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Dear Dr. Jens,
In our lab we have experienced good results using Osmium-Imidazole
protocols for Lipid Bodies for TEM, also there is a protocol for light
microscopy either.
Hope it helps,
regards,
Luis Henrique Seabra de Farias*, Msc.*
E-mail:
[hidden email] <
[hidden email]>
Universidade Federal do Pará
Instituto de Ciências Biológicas
Laboratório de Parasitologia/Laboratório de Biologia Estrutural
Telefone: 913201-7927/913201-8232
2015-05-12 14:42 GMT-03:00 jens rietdorf <
[hidden email]>:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Listers,
>
> 2013 postings on CitiFluor™ CFM-3 sound rather positive (no commercial
> interest), so I was wondering if anyone used it in combination with lipid
> stains like Nile Red, Oil Red O, BODIPY...? My task is to image lipid
> droplets aka lipid bodies in mouse tissues.
>
> Would you share your experience? I asked @EMS and they guessed it could be
> tricky because of their additives.
>
> Any suggestions for alternatives that do not extract the lipids are very
> welcome.
>
> Thank you,
>
> Jens
> Dr. Jens Rietdorf, visiting scientist @ center for technological
> development in health
>
http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/>
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