digital cameras
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One of our labs is planning to buy a color digital camera for
bright-field and fluorescence pictures. They are considering
the PAXcam2+ and Infinity X32. Does anyone have any experience with
either of these two color cameras or any other camera suggestions?
Cytometry Facility Huck Institute of the Life Sciences 319 Life Sciences Building Penn State University University Park, PA 16802 http://www.huck.psu.edu/facilities/cytometry-up/ 814-863-2762 |
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Elaine - the X32 will certainly give you greater image size flexibility..
Bill Miller
On Fri, May 8, 2009 at 2:51 PM, Elaine Kunze <[hidden email]> wrote:
-- Bill Miller 860-672-0068 [hidden email] |
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In reply to this post by Elaine Kunze
Hello Elaine, I would recommend a SPOT RT Slider, or something similar. It is a Monochrome camera with a bayer filter slider that you can remove for fluorescence. Monochrome cameras are always better for fluorescence (bayer filters always eat a lot of light) with higher Quantum Efficiencies. Pete On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: One of our labs is planning to buy a color digital camera for bright-field and fluorescence pictures. They are considering the PAXcam2+ and Infinity X32. Does anyone have any experience with either of these two color cameras or any other camera suggestions? Elaine Kunze |
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Hi Elaine, I concur with Peter. I have been using SPOT RT (and Pursuit)
Sliders for the last 4 years. They work great, very sensitive for fluorescence
and take good quality colour images. Basically you get two cameras in one. The
software they use is simple and fairly intuitive to use. I don’t think i have
ever had a user say that it was difficult to use. Cheers Cam Cameron J. Nowell Office: +61 3 9341 3155 From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Peter Pitrone Hello Elaine, I would recommend a SPOT RT Slider, or
something similar. It is a Monochrome camera with a bayer filter slider
that you can remove for fluorescence. Monochrome cameras are always better for
fluorescence (bayer filters always eat a lot of light) with higher
Quantum Efficiencies. Pete On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
Elaine Kunze No virus
found in this incoming message. This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waiver any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
 
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allison van-de-meene (RRes-Roth) |
Re: digital cameras
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Hi Elaine,
We have a QImaging Retiga Exi monochrome camera with an RGB filter wheel that can slide in and out. It works really well for fluorescence and the colours for brightfield were the best we saw for our applications after testing a range of cameras. Best wishes, Allison ________________________________ From: Confocal Microscopy List on behalf of Cameron Nowell Sent: Mon 5/11/2009 7:42 AM To: [hidden email] Subject: Re: digital cameras Hi Elaine, I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders for the last 4 years. They work great, very sensitive for fluorescence and take good quality colour images. Basically you get two cameras in one. The software they use is simple and fairly intuitive to use. I don't think i have ever had a user say that it was difficult to use. Cheers Cam Cameron J. Nowell Microscopy Manager Centre for Advanced Microscopy Ludwig Institute for Cancer Research PO Box 2008 Royal Melbourne Hospital Victoria, 3050 AUSTRALIA Office: +61 3 9341 3155 Mobile: +61422882700 Fax: +61 3 9341 3104 Facility Website <http://www.ludwig.edu.au/branch/research/platform/microscopy.htm> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter Pitrone Sent: Monday, 11 May 2009 4:34 PM To: [hidden email] Subject: Re: digital cameras Hello Elaine, I would recommend a SPOT RT Slider, or something similar. It is a Monochrome camera with a bayer filter slider that you can remove for fluorescence. Monochrome cameras are always better for fluorescence (bayer filters always eat a lot of light) with higher Quantum Efficiencies. Pete On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: One of our labs is planning to buy a color digital camera for bright-field and fluorescence pictures. They are considering the PAXcam2+ and Infinity X32. Does anyone have any experience with either of these two color cameras or any other camera suggestions? Elaine Kunze Cytometry Facility Huck Institute of the Life Sciences 319 Life Sciences Building Penn State University University Park, PA 16802 http://www.huck.psu.edu/facilities/cytometry-up/ 814-863-2762 No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: 05/10/09 07:02:00 ________________________________ This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waiver any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
