electroporation of adherent cells

[Kevin Braeckmans]

Dear all,

 

Does anyone know of a commercially available instrument to do electroporation of an adherent cell monolayer on a cover slip? Preferably we would like to do the electroporation while imaging with the microscope.

 

Thanks for any suggestions,

 

Kind regards,

 

Kevin

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]

 

 

[Knecht, David]

BTX has several different adapters for their electroporator that will go into a petri dish.  If you put cells into a glass bottom petri dish and pulsed them it should do what you want.  I have not tried it myself so can't speak for the efficiency.   Dave

On Dec 22, 2008, at 12:26 PM, Kevin Braeckmans wrote:

Dear all,

 

Does anyone know of a commercially available instrument to do electroporation of an adherent cell monolayer on a cover slip? Preferably we would like to do the electroporation while imaging with the microscope.

 

Thanks for any suggestions,

 

Kind regards,

 

Kevin

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]

 

 

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)

[Noah Freedman]

(Commercial response)

Kevin

A scanning ion conductance microscope is optimised to image live adherent cell monolayers at high resolution (our ICnano achieves 20nm in XY, 5nm in Z).  In normal use, its operation avoids any mechanical interaction between the scanning probe (pipette) and the sample surface -- no contact and no applied force.  However, it has been used to porate individual target cells by one of two methods:  a) physically, by penetrating the plasma membrane using the pipette tip; and b) by delivering a chemical poration agent though the pipette local to the cell surface.  Scanning imaging can be carried out before and after poration.  Optical imaging using an inverted optical microscope can be performed throughout the process.

I hope this response is not deemed to be intrusive.

Regards

Noah

-- 

Noah Freedman

CEO

ionscope limited

A2 Ash House

Melbourn Science Park

Cambridge Road

Melbourn, SG8 6HB

UK

Tel: [phone]

Fax: [phone]

Mob: [phone]

Email: [hidden email]

Web: http://www.ionscope.com

On 22 Dec 2008, at 17:26, Kevin Braeckmans wrote:

Dear all,

 

Does anyone know of a commercially available instrument to do electroporation of an adherent cell monolayer on a cover slip? Preferably we would like to do the electroporation while imaging with the microscope.

 

Thanks for any suggestions,

 

Kind regards,

 

Kevin

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]