig and William Moerner, working separately, laid the
>>>foundation  for the second method, /single-molecule microscopy/. The
>>>method relies upon  the possibility to turn the fluorescence of
>>>individual molecules on and  off. Scientists image the same area
>>>multiple times, letting just a few  interspersed molecules glow each
>>>time. Superimposing these images yields a  dense super-image resolved
>>>at the nanolevel. In 2006 Eric Betzig utilized  this method for the
>>>first time.
>>>
>>> Today, nanoscopy is used world-wide and new knowledge of greatest
>>>benefit  to mankind is produced on a daily basis.
>>>
>>>
>>> //
>>>
>>> My thanks to the Nobel committee for revealing what W.E. is.
>>>
>>> --
>>>
>>>
>>>
>>> George McNamara, Ph.D.
>>> Single Cells Analyst
>>> L.J.N. Cooper Lab
>>> University of Texas M.D. Anderson Cancer Center  Houston, TX 77054  
>>>Tattletales
>>>http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>N_e
>>>ePLeAaSbQ&u=http%3a%2f%2fworks%2ebepress%2ecom%2fgmcnamara%2f42
>>>
>
>--
>Martin Wessendorf, Ph.D.                   office: [phone]
>Assoc Prof, Dept Neuroscience                 lab: [phone]
>University of Minnesota             Preferred FAX: [phone]
>6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>Minneapolis, MN  55455                    e-mail: [hidden email]

[Nuno Moreno]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Guy

You're absolutely right. I actually sent an internal email (for the institute) a few hours after the announcement with, among other stuff, this:

"As always there are many others that should be on the laureates list but one needed the final click. In my opinion Cremer from Heidelberg university should be one of them ...."

