dihydroethidium and Multiphoton

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Watkins, Simon C Watkins, Simon C
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dihydroethidium and Multiphoton

folks,  has anyone used dihydroethidium (DHE) with mpe excitation? if so what were the ideal conditions? was there much auto-oxidation under MPE exctiation???  I am thinking of using this in some zebrafish expts, so any tips specific to fish would be welcome.

Thanks

S

 

Simon C. Watkins Ph.D, FRC Path

Professor and Vice Chair Cell Biology and Physiology

Professor Immunology Director Center for Biologic Imaging

BSTS 225

University of Pittsburgh

3500 Terrace St

Pittsburgh PA 15261

412-352-2277

www.cbi.pitt.edu