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I hope you can kindly help me with the following question
My goal is to make a microFRAP experiment at very high temporal resolution,
(MicroPoint Andor, and Spinning Disk Confocal, PerkinElmer)
what would be the BEST choice of existing fluorescent protein?.
I'd better choose a protein with very high brightness (eg LanYFP), and thus
improve temporal resolution, or choose a protein with a high photostability
(eg mNeonGreen).
or maybe I should choose one protein that will be easier to photobleach.
any other tips to be taken into consideration will be extremely grateful
thank you very much
regards
--
Jorge Toledo H.
Ph.D. student
Biomedical Neuroscience Institute (BNI)
Laboratory of cellular and molecular neurobiology (Couve-Lab)
Laboratory of Scientific Image Analysis (SCIAN-Lab)
Faculty of Medicine
University of Chile
www.couvelab.org | www.scian.cl
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