Nuno Moreno





On 09 Oct 2014, at 02:16, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> The three names is always a problem - especially when the prize is awarded essentially for two different techniques.  Christoph Cremer (Stefan's supervisor) could well also feel he'd been passed over.
>
>                                                            Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Feinstein, Timothy
> Sent: Thursday, 9 October 2014 1:43 AM
> To: [hidden email]
> Subject: Re: congratulations to Eric, Stefan and W .E. for Nob el P rize in Chemistry, ³ for the development of super-reso lved fluores cence mi croscopy²
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi all,
>
> Hm.  The Betzig group went live cell first in Nature Methods 5:155 and
> 5:417 (2008), but the Zhuang group did compelling work in PNAS 109:13978
> (2012) that also broke ground in live imaging involving localization microscopy.  I think the three name limit put the Committee in a very hard spot here.  
>
> Best,
>
>
> Tim
>
> Timothy Feinstein, Ph.D. | Confocal Manager
> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> Phone: [phone] | Email: [hidden email]
>
>
>
>
>
>
>
> On 10/8/14, 10:10 AM, "Martin Wessendorf" <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>> eez
>> PeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocal
>> mic
>> roscopy
>> Post images on
>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>> ebn OK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in
>> your posting.
>> *****
>>
>> Dear Dr. Xi--
>>
>> Excellent question, especially since Rust et al 2006 was accepted and
>> published on-line a day or two prior to Beitzig et al 2006. --Here's
>> the statement by the chairman of the Nobel committee:
>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
>> eej
>> BflCROA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fchemis
>> try %2flaureates%2f2014%2fannouncement%2ehtml
>> . --According to him, the rationale for the Prize was that STED and
>> PALM can be used to view biochemistry happening in living organisms.
>>
>> Do any of our Swedish members have insight into how these decisions are
>> made?
>>
>> Martin Wessendorf
>>
>>
>>
>> On 10/8/2014 7:52 AM, Peng Xi wrote:
>>> Why not Xiaowei Zhuang?
>>>
>>>
>>> Sincerely,
>>> Peng Xi
>>> Ph. D.    Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering Peking
>>> University, Beijing, China
>>> Tel: [phone]
>>> Email: [hidden email]
>>>
>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN
>>> _eb rBKV7FMA&u=http%3a%2f%2fbme%2epku%2eedu%2ecn%2f%7exipeng%2f
>>>
>>> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara
>>> <[hidden email]>
>>> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>
>>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>> N_e
>>>> ezPeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconf
>>>> oca
>>>> lmicroscopy
>>>> Post images on
>>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>> N_e bnOK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link
>>>> in your posting.
>>>> *****
>>>>
>>>> congratulations to Eric, Stefan and W.E. for Nobel Prize in
>>>> Chemistry
>>>>
>>>>
>>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>> N_e
>>>> b7KIwPFOA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fch
>>>> emi stry%2flaureates%2f2014%2fpress%2ehtml
>>>>
>>>>
>>>>  Press Release
>>>>
>>>> 8 October 2014
>>>>
>>>> The Royal Swedish Academy of Sciences
>>>> <http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93p
>>>> RN_ eb3LfQSWaQ&u=http%3a%2f%2fwww%2enobelprize%2eorg%2f
>>>> redirect/links_out/prizeawarder.php?from=/nobel_
>>>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
>>>>
>>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>> N_e b6dflWUbg&u=http%3a%2f%2fwww%2ekva%2ese%2fen%2f> has decided to
>>>> award the Nobel Prize in Chemistry  for 2014 to
>>>>
>>>> *Eric Betzig*
>>>> Janelia Farm Research Campus, Howard Hughes Medical Institute,
>>>> Ashburn, VA, USA,
>>>>
>>>> *Stefan W. Hell*
>>>> Max Planck Institute for Biophysical Chemistry, Göttingen, and
>>>> German Cancer Research Center, Heidelberg, Germany
>>>>
>>>> and
>>>>
>>>> *William E. Moerner*
>>>> Stanford University, Stanford, CA, USA
>>>>
>>>> /³for the development of super-resolved fluorescence microscopy²/
>>>>
>>>>
>>>>    Surpassing the limitations of the light microscope
>>>>
>>>> For a long time optical microscopy was held back by a presumed
>>>> limitation:
>>>> that it would never obtain a better resolution than half the
>>>> wavelength of  light. Helped by fluorescent molecules the Nobel
>>>> Laureates in Chemistry
>>>> 2014 ingeniously circumvented this limitation. Their ground-breaking
>>>> work  has brought optical microscopy into the nanodimension.
>>>>
>>>> In what has become known as nanoscopy, scientists visualize the
>>>> pathways  of individual molecules inside living cells. They can see
>>>> how molecules  create synapses between nerve cells in the brain; they
>>>> can track proteins  involved in Parkinson¹s, Alzheimer¹s and
>>>> Huntington¹s diseases as they  aggregate; they follow individual
>>>> proteins in fertilized eggs as these  divide into embryos.
>>>>
>>>> It was all but obvious that scientists should ever be able to study
>>>> living  cells in the tiniest molecular detail. In 1873, the
>>>> microscopist Ernst Abbe  stipulated a physical limit for the maximum
>>>> resolution of traditional  optical microscopy: it could never become
>>>> better than 0.2 micrometres.
>>>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded
>>>> the  Nobel Prize in Chemistry 2014 for having bypassed this limit.
>>>> Due to their  achievements the optical microscope can now peer into
>>>> the nanoworld.
>>>>
>>>> Two separate principles are rewarded. One enables the method
>>>> /stimulated  emission depletion (STED) microscopy/, developed by
>>>> Stefan Hell in 2000.
>>>> Two laser beams are utilized; one stimulates fluorescent molecules
>>>> to glow,  another cancels out all fluorescence except for that in a
>>>> nanometre-sized  volume. Scanning over the sample, nanometre for
>>>> nanometre, yields an image  with a resolution better than Abbe¹s
>>>> stipulated limit.
>>>>
>>>> Eric Betzig and William Moerner, working separately, laid the
>>>> foundation  for the second method, /single-molecule microscopy/. The
>>>> method relies upon  the possibility to turn the fluorescence of
>>>> individual molecules on and  off. Scientists image the same area
>>>> multiple times, letting just a few  interspersed molecules glow each
>>>> time. Superimposing these images yields a  dense super-image resolved
>>>> at the nanolevel. In 2006 Eric Betzig utilized  this method for the
>>>> first time.
>>>>
>>>> Today, nanoscopy is used world-wide and new knowledge of greatest
>>>> benefit  to mankind is produced on a daily basis.
>>>>
>>>>
>>>> //
>>>>
>>>> My thanks to the Nobel committee for revealing what W.E. is.
>>>>
>>>> --
>>>>
>>>>
>>>>
>>>> George McNamara, Ph.D.
>>>> Single Cells Analyst
>>>> L.J.N. Cooper Lab
>>>> University of Texas M.D. Anderson Cancer Center  Houston, TX 77054  
>>>> Tattletales
>>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
>>>> N_e
>>>> ePLeAaSbQ&u=http%3a%2f%2fworks%2ebepress%2ecom%2fgmcnamara%2f42
>>>>
>>
>> --
>> Martin Wessendorf, Ph.D.                   office: [phone]
>> Assoc Prof, Dept Neuroscience                 lab: [phone]
>> University of Minnesota             Preferred FAX: [phone]
>> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>> Minneapolis, MN  55455                    e-mail: [hidden email]

[Lu Yan]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Maybe splitting it into two halves, one to STED, another one to single
molecule so that more people can be on the list.

