Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalI would like to implement a simple FRET approach, using the three-filter
method (one for eGFP, one for mCherry, one for FRET). I know this has
been published for CFP/YFP pair, but do you know if it's possible with
eGFP/mCherry ? I could'nt find a commercially available eGFP/mCherry
FRET filter. What would be the spectral specs of such a filter set ?
Christophe Leterrier