fluorescent beads-antibody conjugates
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Ekaterina Papusheva |
fluorescent beads-antibody conjugates
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Dear list,
I have a question concerning using antibody-conjugated fluorescent beads for long-time timelapse confocal imaging. we would like to conjugate fluorescent beads with (rabbit) antibodies, inject them into zebrafish embryos and track over time. The total number of images (z*T) will be about 20x50 =1000. If someone has experience with this kind of experiments, could you tell me: 1. what kind of beads do you use- we would like them to bleach not too fast and 2. could you share a good protocol for bead-antibody conjugation? if you have any tips about injecting beads into fish/xenopus embryo, that will also be great. Any tips will be very much appreciated! regards, Ekaterina -- postdoc Heisenberg lab Max-Planck-Institute for Molecular Cell Biology and Genetics Pfotenhauerstr.108 D-01307 Dresden Germany fon (lab) +49 351 2102712 |
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George McNamara |
Re: fluorescent beads-antibody conjugates
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Hi Ekaterina,
Brant Weinstein and lab have been injecting beads and things into zebrafish embryos for a long time. Check out http://uvo.nichd.nih.gov/science2.html and their papers. Scott Fraser's lab at Caltech may also have injected beads. Best wishes, George At 12:25 PM 7/30/2010, you wrote: >Dear list, > >I have a question concerning using antibody-conjugated fluorescent beads >for long-time timelapse confocal imaging. > >we would like to conjugate fluorescent beads with (rabbit) antibodies, >inject them into zebrafish embryos and track over time. The total number >of images (z*T) will be about 20x50 =1000. If someone has experience with >this kind of experiments, could you tell me: > >1. what kind of beads do you use- we would like them to bleach not too fast > >and > >2. could you share a good protocol for bead-antibody conjugation? > >if you have any tips about injecting beads into fish/xenopus embryo, that >will also be great. > >Any tips will be very much appreciated! > >regards, >Ekaterina > > > >-- >postdoc Heisenberg lab >Max-Planck-Institute for Molecular Cell Biology and Genetics >Pfotenhauerstr.108 >D-01307 Dresden >Germany >fon (lab) +49 351 2102712 George McNamara, Ph.D. Image Core Manager Analytical Imaging Core Facility University of Miami, Miller School of Medicine Miami, FL 33136 [hidden email] [hidden email] 305-243-8436 office http://www.sylvester.org/AICF (Analytical Imaging Core Facility) http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+ spectra .xlsx file is in the zip file) http://home.earthlink.net/~geomcnamara |
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Franklin, Peter |
Re: fluorescent beads-antibody conjugates
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In reply to this post by Ekaterina Papusheva
Jude, This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system. |
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Franklin, Peter |
Re: fluorescent beads-antibody conjugates
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In reply to this post by Ekaterina Papusheva
Sorry about my private comment! This email message, together with any attachments, is for the sole use of the intended recipient(s) and is the confidential information of Applied Precision Inc. If you are not the intended recipient, your review, use, disclosure, copying or dissemination of this email message or its attachments, or the information contained therein, is strictly prohibited. If you are not the intended recipient or if you think this email was sent to you in error, please notify the sender by reply email and delete this message and its attachments, as well as all copies, from your system. |
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