-----------------------------------------------------
Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
8 St. Mary St., Boston, MA, 02215
[phone]
[hidden email]
-----------------------------------------------------

On Thu, Oct 9, 2014 at 6:18 PM, Nuno Moreno <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Guy
>
> You're absolutely right. I actually sent an internal email (for the
> institute) a few hours after the announcement with, among other stuff, this:
>
> "As always there are many others that should be on the laureates list but
> one needed the final click. In my opinion Cremer from Heidelberg university
> should be one of them ...."
>
> Nuno Moreno
>
>
>
>
>
> On 09 Oct 2014, at 02:16, Guy Cox <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > The three names is always a problem - especially when the prize is
> awarded essentially for two different techniques.  Christoph Cremer
> (Stefan's supervisor) could well also feel he'd been passed over.
> >
> >                                                            Guy
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Feinstein, Timothy
> > Sent: Thursday, 9 October 2014 1:43 AM
> > To: [hidden email]
> > Subject: Re: congratulations to Eric, Stefan and W .E. for Nob el P rize
> in Chemistry, ³ for the development of super-reso lved fluores cence mi
> croscopy²
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi all,
> >
> > Hm.  The Betzig group went live cell first in Nature Methods 5:155 and
> > 5:417 (2008), but the Zhuang group did compelling work in PNAS 109:13978
> > (2012) that also broke ground in live imaging involving localization
> microscopy.  I think the three name limit put the Committee in a very hard
> spot here.
> >
> > Best,
> >
> >
> > Tim
> >
> > Timothy Feinstein, Ph.D. | Confocal Manager
> > 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> > Phone: [phone] | Email: [hidden email]
> >
> >
> >
> >
> >
> >
> >
> > On 10/8/14, 10:10 AM, "Martin Wessendorf" <[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
> >> eez
> >> PeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconfocal
> >> mic
> >> roscopy
> >> Post images on
> >> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
> >> ebn OK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in
> >> your posting.
> >> *****
> >>
> >> Dear Dr. Xi--
> >>
> >> Excellent question, especially since Rust et al 2006 was accepted and
> >> published on-line a day or two prior to Beitzig et al 2006. --Here's
> >> the statement by the chairman of the Nobel committee:
> >> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN_
> >> eej
> >> BflCROA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fchemis
> >> try %2flaureates%2f2014%2fannouncement%2ehtml
> >> . --According to him, the rationale for the Prize was that STED and
> >> PALM can be used to view biochemistry happening in living organisms.
> >>
> >> Do any of our Swedish members have insight into how these decisions are
> >> made?
> >>
> >> Martin Wessendorf
> >>
> >>
> >>
> >> On 10/8/2014 7:52 AM, Peng Xi wrote:
> >>> Why not Xiaowei Zhuang?
> >>>
> >>>
> >>> Sincerely,
> >>> Peng Xi
> >>> Ph. D.    Associate Professor
> >>> Dept. of Biomedical Engineering, College of Engineering Peking
> >>> University, Beijing, China
> >>> Tel: [phone]
> >>> Email: [hidden email]
> >>>
> >>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pRN
> >>> _eb rBKV7FMA&u=http%3a%2f%2fbme%2epku%2eedu%2ecn%2f%7exipeng%2f
> >>>
> >>> On Wed, Oct 8, 2014 at 7:51 AM, George McNamara
> >>> <[hidden email]>
> >>> wrote:
> >>>
> >>>> *****
> >>>> To join, leave or search the confocal microscopy listserv, go to:
> >>>>
> >>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
> >>>> N_e
> >>>> ezPeV-TaQ&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-bin%2fwa%3fA0%3dconf
> >>>> oca
> >>>> lmicroscopy
> >>>> Post images on
> >>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
> >>>> N_e bnOK1XHOQ&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link
> >>>> in your posting.
> >>>> *****
> >>>>
> >>>> congratulations to Eric, Stefan and W.E. for Nobel Prize in
> >>>> Chemistry
> >>>>
> >>>>
> >>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
> >>>> N_e
> >>>> b7KIwPFOA&u=http%3a%2f%2fwww%2enobelprize%2eorg%2fnobel%5fprizes%2fch
> >>>> emi stry%2flaureates%2f2014%2fpress%2ehtml
> >>>>
> >>>>
> >>>>  Press Release
> >>>>
> >>>> 8 October 2014
> >>>>
