experience with CFM-3 high refractive index mountant and lipid stains

[jerie]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Listers,

2013 postings on CitiFluor™ CFM-3 sound rather positive (no commercial
interest), so I was wondering if anyone used it in combination with lipid
stains like Nile Red, Oil Red O, BODIPY...? My task is to image lipid
droplets aka lipid bodies in mouse tissues.

Would you share your experience? I asked @EMS and they guessed it could be
tricky because of their additives.

Any suggestions for alternatives that do not extract the lipids are very
welcome.

Thank you,

Jens
Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health
http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/

[Luis Farias]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Dr. Jens,

In our lab we have experienced good results using Osmium-Imidazole
protocols for Lipid Bodies for TEM, also there is a protocol for light
microscopy either.

Hope it helps,

regards,


Luis Henrique Seabra de Farias*, Msc.*
E-mail: [hidden email] <[hidden email]>
Universidade Federal do Pará
Instituto de Ciências Biológicas
Laboratório de Parasitologia/Laboratório de Biologia Estrutural
Telefone: 913201-7927/913201-8232

2015-05-12 14:42 GMT-03:00 jens rietdorf <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Listers,
>
> 2013 postings on CitiFluor™ CFM-3 sound rather positive (no commercial
> interest), so I was wondering if anyone used it in combination with lipid
> stains like Nile Red, Oil Red O, BODIPY...? My task is to image lipid
> droplets aka lipid bodies in mouse tissues.
>
> Would you share your experience? I asked @EMS and they guessed it could be
> tricky because of their additives.
>
> Any suggestions for alternatives that do not extract the lipids are very
> welcome.
>
> Thank you,
>
> Jens
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health
> http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/
>

... [show rest of quote]