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In reply to this post by Cameron Nowell
Dear Elaine,
Along the same approach, CRI makes tunable filters that can be fitted to most microscopes to allow RGB brightfield images with any monochrome camera. then you still have the ability for fluorescence, provided your camera has a QE curve and sufficient cooling for long exposures (or you have bright fluorescent labels). Such filters can be readily controlled by most acquisition software and provide automatic white balance to set exposure times. With most acquisition software, you can also set the fluorescent filters for sequential acquisition of red, green and blue channels of a brightfield image. Although, white balance may not be automated. Regards, Glen On May 10, 2009, at 11:42 PM, Cameron Nowell wrote: > Hi Elaine, > > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders > for the last 4 years. They work great, very sensitive for > fluorescence and take good quality colour images. Basically you get > two cameras in one. The software they use is simple and fairly > intuitive to use. I don’t think i have ever had a user say that it > was difficult to use. > > > Cheers > > Cam > > > > Cameron J. Nowell > Microscopy Manager > Centre for Advanced Microscopy > Ludwig Institute for Cancer Research > PO Box 2008 > Royal Melbourne Hospital > Victoria, 3050 > AUSTRALIA > > Office: +61 3 9341 3155 > Mobile: +61422882700 > Fax: +61 3 9341 3104 > > Facility Website > > > From: Confocal Microscopy List [mailto:[hidden email] > ] On Behalf Of Peter Pitrone > Sent: Monday, 11 May 2009 4:34 PM > To: [hidden email] > Subject: Re: digital cameras > > Hello Elaine, > > I would recommend a SPOT RT Slider, or something similar. It is a > Monochrome camera with a bayer filter slider that you can remove for > fluorescence. Monochrome cameras are always better for fluorescence > (bayer filters always eat a lot of light) with higher Quantum > Efficiencies. > > Pete > > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: > > One of our labs is planning to buy a color digital camera for bright- > field and fluorescence pictures. They are considering the PAXcam2+ > and Infinity X32. Does anyone have any experience with either of > these two color cameras or any other camera suggestions? > > Elaine Kunze > Cytometry Facility > Huck Institute of the Life Sciences > 319 Life Sciences Building > Penn State University > University Park, PA 16802 > http://www.huck.psu.edu/facilities/cytometry-up/ > 814-863-2762 > > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: > 05/10/09 07:02:00 > > > This communication is intended only for the named recipient and may > contain information that is confidential, legally privileged or > subject to copyright; the Ludwig Institute for Cancer Research Ltd > does not waiver any rights if you have received this communication > in error. > The views expressed in this communication are those of the sender > and do not necessarily reflect the views of the Ludwig Institute for > Cancer Research Ltd. > Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] ****************************************************************************** The box said "Requires WindowsXP or better", so I bought a Macintosh. ****************************************************************************** |
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Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi
with a CRI Liquid Crystal Tunable Filter. I think both are viable options. The SPOT system will most likely cost less and I like the SPOT software which is easy to use and free. The Retiga/CRI system is a better system in my humble opinion, and we operate this with Image Pro Plus software. Cheers, Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag ing/Pages/default.aspx -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Glen MacDonald Sent: Monday, May 11, 2009 8:59 AM To: [hidden email] Subject: Re: digital cameras Dear Elaine, Along the same approach, CRI makes tunable filters that can be fitted to most microscopes to allow RGB brightfield images with any monochrome camera. then you still have the ability for fluorescence, provided your camera has a QE curve and sufficient cooling for long exposures (or you have bright fluorescent labels). Such filters can be readily controlled by most acquisition software and provide automatic white balance to set exposure times. With most acquisition software, you can also set the fluorescent filters for sequential acquisition of red, green and blue channels of a brightfield image. Although, white balance may not be automated. Regards, Glen On May 10, 2009, at 11:42 PM, Cameron Nowell wrote: > Hi Elaine, > > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders > for the last 4 years. They work great, very sensitive for > fluorescence and take good quality colour images. Basically you get > two cameras in one. The