> >>>> The Royal Swedish Academy of Sciences
> >>>> <http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93p
> >>>> RN_ eb3LfQSWaQ&u=http%3a%2f%2fwww%2enobelprize%2eorg%2f
> >>>> redirect/links_out/prizeawarder.php?from=/nobel_
> >>>> prizes/chemistry/laureates/2014/press.html&object=kva&to=
> >>>>
> >>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
> >>>> N_e b6dflWUbg&u=http%3a%2f%2fwww%2ekva%2ese%2fen%2f> has decided to
> >>>> award the Nobel Prize in Chemistry  for 2014 to
> >>>>
> >>>> *Eric Betzig*
> >>>> Janelia Farm Research Campus, Howard Hughes Medical Institute,
> >>>> Ashburn, VA, USA,
> >>>>
> >>>> *Stefan W. Hell*
> >>>> Max Planck Institute for Biophysical Chemistry, Göttingen, and
> >>>> German Cancer Research Center, Heidelberg, Germany
> >>>>
> >>>> and
> >>>>
> >>>> *William E. Moerner*
> >>>> Stanford University, Stanford, CA, USA
> >>>>
> >>>> /³for the development of super-resolved fluorescence microscopy²/
> >>>>
> >>>>
> >>>>    Surpassing the limitations of the light microscope
> >>>>
> >>>> For a long time optical microscopy was held back by a presumed
> >>>> limitation:
> >>>> that it would never obtain a better resolution than half the
> >>>> wavelength of  light. Helped by fluorescent molecules the Nobel
> >>>> Laureates in Chemistry
> >>>> 2014 ingeniously circumvented this limitation. Their ground-breaking
> >>>> work  has brought optical microscopy into the nanodimension.
> >>>>
> >>>> In what has become known as nanoscopy, scientists visualize the
> >>>> pathways  of individual molecules inside living cells. They can see
> >>>> how molecules  create synapses between nerve cells in the brain; they
> >>>> can track proteins  involved in Parkinson¹s, Alzheimer¹s and
> >>>> Huntington¹s diseases as they  aggregate; they follow individual
> >>>> proteins in fertilized eggs as these  divide into embryos.
> >>>>
> >>>> It was all but obvious that scientists should ever be able to study
> >>>> living  cells in the tiniest molecular detail. In 1873, the
> >>>> microscopist Ernst Abbe  stipulated a physical limit for the maximum
> >>>> resolution of traditional  optical microscopy: it could never become
> >>>> better than 0.2 micrometres.
> >>>> *Eric Betzig*, *Stefan W. Hell* and *William E. Moerner* are awarded
> >>>> the  Nobel Prize in Chemistry 2014 for having bypassed this limit.
> >>>> Due to their  achievements the optical microscope can now peer into
> >>>> the nanoworld.
> >>>>
> >>>> Two separate principles are rewarded. One enables the method
> >>>> /stimulated  emission depletion (STED) microscopy/, developed by
> >>>> Stefan Hell in 2000.
> >>>> Two laser beams are utilized; one stimulates fluorescent molecules
> >>>> to glow,  another cancels out all fluorescence except for that in a
> >>>> nanometre-sized  volume. Scanning over the sample, nanometre for
> >>>> nanometre, yields an image  with a resolution better than Abbe¹s
> >>>> stipulated limit.
> >>>>
> >>>> Eric Betzig and William Moerner, working separately, laid the
> >>>> foundation  for the second method, /single-molecule microscopy/. The
> >>>> method relies upon  the possibility to turn the fluorescence of
> >>>> individual molecules on and  off. Scientists image the same area
> >>>> multiple times, letting just a few  interspersed molecules glow each
> >>>> time. Superimposing these images yields a  dense super-image resolved
> >>>> at the nanolevel. In 2006 Eric Betzig utilized  this method for the
> >>>> first time.
> >>>>
> >>>> Today, nanoscopy is used world-wide and new knowledge of greatest
> >>>> benefit  to mankind is produced on a daily basis.
> >>>>
> >>>>
> >>>> //
> >>>>
> >>>> My thanks to the Nobel committee for revealing what W.E. is.
> >>>>
> >>>> --
> >>>>
> >>>>
> >>>>
> >>>> George McNamara, Ph.D.
> >>>> Single Cells Analyst
> >>>> L.J.N. Cooper Lab
> >>>> University of Texas M.D. Anderson Cancer Center  Houston, TX 77054
> >>>> Tattletales
> >>>> http://scanmail.trustwave.com/?c=129&d=ksa11D595nB9lxX7ILvNZUmUIR93pR
> >>>> N_e
> >>>> ePLeAaSbQ&u=http%3a%2f%2fworks%2ebepress%2ecom%2fgmcnamara%2f42
> >>>>
> >>
> >> --
> >> Martin Wessendorf, Ph.D.                   office: [phone]
> >> Assoc Prof, Dept Neuroscience                 lab: [phone]
> >> University of Minnesota             Preferred FAX: [phone]
> >> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> >> Minneapolis, MN  55455                    e-mail: [hidden email]
>