few seat still available ---Image processing workshop ABRF 2016 in sunny Florida

[Cole, Richard W (HEALTH)]

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Count, Measure and Showcase Your Images (February 20th 2016)

Small class size ensures ample interaction with the instructors and allows for personal solutions to be developed.  

Split afternoon sessions allow in depth exploration of preferred topics.

Organizers: Richard Cole, Wadsworth Center, Claire Brown, McGill University, Philip Hockberger, Northwestern, Teng Leong Chew, HHMI Janelia Research Campus

The workshop will be broken down into a morning session and two afternoon sessions. 

Session 1 (Morning)
The first session will feature presentations and hands-on:
. Image processing and analysis exercises for identifying and counting objects (e.g. noise reduction and filtering, edge detection, segmentation). 
. Measuring quantitative properties of objects (e.g. background and shading corrections, size, shape, intensity). FIJI and MetaMorph software will be used.
. Introduction to Multi-dimensional data: 4D (visualizing and publishing movies), true color imaging (human color perception, color space, tricks for printing and publishing in color)

Lunch will be provided

Session 2 (Choose One, run concurrently) (1st afternoon session)
2a. Color Imaging: Getting the most information out of true color images stained with H&E or for immunohistochemistry (control vs diseased tissues, machine learning software for color image analysis). Image Pro Premier software will be used.

2b. Co-localization: Pixel based measurements such as Pearson's and Mander's co-efficients, correlation co-efficients and object based co-localization (e.g. how many early endosomes contain my protein of interest). FIJI and Imaris software will be used.
Refreshment Break will be provided

Session 3 (Choose One, run concurrently) (2nd afternoon)
3a. 3D Image Deconvolution: Introduction to deconvolution, segmentation, counting and measuring objects in 3D data sets. FIJI and Autoquant software will be used?

3b. Time-Lapse Image Analysis: Exercises in how to create and present time series data for supplemental movies, seminar presentations and 3D graphs for publication. FIJI, Volocity and QuickTime

. Demo versions of the following software will be available prior the workshop:
. Media Cybernetics Image Pro Premier and AutoQuant (PC Only)
. Molecular Devices MetaMorph software (PC Only)
. Bitplane Imaris software (PC and Mac).

. All course materials will be available for download.
. We encourage registered participants to upload their own images and questions before the workshop.

Meeting website: https://conf.abrf.org/

Workshop website: https://conf.abrf.org/count-measure-showcase-images




Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
 
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York

120 New Scotland Avenue, Albany N.Y. 12208
518-474-7048 Phone
518-408-1730 Fax

Website www.wadsworth.org/cores/alm/index.htm