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Has anyone used these dyes? How specific are they for DNA? Molecular
probes makes them sound quite interesting for labeling nuclei:
https://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-stains.htmlBut there is very little information on them. I am interested in
alternatives to acridine orange for live cell imaging that are a little
more blue shifted. Right now I am using DAPI which works well, but the
diffusion time is a bit slow in thick tissues relative to acridine orange
and its a bit less DNA specific at higher concentrations. An acridine
variant that kept the high specificity and rapid diffusion of AO but was a
little blue shifted would be perfect.
Mike
Locked
