forced user-initiative approach RE: oil protection for objectives on inverted scope

[Jennifer Clarke]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Hi Elke

This is a major issue in our facility, and several of our lenses are in a bad way (so much oil has accumulated into the springs of the lenses over the last 5 years that at some stage probably in the next 6 months we will need to be send them back to Germany for cleaning and re-aligning, this will be reasonably expensive and we are told the turn around time for this will approx 2 months (we are in Australia) - thats a long time for users to go without the lenses they need).

Hairbands are a great idea, and would stop oil running down the side of the lens - wish I'd implemented that years ago!
But nevertheless I believe users should not be lazy about removing oil

After years of firmly reminding users about the accumulative damage this buildup of oil within the lens springs does, and checking the lenses first and last thing of the day (I also work a different position with the same building so cant check between every user, and shouldnt have to), so I can never pinpoint a single user with absolute certainty (and of course they all strongly deny it was them),
we have recently implemented the a policy where each user is held individually accountable and we dont need to be able to check between every user.  Again I wish I'd enforced this years ago
This has been highly effective for some months, until this last week when I have had to temporarily suspend two users for 1 week each and warn their lab that next time anyone from their lab is negligent their whole lab will be suspended for up to a month.  They're not overly happy about it but it has got the message across finally.

Below is the policy I emailed to all users
(I cant believe we have to deal with supposedly intelligent scientists in this way, although of course is it only actually a few users who do the wrong thing)

Goodluck!
Jen


--
Dear Leica SP5 users at Flinders Microscopy

Unfortunately despite countless messages regarding oil on left on lenses and the problems this causes, it still seems to happen.
I am pretty fed up with having to explain this to people and this approach doesnt seem to have worked very well, so unfortunately it seems more direct action is necessary.

If oil is found left on any of the lenses, all users of the machine who have used it since I last checked the lenses (usually daily, but sometimes longer) can expect to be banned from using the machine for a period of time. Particularly bad instances and repeat offenders may have their entire lab being banned from using the machine for a period of time.

So
- take the initiative to check all lenses before you start your work.  If you find oil left on an oil lens before you start please let me know, and tell me which lens is involved.  If I'm not available at the time then TAKE THE INITIATIVE to wipe the excess oil off with a clean tissue, and please leave me a message by email, including which lens this involved.

- also, as per email last week, TAKE THE INITIATIVE to note who is booked on after you, so if you leave the equipment on for someone who subsequently cancels we can track who was responsible.

Doing these 2 things will prevent you having your access banned if it wasnt your fault, and help ensure that everyone takes responsibility for the care of the machine (or risk having their access removed/restricted).

If you do not understand why oil should not be left on the lenses then please refer to my previous detailed emails on the topic.  Oil should neither be allowed to run down the side of any lens, nor should a visible amount be left on the top of the lens.  If this is still not clear come and see me.

Kind regards
Jen
--
Jennifer Clarke BSc (Hons) PhD
Research Associate, Anatomy and Histology
Centre for Neuroscience, School of Medicine
&
Facility Manager, Optical Microscopy Suite, Flinders Microscopy
(training and assistance available on Mondays only)

Flinders University
GPO Box 2100, Adelaide 5001
Phone: [phone] 8204 6637
Email: [hidden email]
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Eric Marino [[hidden email]]
Sent: Wednesday, 27 July 2011 7:24 AM
To: [hidden email]
Subject: Re: oil protection for objectives on inverted scope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

We use felt rings that have a snug fit. Depending on usage, each ring lasts for over a month, I've been using them for 4 years now without a problem. I also stress to the users that they wipe off the oil and re-apply it every time they change specimens, that way you prevent build-up.

NO COMMERCIAL INTEREST:

Felt Objective Ring Supplier
Southeastern Felt & Supply Corp
Concord, NC 28025

f-1 white wool felt , 30 mm OD x 2.5 mm ID x 1/8”

I think that we paid about 0.37/each

On Jul 26, 2011, at 4:58 PM, Z.J. Zhang wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> The Aquastop, if I am not mistaken, is mainly for preventing spill of liquid medium. It does not work well with oil.
>
> Zhaojie
>
> Zhaojie Zhang, Ph. D.
> Director, Jenkins Microscopy Facility
> University of Wyoming
> Laramie, WY 82071
> PHONE: [phone]
> FAX: [phone]
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Damir Sudar
> Sent: Tuesday, July 26, 2011 1:28 PM
> To: [hidden email]
> Subject: Re: oil protection for objectives on inverted scope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> another option: Not as cost-effective as the ones on your list but
> functionally quite effective is Zeiss's Aquastop. See:
> http://www.zeiss.de/C12567BE00472A5C/ContainerTitel/Axio_Observer/$File/sicherer2_en.html
>
> No commercial interest, just a customer.
> - Damir
>
> On 7/26/2011 11:47 AM, Elke Kuster-Schock wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hello,
>>
>> I was hoping for input on how to protect the objectives on an inverted scope
>> from oil running down the sides, and ultimately into the objective.
>>
>> We are running a core facility with many users, and the obvious solution (use
>> less oil) doesn't seem to be obvious to some of them.
>> Especially on our Zeiss LSM 510s, due to the design of the objectives, we see
>> that oil finds its way into the objectives (and in one extreme case, even back
>> out of the bottom), and we are wasting time and resources to clean and repair
>> them.
>>
>> I've looked around the web, and brainstormed with colleagues, and found the
>> following:
>>
>> 1 - Cut a finger off an examination glove, cut a small hole at the end, and slip
>> over entire objective.
>> Concerns: Does the glove fit tight enough so that oil doesn't get sucked under
>> it by capillary action? Will the oil degrade latex/nitrile?
>>
>> 2 - Put an O-ring around objective as an "oil dam."
>> Concerns: Similar to 1, worries about tightness of seal, and stability of
>> material. What kind of O-ring should I look for? Simple rubber band?
>>
>> 3 - Create a drip collar (similar to wine bottle collar) by wrapping layers of
>> lens paper or other tissue around objective and securing with rubber band.
>> Concerns: Similar to 1 and 2.
>>
>> I would appreciate if people on the forum could give feedback on methods that
>> have worked for them.
>> Thanks,
>> Elke
>>
>>
>> ----------------------------------------------------------------
>> Elke Küster-Schöck
>> CIAN (Cell Imaging and Analysis Network)
>> Proteomics&  Genomics Coordinator, Microscopy Associate
>> McGill University, Montreal QC, Canada
>
> --
> Damir Sudar - Staff Scientist and Deputy for Technology
> Lawrence Berkeley Laboratory / Life Sciences Division
> One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
> T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
> WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html
>