software they use is simple and fairly > intuitive to use. I don't think i have ever had a user say that it > was difficult to use. > > > Cheers > > Cam > > > > Cameron J. Nowell > Microscopy Manager > Centre for Advanced Microscopy > Ludwig Institute for Cancer Research > PO Box 2008 > Royal Melbourne Hospital > Victoria, 3050 > AUSTRALIA > > Office: +61 3 9341 3155 > Mobile: +61422882700 > Fax: +61 3 9341 3104 > > Facility Website > > > From: Confocal Microscopy List > ] On Behalf Of Peter Pitrone > Sent: Monday, 11 May 2009 4:34 PM > To: [hidden email] > Subject: Re: digital cameras > > Hello Elaine, > > I would recommend a SPOT RT Slider, or something similar. It is a > Monochrome camera with a bayer filter slider that you can remove for > fluorescence. Monochrome cameras are always better for fluorescence > (bayer filters always eat a lot of light) with higher Quantum > Efficiencies. > > Pete > > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: > > One of our labs is planning to buy a color digital camera for bright- > field and fluorescence pictures. They are considering the PAXcam2+ > and Infinity X32. Does anyone have any experience with either of > these two color cameras or any other camera suggestions? > > Elaine Kunze > Cytometry Facility > Huck Institute of the Life Sciences > 319 Life Sciences Building > Penn State University > University Park, PA 16802 > http://www.huck.psu.edu/facilities/cytometry-up/ > 814-863-2762 > > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: > 05/10/09 07:02:00 > > > This communication is intended only for the named recipient and may > contain information that is confidential, legally privileged or > subject to copyright; the Ludwig Institute for Cancer Research Ltd > does not waiver any rights if you have received this communication > in error. > The views expressed in this communication are those of the sender > and do not necessarily reflect the views of the Ludwig Institute for > Cancer Research Ltd. > Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] ************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ****** --------------------------------------------------------------------- SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. --------------------------------------------------------------------- |
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Thanks Brian, and on that note, the newer RGB Sliders from QImaging have three ports, a clear port for most fluro, a clear port w/ IR blocking, and the LCF port. In addition, the Q filter is easily rotatable with respect to the camera body, to eliminate polarization interference. The filter is controlled thru the Q camera, or can be used with other cameras via a CRI control box.
dave
On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian <[hidden email]> wrote: Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi |
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Just a minor correction for the archives more than anything else. If I'm not mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an LCD RGB color changing filter, so that three shot colour images may be taken at full chip resolution.
Cheers
Steve Cody
2009/5/12 David Barnes <[hidden email]>
-- Stephen H. Cody |
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Steve, you are right, the SPOT RT uses a LCD.
If one already has to capture 3 images, I am wondering if it would be a good idea to use a monochrome camera plus three R/G/B filters sitting in the fluorescence filter turret. The acquisition software can be set up in a way that it takes three images and changes the filters in between. Not the fastest solution, but offers high resolution and sensitivity w/o the need for an additional b/w camera. One just needs a filter turret and three filters... Michael > Just a minor correction for the archives more than anything else. If I'm > not > mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an > LCD RGB color changing filter, so that three shot colour images may be > taken > at full chip resolution. > > Cheers > Steve Cody > 2009/5/12 David Barnes <[hidden email]> > >> Thanks Brian, and on that note, the newer RGB Sliders from QImaging have >> three ports, a clear port for most fluro, a clear port w/ IR blocking, >> and >> the LCF port. In addition, the Q filter is easily rotatable with respect >> to >> the camera body, to eliminate polarization interference. The filter is >> controlled thru the Q camera, or can be used with other cameras via a >> CRI >> control box. >> >> dave >> >> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian >> <[hidden email]>wrote: >> >>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi >>> with a CRI Liquid Crystal Tunable Filter. I think both are viable >>> options. The SPOT system will most likely cost less and I like the SPOT >>> software which is easy to use and free. The Retiga/CRI system is a >>> better system in my humble opinion, and we operate this with