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I cannot help much with the laser power question but it looks the
objectives on the Vutara system are not Leica but Olympus, judging from
the silicon immersion objective listing and having a 60x, not 63x lens.
If the 500 mW safe power (according to Nikon) is for an expanded beam
that fills the back aperture of the objective and your 400 mW beam is
focused in small spot in or near the back focal plane of the objective to
illuminate the whole field of view, then you definitely have much higher
power density in that spot. The beam may need to be expanded somewhat
(of course then you will illuminate a smaller area in the sample).
The manufacturer should be able to tell you what is the damage threshold
for the objective - watts per square mm or something like that.
Stan Vitha
Microscopy and Imaging Center
Texas A&M University
On Tue, 10 Mar 2015 22:26:43 +0000, Nuno Moreno
<
[hidden email]> wrote:
>
>Dear Mic Folks
>
>Not being a confocal question I would like to getter your opinion
regarding power in an objective. I got “unofficially� from nikon that
the 100x 1.45NA lens should hold 500mW with no problems. Further,
systems like vutara uses 1W lasers (from leica I suppose from the
objective list)! The problem is that I used less than that (<400mW) and
with a few hours of usage I got 2 damage objectives!!! They start to get
like a deposition inside the internal lenses. This is being a nightmare as you
might expect: very expensive objectives and the system out of order.
I’m actually assuming Nikon will repair this for free (or almost) but one
never knows.
>
>The first one I thought it was a manufacturing defect but the second, a
water objetive…let me conclude I was having a "damaging pattern"
>
>Did anyone have a similar problem?
>
>Kind regards and many thanks
>Nuno Moreno
>srii.info
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