dishes or plates anticorrosive

[Konstantín Levitskiy]

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Dear microscopists.



We have an user who uses iDISCO clarification method. It uses a highly
corrosive media for our bottom glass plastic dishes, and so the plastic has
partially dissolved.

To protect the inverted microscope: what type of material and supplier would
you recommended for the dishes? Thanks in advance.



And just for a bit of fun:

In the middle of an exam, a student asks his teacher: Teacher, if you give
me a passing grade I'll give you a substance that can dissolve anything.

The teacher responds: No, you can keep it

The student baffled retorts: Why not?

And the teacher says: Where would I keep it?

Best regards,

Dr. Konstantín Levitskiy

Servicio de Microscopía

InstitutodeBiomedicinadeSevilla - IBiS

Campus del Hospital Universitario Virgen del Rocío

Avda. Manuel Siurot s/nº

41013 Sevilla

Tlfno: 955 92 3030

Email:  <mailto:[hidden email]> [hidden email]

Web:  <http://www.ibis-sevilla.es/> www.ibis-sevilla.es





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[Moulding, Dale]

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Hi Konstantin,


for BABB we make a well in the dish with sylgard, to keep the babb away from the plastic.

Described here:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226364/

Not perfect and the BABB eventually eats through but good for a couple of imaging sessions. Perhaps it will work for DBE too?


Alternatively we use o-rings superglued in place. These last a long time, the superglue seems to resist BABB. The rings are made from FKM (viton), which is BABB resistant. We get them from polymax.

Looks like EPDM rings resists DBE (according to polymax's chemical compatibility check). So if super glue (or epoxy) holds that may do the trick?


good luck


Dale


http://www.ucl.ac.uk/ich/core-scientific-facilities-centres/confocal-microscopy


________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Konstantín Levitskiy <[hidden email]>
Sent: 22 February 2018 12:04:08
To: [hidden email]
Subject: dishes or plates anticorrosive

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Dear microscopists.



We have an user who uses iDISCO clarification method. It uses a highly
corrosive media for our bottom glass plastic dishes, and so the plastic has
partially dissolved.

To protect the inverted microscope: what type of material and supplier would
you recommended for the dishes? Thanks in advance.



And just for a bit of fun:

In the middle of an exam, a student asks his teacher: Teacher, if you give
me a passing grade I'll give you a substance that can dissolve anything.

The teacher responds: No, you can keep it

The student baffled retorts: Why not?

And the teacher says: Where would I keep it?

Best regards,

Dr. Konstantín Levitskiy

Servicio de Microscopía

InstitutodeBiomedicinadeSevilla - IBiS

Campus del Hospital Universitario Virgen del Rocío

Avda. Manuel Siurot s/nº

41013 Sevilla

Tlfno: 955 92 3030

Email:  <mailto:[hidden email]> [hidden email]

Web:  <http://www.ibis-sevilla.es/> www.ibis-sevilla.es<http://www.ibis-sevilla.es>





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[Dan Focht]

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Dr. Konstantín Levitskiy

Bioptechs makes an interchangeable coverslip dish that very well could solve your problem.
It uses a stainless steel base ring that accepts a 30mm coverslip and provides a 25mm field of view.
An o-ring sealed polycarbonate threaded sleeve screws into the ring sealing the coverslip.
If your solvent would deteriorate the polycarb we can make the sleeve out of PEEK instead.
Your chemicals only make contact with the #1.5 glass coverslip and PEEK.
Replacement coverslips are about $0.33 each.  

Details about it are in this link:
http://www.bioptechs.com/product/interchangeable-coverslip-dish/


Dan



On Feb 22, 2018, at 7:04 AM, Konstantín Levitskiy wrote:

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We have an user who uses iDISCO clarification method. It uses a highly
corrosive media for our bottom glass plastic dishes, and so the plastic has
partially dissolved.

To protect the inverted microscope: what type of material and supplier would
you recommended for the dishes? Thanks in advance.



Best regards,

Dr. Konstantín Levitskiy
Servicio de Microscopía
InstitutodeBiomedicinadeSevilla - IBiS
Campus del Hospital Universitario Virgen del Rocío
Avda. Manuel Siurot s/nº
41013 Sevilla
Tlfno: 955 92 3030
Email:  <mailto:[hidden email]> [hidden email]
Web:  <http://www.ibis-sevilla.es/> www.ibis-sevilla.es
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Dan Focht
Bioptechs Inc.
3560 Beck Road
Butler, PA 16002-9259
 
Office: 724-282-7145
Toll Free: 877-LIVE-CELL (548-3235)
 
[hidden email]
www.bioptechs.com

[Bertrand Vernay-2]

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Dear Konstantin

We happily use the Attofluor dish for cleared sample in BABB/iDISCO (https://www.thermofisher.com/order/catalog/product/A7816)
To keep the sample immobile I place few drops of vacuum grease around the sample and then lower a small piece of ACLAR® cut to size .So far Aclar has been BABB resistant for the duration of the imaging session and it does make the recovery of the sample from the Atto dish easy.
I also add another coverglass on top of the chamber, sealed it in place with vacuum grease to protect from BABB/iDISCO spillage on my beloved optics.

Best wishes

Bertrand
--
Bertrand Vernay, PhD
Imaging Facility Manager
http://www.crm.ed.ac.uk/facilities/imaging
MRC Centre for Regenerative Medicine
SCRM Building
The University of Edinburgh
Edinburgh bioQuarter
5 Little France Drive
Edinburgh
EH16 4UU
 
Office: +0044 (0)131 651 9543