Image Pro >>> Plus software. >>> Cheers, >>> >>> >>> Brian D Armstrong PhD >>> Light Microscopy Core Manager >>> Beckman Research Institute >>> City of Hope >>> Dept of Neuroscience >>> 1450 E Duarte Rd >>> Duarte, CA 91010 >>> 626-256-4673 x62872 >>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag >>> ing/Pages/default.aspx >>> -----Original Message----- >>> From: Confocal Microscopy List >>> [mailto:[hidden email]] >>> On Behalf Of Glen MacDonald >>> Sent: Monday, May 11, 2009 8:59 AM >>> To: [hidden email] >>> Subject: Re: digital cameras >>> >>> Dear Elaine, >>> Along the same approach, CRI makes tunable filters that can be fitted >>> to most microscopes to allow RGB brightfield images with any >>> monochrome camera. then you still have the ability for fluorescence, >>> provided your camera has a QE curve and sufficient cooling for long >>> exposures (or you have bright fluorescent labels). Such filters can >>> be readily controlled by most acquisition software and provide >>> automatic white balance to set exposure times. With most acquisition >>> software, you can also set the fluorescent filters for sequential >>> acquisition of red, green and blue channels of a brightfield image. >>> Although, white balance may not be automated. >>> >>> Regards, >>> Glen >>> >>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote: >>> >>> > Hi Elaine, >>> > >>> > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders >>> > for the last 4 years. They work great, very sensitive for >>> > fluorescence and take good quality colour images. Basically you get >>> > two cameras in one. The software they use is simple and fairly >>> > intuitive to use. I don't think i have ever had a user say that it >>> > was difficult to use. >>> > >>> > >>> > Cheers >>> > >>> > Cam >>> > >>> > >>> > >>> > Cameron J. Nowell >>> > Microscopy Manager >>> > Centre for Advanced Microscopy >>> > Ludwig Institute for Cancer Research >>> > PO Box 2008 >>> > Royal Melbourne Hospital >>> > Victoria, 3050 >>> > AUSTRALIA >>> > >>> > Office: +61 3 9341 3155 >>> > Mobile: +61422882700 >>> > Fax: +61 3 9341 3104 >>> > >>> > Facility Website >>> > >>> > >>> > From: Confocal Microscopy List >>> [mailto:[hidden email] >>> > ] On Behalf Of Peter Pitrone >>> > Sent: Monday, 11 May 2009 4:34 PM >>> > To: [hidden email] >>> > Subject: Re: digital cameras >>> > >>> > Hello Elaine, >>> > >>> > I would recommend a SPOT RT Slider, or something similar. It is a >>> > Monochrome camera with a bayer filter slider that you can remove for >>> > fluorescence. Monochrome cameras are always better for fluorescence >>> > (bayer filters always eat a lot of light) with higher Quantum >>> > Efficiencies. >>> > >>> > Pete >>> > >>> > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: >>> > >>> > One of our labs is planning to buy a color digital camera for bright- >>> > field and fluorescence pictures. They are considering the PAXcam2+ >>> > and Infinity X32. Does anyone have any experience with either of >>> > these two color cameras or any other camera suggestions? >>> > >>> > Elaine Kunze >>> > Cytometry Facility >>> > Huck Institute of the Life Sciences >>> > 319 Life Sciences Building >>> > Penn State University >>> > University Park, PA 16802 >>> > http://www.huck.psu.edu/facilities/cytometry-up/ >>> > 814-863-2762 >>> > >>> > >>> > No virus found in this incoming message. >>> > Checked by AVG - www.avg.com >>> > Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: >>> > 05/10/09 07:02:00 >>> > >>> > >>> > This communication is intended only for the named recipient and may >>> > contain information that is confidential, legally privileged or >>> > subject to copyright; the Ludwig Institute for Cancer Research Ltd >>> > does not waiver any rights if you have received this communication >>> > in error. >>> > The views expressed in this communication are those of the sender >>> > and do not necessarily reflect the views of the Ludwig Institute for >>> > Cancer Research Ltd. >>> > >>> >>> >>> >>> Glen MacDonald >>> Core for Communication Research >>> Virginia Merrill Bloedel Hearing Research Center >>> Box 357923 >>> University of Washington >>> Seattle, WA 98195-7923 USA >>> (206) 616-4156 >>> [hidden email] >>> >>> ************************************************************************ >>> ****** >>> The box said "Requires WindowsXP or better", so I bought a Macintosh. >>> ************************************************************************ >>> ****** >>> >>> >>> --------------------------------------------------------------------- >>> >>> SECURITY/CONFIDENTIALITY WARNING: >>> This message and any attachments are intended solely for the individual >>> or >>> entity to which they are addressed. This communication may contain >>> information that is privileged, confidential, or exempt from disclosure >>> under applicable law (e.g., personal health information, research data, >>> financial information). Because this e-mail has been sent without >>> encryption, individuals other than the intended recipient may be able >>> to >>> view the information, forward it to others or tamper with the >>> information >>> without the knowledge or consent of the sender. If you are not the >>> intended >>> recipient, or the employee or person responsible for delivering the >>> message >>> to the intended recipient, any dissemination, distribution or copying >>> of the >>> communication is strictly prohibited. If you received the communication >>> in >>> error, please notify the sender immediately by replying to this message >>> and >>> deleting the message and any accompanying files from your system. If, >>> due to >>> the security risks, you do not wish to receive further communications >>> via >>> e-mail, please reply to this message and inform the sender that you do >>> not >>> wish to receive further e-mail from the sender. >>> --------------------------------------------------------------------- >>> >> >> > > > -- > Stephen H. Cody |
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Hi Michael,
We have a Spot-RT. Typically the LCD-RGB filters are not used for fluorescence capture, only for brightfield-color (natural/stained), using the normal fluorescence "cubes" to produce fluorescence and filter it, and the camera is operated in monochrome mode for that. The Spot software makes it very easy to merge multiple separately acquired images - as in R,G, and B emission + DIC. However, the movement of the sliding fluorescence cubes produces some errors in registration for the most critical work, so we also use a system including a polychroic in the "epi-cube" position and automated excitation and emission filter wheels (Ludl)that can be changed without image registration issues. The system also has a motorized focus (Prior) for multicolor fluorescence stack collection. All of this is currently run by Compix SimplePCI although I hope to switch to MicroManager as soon as a Spot-RT driver is available :-( Dale Umass@Amherst Michael Weber wrote: > Steve, you are right, the SPOT RT uses a LCD. > > If one already has to capture 3 images, I am wondering if it would be a > good idea to use a monochrome camera plus three R/G/B filters sitting in > the fluorescence filter turret. The acquisition software can be set up in > a way that it takes three images and changes the filters in between. Not > the fastest solution, but offers high resolution and sensitivity w/o the > need for an additional b/w camera. One just needs a filter turret and > three filters... > > Michael > > >> Just a minor correction for the archives more than anything else. If I'm >> not >> mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an >> LCD RGB color changing filter, so that three shot colour images may be >> taken >> at full chip resolution. >> >> Cheers >> Steve Cody >> 2009/5/12 David Barnes <[hidden email]> >> >>> Thanks Brian, and on that note, the newer RGB Sliders from QImaging have >>> three ports, a clear port for most fluro, a clear port w/ IR blocking, >>> and >>> the LCF port. In addition, the Q filter is easily rotatable with respect >>> to >>> the camera body, to eliminate polarization interference. The filter is >>> controlled thru the Q camera, or can be used with other cameras via a >>> CRI >>> control box. >>> >>> dave >>> >>> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian >>> <[hidden email]>wrote: >>> >>>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi >>>> with a CRI Liquid Crystal Tunable Filter. I think both are viable >>>> options. The SPOT system will most likely cost less and I like the SPOT >>>> software which is easy to use and free. The Retiga/CRI system is a >>>> better system in my humble opinion, and we operate this with Image Pro >>>> Plus software. >>>> Cheers, >>>> >>>> >>>> Brian D Armstrong PhD >>>> Light Microscopy Core Manager >>>> Beckman Research Institute >>>> City of Hope >>>> Dept of Neuroscience >>>> 1450 E Duarte Rd >>>> Duarte, CA 91010 >>>> 626-256-4673 x62872 >>>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag >>>> ing/Pages/default.aspx >>>> -----Original Message----- >>>> From: Confocal Microscopy List >>>> [mailto:[hidden email]] >>>> On Behalf Of Glen MacDonald >>>> Sent: Monday, May 11, 2009 8:59 AM >>>> To: [hidden email] >>>> Subject: Re: digital cameras >>>> >>>> Dear Elaine, >>>> Along the same approach, CRI makes tunable filters that can be fitted >>>> to most microscopes to allow RGB brightfield images with any >>>> monochrome camera. then you still have the ability for fluorescence, >>>> provided your camera has a QE curve and sufficient cooling for long >>>> exposures (or you have bright fluorescent labels). Such filters can >>>> be readily controlled by most acquisition software and provide >>>> automatic white balance to set exposure times. With most acquisition >>>> software, you can also set the fluorescent filters for sequential >>>> acquisition of red, green and blue channels of a brightfield