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I can tell you that amylose gives a very strong second harmonic signal, which could be useful. Reputedly amylopectin less so - there may therefore be a difference you could exploit there.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Agnes Höchstötter
Sent: Tuesday, 7 December 2010 11:39 AM
To:
[hidden email]
Subject: fluorescence-labeling of amylose and amylopectin (independently of each other)
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Hello,
I have been studying food science as a main subject at the Technical
University of Munich since 2006 and I'm searching for special fluorescent dyes/
fluorescently labeled antibodies or other possibility in order to stain amylose
and amylopectin in starch and/or cereal-based products for my diploma thesis.
I am concerned about being able to stain both amylose and amylopectin
individually (by this I mean that both should be coloured differently, so that I
can distinguish the two in the sample) under the CLSM.
Does anyone have any experience with this topic?
Thank you in advance!
Agnes Höchstötter
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