fluorophore data?

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mahogny mahogny
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fluorophore data?

Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan
Andrew Resnick Andrew Resnick
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Re: fluorophore data?

I use this table:

http://info.med.yale.edu/genetics/ward/tavi/FISHdyes2.html

which was apparently copied from Zeiss.

At 01:40 PM 5/11/2009, you wrote:

>Hello,
>I've been looking around for emission & absorption spectras for all
>common fluorophores but the data seems rather spread out. does
>anyone know of a compilation that legally could be included in an
>open source program?
>
>on a related note, can measured data be copyrighted?
>
>thanks
>/Johan

Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)
Cynara Y.Ko, Ph.D. Cynara Y.Ko, Ph.D.
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Re: fluorophore data?

In reply to this post by mahogny

Dear John,

 

Jackson ImmunoResearch Research labs’ Web site has some of the information.

 

http://www.jacksonimmuno.com/technical/DyLight.asp

 

http://www.jacksonimmuno.com/technical/f-fitc.asp

 

We also have text files: http://www.jacksonimmuno.com/technical/spectratext.asp.

 

Best regards,

Cynara

 


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Johan Henriksson
Sent: Monday, May 11, 2009 1:41 PM
To: [hidden email]
Subject: fluorophore data?

 

Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan

Boswell, Carl A - (cboswell) Boswell, Carl A - (cboswell)
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Re: fluorophore data?

In reply to this post by mahogny
Hi Johan,
This site should have what you are looking for:  http://www.mcb.arizona.edu/ipc/fret/.  I can send you the MS Access database on which it is based, if you wish.  It is my understanding that data cannot be copyrighted.
Cheers,
carl
 
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
----- Original Message -----
Sent: Monday, May 11, 2009 10:40 AM
Subject: fluorophore data?

Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan
Holly L. AARON Holly L. AARON
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Re: fluorophore data?

In reply to this post by mahogny
Hi, Johan -

A colleague of mine (Max Ulbrich) has an applet in which he has incorporated many different fluorophores (as well as filters, lasers, cameras, etc.) and their ex/em spectra from the manufacturers/producers. It's not all-inclusive, but if you send him suggestions, he will add to it!

You can view it here: http://www.online-spectra.com/applet.html

Best,
 Holly

Holly L Aaron
Molecular Imaging Center
Cancer Research Laboratory
447 Life Sciences Addition #2751
Berkeley, CA  94720-2751
510.642.2901
510.642.5741 FAX
[hidden email]
http://imaging.berkeley.edu
 
Johan Henriksson wrote:
Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan
Martin Wessendorf-2 Martin Wessendorf-2
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Re: fluorophore data?

In reply to this post by mahogny
Dear Johan--

George McNamara has links to a bunch of different sites:
http://home.earthlink.net/~geomcnamara/spectra_links.htm

Good luck!

Martin

Johan Henriksson wrote:

> Hello,
> I've been looking around for emission & absorption spectras for all
> common fluorophores but the data seems rather spread out. does anyone
> know of a compilation that legally could be included in an open source
> program?
>
> on a related note, can measured data be copyrighted?
>
> thanks
> /Johan
Anchall ............ Anchall ............
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Re: fluorophore data?

http://lamp3.tugraz.at/~fluorbase/

On Mon, May 11, 2009 at 9:02 PM, Martin Wessendorf <[hidden email]> wrote:
Dear Johan--

George McNamara has links to a bunch of different sites: http://home.earthlink.net/~geomcnamara/spectra_links.htm

Good luck!

Martin


Johan Henriksson wrote:
Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan

Guillermo Palchik Guillermo Palchik
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Re: fluorophore data?

In reply to this post by mahogny
http://fluorescence.nexus-solutions.net/frames6.htm



On May 11, 2009, at 1:40 PM, Johan Henriksson wrote:

> Hello,
> I've been looking around for emission & absorption spectras for all  
> common fluorophores but the data seems rather spread out. does  
> anyone know of a compilation that legally could be included in an  
> open source program?
>
> on a related note, can measured data be copyrighted?
>
> thanks
> /Johan

Guillermo Palchik
[hidden email]
Joost Willemse Joost Willemse
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Re: fluorophore data?

In reply to this post by mahogny
I recently downloaded instruview.

this makes use of the database found here
http://turmac13.chem.columbia.edu/fretview/test.dal

This can be imported and other fluorophores
added

Good luck with the search
Keith Morris Keith Morris
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Re: fluorophore data?

In reply to this post by mahogny

The Invitrogen spectra-viewer has superseded the Bio-Rad spectra viewer after Zeiss killed off Bio-Rad [UK]:

 

http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.reg.uk.html

 

It makes no comment on copyright, so probably an acknowledgement will suffice, particularly within PowerPoint presentations. You do have to know the analogues for some things, e.g DsRed. To copy image info use the keyboard PrintScreen button and paste into Photoshop to edit out what you need.

The Bio-Rad viewer is now reborn as the Zeiss Spectral Viewer

https://www.micro-shop.zeiss.com/us/us_en/spektral.php?cp_sid=&f=db
and it has DsRed

Also check out the flow cytometry site for their specific fluorescence surface type markers.

http://www.bdbiosciences.com/colors/fluorescence_spectrum_viewer

Disclaimer “This online tool is designed to aid customer understanding of important principles, dependencies, and relationships involved in selecting fluorochromes for multicolor flow cytometry. Users of this tool should ultimately rely on their own judgement when selecting fluorochromes for multicolor panels and experiments.”

Regards

 

Keith

 

PS found a nice link when looking for the Zeiss Spectra Viewer:

http://www.analytchem.tugraz.at/fluorophores/links.php

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  [hidden email]
Web-pages: http://www.well.ox.ac.uk/cytogenetics/


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Johan Henriksson
Sent: 11 May 2009 18:41
To: [hidden email]
Subject: fluorophore data?

 

Hello,
I've been looking around for emission & absorption spectras for all common fluorophores but the data seems rather spread out. does anyone know of a compilation that legally could be included in an open source program?

on a related note, can measured data be copyrighted?

thanks
/Johan

Keith Morris Keith Morris
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Re: fluorophore data?

In reply to this post by Guillermo Palchik
Sorry misread your question, missed the open source bit.

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  [hidden email]
Web-pages: http://www.well.ox.ac.uk/cytogenetics/
 
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Guillermo Palchik
Sent: 11 May 2009 21:07
To: [hidden email]
Subject: Re: fluorophore data?

http://fluorescence.nexus-solutions.net/frames6.htm



On May 11, 2009, at 1:40 PM, Johan Henriksson wrote:

> Hello,
> I've been looking around for emission & absorption spectras for all  
> common fluorophores but the data seems rather spread out. does  
> anyone know of a compilation that legally could be included in an  
> open source program?
>
> on a related note, can measured data be copyrighted?
>
> thanks
> /Johan

Guillermo Palchik
[hidden email]