image. >>>> Although, white balance may not be automated. >>>> >>>> Regards, >>>> Glen >>>> >>>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote: >>>> >>>>> Hi Elaine, >>>>> >>>>> I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders >>>>> for the last 4 years. They work great, very sensitive for >>>>> fluorescence and take good quality colour images. Basically you get >>>>> two cameras in one. The software they use is simple and fairly >>>>> intuitive to use. I don't think i have ever had a user say that it >>>>> was difficult to use. >>>>> >>>>> >>>>> Cheers >>>>> >>>>> Cam >>>>> >>>>> >>>>> >>>>> Cameron J. Nowell >>>>> Microscopy Manager >>>>> Centre for Advanced Microscopy >>>>> Ludwig Institute for Cancer Research >>>>> PO Box 2008 >>>>> Royal Melbourne Hospital >>>>> Victoria, 3050 >>>>> AUSTRALIA >>>>> >>>>> Office: +61 3 9341 3155 >>>>> Mobile: +61422882700 >>>>> Fax: +61 3 9341 3104 >>>>> >>>>> Facility Website >>>>> >>>>> >>>>> From: Confocal Microscopy List >>>> [mailto:[hidden email] >>>>> ] On Behalf Of Peter Pitrone >>>>> Sent: Monday, 11 May 2009 4:34 PM >>>>> To: [hidden email] >>>>> Subject: Re: digital cameras >>>>> >>>>> Hello Elaine, >>>>> >>>>> I would recommend a SPOT RT Slider, or something similar. It is a >>>>> Monochrome camera with a bayer filter slider that you can remove for >>>>> fluorescence. Monochrome cameras are always better for fluorescence >>>>> (bayer filters always eat a lot of light) with higher Quantum >>>>> Efficiencies. >>>>> >>>>> Pete >>>>> >>>>> On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: >>>>> >>>>> One of our labs is planning to buy a color digital camera for bright- >>>>> field and fluorescence pictures. They are considering the PAXcam2+ >>>>> and Infinity X32. Does anyone have any experience with either of >>>>> these two color cameras or any other camera suggestions? >>>>> >>>>> Elaine Kunze >>>>> Cytometry Facility >>>>> Huck Institute of the Life Sciences >>>>> 319 Life Sciences Building >>>>> Penn State University >>>>> University Park, PA 16802 >>>>> http://www.huck.psu.edu/facilities/cytometry-up/ >>>>> 814-863-2762 >>>>> >>>>> >>>>> No virus found in this incoming message. >>>>> Checked by AVG - www.avg.com >>>>> Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: >>>>> 05/10/09 07:02:00 >>>>> >>>>> >>>>> This communication is intended only for the named recipient and may >>>>> contain information that is confidential, legally privileged or >>>>> subject to copyright; the Ludwig Institute for Cancer Research Ltd >>>>> does not waiver any rights if you have received this communication >>>>> in error. >>>>> The views expressed in this communication are those of the sender >>>>> and do not necessarily reflect the views of the Ludwig Institute for >>>>> Cancer Research Ltd. >>>>> >>>> >>>> >>>> Glen MacDonald >>>> Core for Communication Research >>>> Virginia Merrill Bloedel Hearing Research Center >>>> Box 357923 >>>> University of Washington >>>> Seattle, WA 98195-7923 USA >>>> (206) 616-4156 >>>> [hidden email] >>>> >>>> ************************************************************************ >>>> ****** >>>> The box said "Requires WindowsXP or better", so I bought a Macintosh. >>>> ************************************************************************ >>>> ****** >>>> >>>> >>>> --------------------------------------------------------------------- >>>> >>>> SECURITY/CONFIDENTIALITY WARNING: >>>> This message and any attachments are intended solely for the individual >>>> or >>>> entity to which they are addressed. This communication may contain >>>> information that is privileged, confidential, or exempt from disclosure >>>> under applicable law (e.g., personal health information, research data, >>>> financial information). Because this e-mail has been sent without >>>> encryption, individuals other than the intended recipient may be able >>>> to >>>> view the information, forward it to others or tamper with the >>>> information >>>> without the knowledge or consent of the sender. If you are not the >>>> intended >>>> recipient, or the employee or person responsible for delivering the >>>> message >>>> to the intended recipient, any dissemination, distribution or copying >>>> of the >>>> communication is strictly prohibited. If you received the communication >>>> in >>>> error, please notify the sender immediately by replying to this message >>>> and >>>> deleting the message and any accompanying files from your system. If, >>>> due to >>>> the security risks, you do not wish to receive further communications >>>> via >>>> e-mail, please reply to this message and inform the sender that you do >>>> not >>>> wish to receive further e-mail from the sender. >>>> --------------------------------------------------------------------- >>>> >>> >> >> -- >> Stephen H. Cody |
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In reply to this post by Michael Weber-4
Ok, Glen mentioned that already. Forget about it then.
