congratulations to Michael Davidson and his team for getting a large number of FP plasmids into addgene.org

[George McNamara]

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congratulations to Michael Davidson and his team for getting a large
number of FP plasmids into addgene.org

Plasmids:
https://www.addgene.org/Michael_Davidson/

the addgene news story:

Michael Davidson <https://www.addgene.org/Michael_Davidson/> and his lab
from Florida State University have contributed their comprehensive
collection of ORFs tagged with a variety of fluorescent proteins
<https://www.addgene.org/fluorescent_proteins/davidson/>. In addition to
this collection of ORFs, over 100 empty backbones are available from the
Davidson lab for tagging your gene of interest.

The Davidson plasmid collection includes excitation/emission,
localization, sequence, and supplemental information for many of the
plasmids so you can easily find what you need.

We currently have ~ 300 plasmids (empty backbones and mEmerald tagged
ORFs) available for request, with more plasmids becoming available
everyday. Browse the collection here
<https://www.addgene.org/fluorescent_proteins/davidson/>.

FP Guide at addgene
https://www.addgene.org/fluorescent_proteins/

I also want to give a shout out to Loren Looger for posting mEos4
(though both bacterial expression)
pRSETa_ mEos4a <https://www.addgene.org/51072/> (Plasmid #51072)
pRSETa_ mEos4b <https://www.addgene.org/51073/> (Plasmid #51073)
Douglas Kim and others for posting many versions of GCaMP6 (see online
for more)
pGP-CMV-GCaMP6f <https://www.addgene.org/40755/> (Plasmid #40755)
pGP-CMV-GCaMP6m <https://www.addgene.org/40754/> (Plasmid #40754)
pGP-CMV-GCaMP6s <https://www.addgene.org/40753/> (Plasmid #40753)

Wolf Frommer, Robert Campbell, Loren & Douglas, and more, for FP
biosensors at addgene
https://www.addgene.org/fluorescent_proteins/biosensors/

More on FP biosensors at Wolf's site
http://biosensor.dpb.carnegiescience.edu/biosensors
and Newman, fosbrink and Zhang's Excel file supplement (free to download)
http://pubs.acs.org/doi/suppl/10.1021/cr100002u
old data in the FPaa file, and of course older spectra, freely available at
http://works.bepress.com/gmcnamara/9/


On the flip side, the FP field is falling way behind the CRISPR/Cas
(really Cas9/sgRNA) and TALEN field in terms of accessible plasmid and kits.
https://www.addgene.org/Genome_Engineering/


best wishes and may all your photons be bright and electrons be clean,

George


--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/

[phil laissue-2]

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great resource, thanks for pointing that out!
Best wishes,
Philippe

_________________________________________
Philippe Laissue, PhD, Director of Bioimaging Unit
School of Biological Sciences, Room 4.17
University of Essex, Colchester CO4 3SQ, UK
(0044) 01206 872246 / (0044) 07842 676 456
[hidden email]
privatewww.essex.ac.uk/~plaissue


On 20 June 2014 03:13, George McNamara <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> congratulations to Michael Davidson and his team for getting a large
> number of FP plasmids into addgene.org
>
> Plasmids:
> https://www.addgene.org/Michael_Davidson/
>
> the addgene news story:
>
> Michael Davidson <https://www.addgene.org/Michael_Davidson/> and his lab
> from Florida State University have contributed their comprehensive
> collection of ORFs tagged with a variety of fluorescent proteins <
> https://www.addgene.org/fluorescent_proteins/davidson/>. In addition to
> this collection of ORFs, over 100 empty backbones are available from the
> Davidson lab for tagging your gene of interest.
>
> The Davidson plasmid collection includes excitation/emission,
> localization, sequence, and supplemental information for many of the
> plasmids so you can easily find what you need.
>
> We currently have ~ 300 plasmids (empty backbones and mEmerald tagged
> ORFs) available for request, with more plasmids becoming available
> everyday. Browse the collection here <https://www.addgene.org/
> fluorescent_proteins/davidson/>.
>
> FP Guide at addgene
> https://www.addgene.org/fluorescent_proteins/
>
> I also want to give a shout out to Loren Looger for posting mEos4 (though
> both bacterial expression)
> pRSETa_ mEos4a <https://www.addgene.org/51072/> (Plasmid #51072)
> pRSETa_ mEos4b <https://www.addgene.org/51073/> (Plasmid #51073)
> Douglas Kim and others for posting many versions of GCaMP6 (see online for
> more)
> pGP-CMV-GCaMP6f <https://www.addgene.org/40755/> (Plasmid #40755)
> pGP-CMV-GCaMP6m <https://www.addgene.org/40754/> (Plasmid #40754)
> pGP-CMV-GCaMP6s <https://www.addgene.org/40753/> (Plasmid #40753)
>
> Wolf Frommer, Robert Campbell, Loren & Douglas, and more, for FP
> biosensors at addgene
> https://www.addgene.org/fluorescent_proteins/biosensors/
>
> More on FP biosensors at Wolf's site
> http://biosensor.dpb.carnegiescience.edu/biosensors
> and Newman, fosbrink and Zhang's Excel file supplement (free to download)
> http://pubs.acs.org/doi/suppl/10.1021/cr100002u
> old data in the FPaa file, and of course older spectra, freely available at
> http://works.bepress.com/gmcnamara/9/
>
>
> On the flip side, the FP field is falling way behind the CRISPR/Cas
> (really Cas9/sgRNA) and TALEN field in terms of accessible plasmid and kits.
> https://www.addgene.org/Genome_Engineering/
>
>
> best wishes and may all your photons be bright and electrons be clean,
>
> George
>
>
> --
>
>
>
> George McNamara, Ph.D.
> Single Cells Analyst
> L.J.N. Cooper Lab
> University of Texas M.D. Anderson Cancer Center
> Houston, TX 77054
> Tattletales http://works.bepress.com/gmcnamara/26/
>