eGFP-mCherry FRET with three-filter method

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lechristophe lechristophe
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eGFP-mCherry FRET with three-filter method

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I would like to implement a simple FRET approach, using the three-filter
method (one for eGFP, one for mCherry, one for FRET). I know this has
been published for CFP/YFP pair, but do you know if it's possible with
eGFP/mCherry ? I could'nt find a commercially available eGFP/mCherry
FRET filter. What would be the spectral specs of such a filter set ?

Christophe Leterrier