> If one already has to capture 3 images, I am wondering if it would be a > good idea to use a monochrome camera plus three R/G/B filters sitting in > the fluorescence filter turret. The acquisition software can be set up in > a way that it takes three images and changes the filters in between. >> Just a minor correction for the archives more than anything else. If I'm >> not >> mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an >> LCD RGB color changing filter, so that three shot colour images may be >> taken >> at full chip resolution. >> >> Cheers >> Steve Cody >> 2009/5/12 David Barnes <[hidden email]> >> >>> Thanks Brian, and on that note, the newer RGB Sliders from QImaging >>> have >>> three ports, a clear port for most fluro, a clear port w/ IR blocking, >>> and >>> the LCF port. In addition, the Q filter is easily rotatable with >>> respect >>> to >>> the camera body, to eliminate polarization interference. The filter is >>> controlled thru the Q camera, or can be used with other cameras via a >>> CRI >>> control box. >>> >>> dave >>> >>> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian >>> <[hidden email]>wrote: >>> >>>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga >>>> EXi >>>> with a CRI Liquid Crystal Tunable Filter. I think both are viable >>>> options. The SPOT system will most likely cost less and I like the >>>> SPOT >>>> software which is easy to use and free. The Retiga/CRI system is a >>>> better system in my humble opinion, and we operate this with Image Pro >>>> Plus software. >>>> Cheers, >>>> >>>> >>>> Brian D Armstrong PhD >>>> Light Microscopy Core Manager >>>> Beckman Research Institute >>>> City of Hope >>>> Dept of Neuroscience >>>> 1450 E Duarte Rd >>>> Duarte, CA 91010 >>>> 626-256-4673 x62872 >>>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag >>>> ing/Pages/default.aspx >>>> -----Original Message----- >>>> From: Confocal Microscopy List >>>> [mailto:[hidden email]] >>>> On Behalf Of Glen MacDonald >>>> Sent: Monday, May 11, 2009 8:59 AM >>>> To: [hidden email] >>>> Subject: Re: digital cameras >>>> >>>> Dear Elaine, >>>> Along the same approach, CRI makes tunable filters that can be fitted >>>> to most microscopes to allow RGB brightfield images with any >>>> monochrome camera. then you still have the ability for fluorescence, >>>> provided your camera has a QE curve and sufficient cooling for long >>>> exposures (or you have bright fluorescent labels). Such filters can >>>> be readily controlled by most acquisition software and provide >>>> automatic white balance to set exposure times. With most acquisition >>>> software, you can also set the fluorescent filters for sequential >>>> acquisition of red, green and blue channels of a brightfield image. >>>> Although, white balance may not be automated. >>>> >>>> Regards, >>>> Glen >>>> >>>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote: >>>> >>>> > Hi Elaine, >>>> > >>>> > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders >>>> > for the last 4 years. They work great, very sensitive for >>>> > fluorescence and take good quality colour images. Basically you get >>>> > two cameras in one. The software they use is simple and fairly >>>> > intuitive to use. I don't think i have ever had a user say that it >>>> > was difficult to use. >>>> > >>>> > >>>> > Cheers >>>> > >>>> > Cam >>>> > >>>> > >>>> > >>>> > Cameron J. Nowell >>>> > Microscopy Manager >>>> > Centre for Advanced Microscopy >>>> > Ludwig Institute for Cancer Research >>>> > PO Box 2008 >>>> > Royal Melbourne Hospital >>>> > Victoria, 3050 >>>> > AUSTRALIA >>>> > >>>> > Office: +61 3 9341 3155 >>>> > Mobile: +61422882700 >>>> > Fax: +61 3 9341 3104 >>>> > >>>> > Facility Website >>>> > >>>> > >>>> > From: Confocal Microscopy List >>>> [mailto:[hidden email] >>>> > ] On Behalf Of Peter Pitrone >>>> > Sent: Monday, 11 May 2009 4:34 PM >>>> > To: [hidden email] >>>> > Subject: Re: digital cameras >>>> > >>>> > Hello Elaine, >>>> > >>>> > I would recommend a SPOT RT Slider, or something similar. It is a >>>> > Monochrome camera with a bayer filter slider that you can remove for >>>> > fluorescence. Monochrome cameras are always better for fluorescence >>>> > (bayer filters always eat a lot of light) with higher Quantum >>>> > Efficiencies. >>>> > >>>> > Pete >>>> > >>>> > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote: >>>> > >>>> > One of our labs is planning to buy a color digital camera for >>>> bright- >>>> > field and fluorescence pictures. They are considering the PAXcam2+ >>>> > and Infinity X32. Does anyone have any experience with either of >>>> > these two color cameras or any other camera suggestions? >>>> > >>>> > Elaine Kunze >>>> > Cytometry Facility >>>> > Huck Institute of the Life Sciences >>>> > 319 Life Sciences Building >>>> > Penn State University >>>> > University Park, PA 16802 >>>> > http://www.huck.psu.edu/facilities/cytometry-up/ >>>> > 814-863-2762 >>>> > >>>> > >>>> > No virus found in this incoming message. >>>> > Checked by AVG - www.avg.com >>>> > Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date: >>>> > 05/10/09 07:02:00 >>>> > >>>> > >>>> > This communication is intended only for the named recipient and may >>>> > contain information that is confidential, legally privileged or >>>> > subject to copyright; the Ludwig Institute for Cancer Research Ltd >>>> > does not waiver any rights if you have received this communication >>>> > in error. >>>> > The views expressed in this communication are those of the sender >>>> > and do not necessarily reflect the views of the Ludwig Institute for >>>> > Cancer Research Ltd. >>>> > >>>> >>>> >>>> >>>> Glen MacDonald >>>> Core for Communication Research >>>> Virginia Merrill Bloedel Hearing Research Center >>>> Box 357923 >>>> University of Washington >>>> Seattle, WA 98195-7923 USA >>>> (206) 616-4156 >>>> [hidden email] >>>> >>>> ************************************************************************ >>>> ****** >>>> The box said "Requires WindowsXP or better", so I bought a Macintosh. >>>> ************************************************************************ >>>> ****** >>>> >>>> >>>> --------------------------------------------------------------------- >>>> >>>> SECURITY/CONFIDENTIALITY WARNING: >>>> This message and any attachments are intended solely for the >>>> individual >>>> or >>>> entity to which they are addressed. This communication may contain >>>> information that is privileged, confidential, or exempt from >>>> disclosure >>>> under applicable law (e.g., personal health information, research >>>> data, >>>> financial information). Because this e-mail has been sent without >>>> encryption, individuals other than the intended recipient may be able >>>> to >>>> view the information, forward it to others or tamper with the >>>> information >>>> without the knowledge or consent of the sender. If you are not the >>>> intended >>>> recipient, or the employee or person responsible for delivering the >>>> message >>>> to the intended recipient, any dissemination, distribution or copying >>>> of the >>>> communication is strictly prohibited. If you received the >>>> communication >>>> in >>>> error, please notify the sender immediately by replying to this >>>> message >>>> and >>>> deleting the message and any accompanying files from your system. If, >>>> due to >>>> the security risks, you do not wish to receive further communications >>>> via >>>> e-mail, please reply to this message and inform the sender that you do >>>> not >>>> wish to receive further e-mail from the sender. >>>> --------------------------------------------------------------------- >>>> >>> >>> >> >> >> -- >> Stephen H. Cody |
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Just a couple of thoughts on cameras in general. I have used, sold and supported most of the cameras on the market today at one time or another. The tunable RGB LCD filters are a great addition to monochrome cameras, but they are not the be all and end all of color imaging. They do allow you to pick a mono camera that is well suited for your fluorescence work and still get color images. However, the color images take three times as long to capture. These color images are "better" than Bayer filtered images because they have 100% coverage in all three channels, but I have honestly run across very few situations where a Bayer filtered color camera was not truely sufficient for the color work being considered. With that in mind consider that you can buy a low end, high pixel count color camera for not much more than the RGB LCD sliders to go with mono cameras.
It is well worth considering whether a two camera solution might not be a better or more convenient choice. Now that I've commented on cameras let me put the horse back in front of the cart. One COMMON mistake that I have seen too many times to count is buying a fancy camera, only to find that it's software does not do EVERYthing that you need and that the camera is not supported by the third party imaging software you want to use. Although it is very tempting to look at the cameras in a vacuum, I would urge every one to look at the camera and software as a package. It is not fun (and rather inefficient) to use one program for live preview and acuaisition and another for analysis. Evaluate teh software both in terms of your immediate needs and in terms of it's growth potential. Even if all you need at the outset is documentation, consider whether you will ever need measurements or other types of analysis on your images. Then look at the software options again. Also, consider how the camera is supported in any third party software - for example, although a TWAIN driver will give live preview and acquisition capabilities, it will usually be excluded from any automation features of the program. Chris Tully Microscopy and Image Analysis Expert [hidden email] 240-888-1021 http://www.linkedin.com/in/christully On Wed, May 13, 2009 at 9:19 AM, Michael Weber <[hidden email]> wrote: Ok, Glen mentioned that already. Forget about it then. |
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