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Hi Randy,
a list of extinction coefficients is at
McNamara 2007 Fluorophore Data Tables
at
http://home.earthlink.net/~pubspectra/
Use Excel's search command (try full name, if that does not work, try
DEAC, or coumarin ... I found the E.c.'s below by searching for
coumarin). I've pasted a subset below.
Pacific Blue
3-carboxy-6,8-difluoro-7-hydroxycoumarin
29,500 (QY = 0.75)
Alexa Fluor 350 (Alexa350)
7-Amino-4-methyl-6-sulfocoumarin-3-acetic acid
15,500
AMCA and AMCA-X
(Coumarin)
19,000
7-methoxycoumarin-3-carboxylic
acid
26,000
7-diethylaminocoumarin-3-carboxylic
acid
56,000 = DEAC (though you wrote dimethyl [DMAC?], not diethyl
[DEAC])
D-AMCA
56,000
Hydroxy coumarin
(HYCO)
36,000
7-amino-4-methylcoumarin (row
4382)
18,000 (QY = 0.18) = DAMC?
6-chloro-7-hydroxy-coumarin-3-carbamide (row
4552)
44,000 (QY = 0.93)
Anyone looking for fluorescent protein data can find a few in the Excel
sheet at top, more in the
Data file at
McNamara G, Boswell C (2007) A Thousand Proteins of
Light: 15 Years of Advances in Fluorescent Proteins.
Modern Research and
Educational Topics in Microscopy (volume 3), in press.
http://www.formatex.org/microscopy3/
Downloads:
Word Doc Draft
Data
Enjoy,
George
At 09:28 AM 9/17/2007, you wrote:
Search the CONFOCAL archive
at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Does anyone know if there is (and where I can find) a comprehensive
list
of extinction coefficients for fluorescent compounds.
At the moment I am looking for the extinction coefficient of DEAC -
dimethylaminocoumarin dye.
Randy Learish
George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
[hidden email]
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305-243-8436 office
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