Eric Marino
Senior Imaging Specialist
Immune Disease Institute
Harvard Medical School
200 Longwood Ave
WAB 133D
Boston, MA 02115
Lab: 617 713-8885
Cell: 617 913-9647
[hidden email]

[George McNamara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Jen,

Your message to your users seems (to me) to leave room for more problems
- you do not state that users should only use lens paper, not kimwipes,
kleenez, sandpaper, etc, when cleaning lenses.

Do you have a sign-in sheet of paper, a computer login, University ID
scan card access reader (for PC access to the instrument)?    My SP5's
are in a room without a scan card reader on the door, and even if I did,
there is possibility of the door being left open or someone else letting
them in. I am still using written sign-in, and some users save their
Leica SP5 LIF files directly to portable drive or server, so I do not
have an easy way of verifying all user access.

Sincerely,

George
p.s. one of the Leica field service engineers told me the SP5 software
(LAS AF) has a database that tracks every image acquired. If anyone on
the listserv knows how to access that, could you please post it on the
listserv? I also manage Zeiss LSM710 (ZEN 2009) and 510 (AIM) so if
there is image tracking on those I would love to learn how to access it.
Speaking of LSM510 - sale of the 510 I manage fell through - I have not
had time to contact the other folks interested in it - feel free to take
the initiative and contact me offline if you are interested in
purchasing ours. [hidden email]

On 7/26/2011 9:48 PM, Jen Clarke wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Elke
>
> This is a major issue in our facility, and several of our lenses are in a bad way (so much oil has accumulated into the springs of the lenses over the last 5 years that at some stage probably in the next 6 months we will need to be send them back to Germany for cleaning and re-aligning, this will be reasonably expensive and we are told the turn around time for this will approx 2 months (we are in Australia) - thats a long time for users to go without the lenses they need).
>
> Hairbands are a great idea, and would stop oil running down the side of the lens - wish I'd implemented that years ago!
> But nevertheless I believe users should not be lazy about removing oil
>
> After years of firmly reminding users about the accumulative damage this buildup of oil within the lens springs does, and checking the lenses first and last thing of the day (I also work a different position with the same building so cant check between every user, and shouldnt have to), so I can never pinpoint a single user with absolute certainty (and of course they all strongly deny it was them),
> we have recently implemented the a policy where each user is held individually accountable and we dont need to be able to check between every user.  Again I wish I'd enforced this years ago
> This has been highly effective for some months, until this last week when I have had to temporarily suspend two users for 1 week each and warn their lab that next time anyone from their lab is negligent their whole lab will be suspended for up to a month.  They're not overly happy about it but it has got the message across finally.
>
> Below is the policy I emailed to all users
> (I cant believe we have to deal with supposedly intelligent scientists in this way, although of course is it only actually a few users who do the wrong thing)
>
> Goodluck!
> Jen
>
>
> --
> Dear Leica SP5 users at Flinders Microscopy
>
> Unfortunately despite countless messages regarding oil on left on lenses and the problems this causes, it still seems to happen.
> I am pretty fed up with having to explain this to people and this approach doesnt seem to have worked very well, so unfortunately it seems more direct action is necessary.
>
> If oil is found left on any of the lenses, all users of the machine who have used it since I last checked the lenses (usually daily, but sometimes longer) can expect to be banned from using the machine for a period of time. Particularly bad instances and repeat offenders may have their entire lab being banned from using the machine for a period of time.
>
> So
> - take the initiative to check all lenses before you start your work.  If you find oil left on an oil lens before you start please let me know, and tell me which lens is involved.  If I'm not available at the time then TAKE THE INITIATIVE to wipe the excess oil off with a clean tissue, and please leave me a message by email, including which lens this involved.
>
> - also, as per email last week, TAKE THE INITIATIVE to note who is booked on after you, so if you leave the equipment on for someone who subsequently cancels we can track who was responsible.
>
> Doing these 2 things will prevent you having your access banned if it wasnt your fault, and help ensure that everyone takes responsibility for the care of the machine (or risk having their access removed/restricted).
>
> If you do not understand why oil should not be left on the lenses then please refer to my previous detailed emails on the topic.  Oil should neither be allowed to run down the side of any lens, nor should a visible amount be left on the top of the lens.  If this is still not clear come and see me.
>
> Kind regards
> Jen
> --
> Jennifer Clarke BSc (Hons) PhD
> Research Associate, Anatomy and Histology
> Centre for Neuroscience, School of Medicine
> &
> Facility Manager, Optical Microscopy Suite, Flinders Microscopy
> (training and assistance available on Mondays only)
>
> Flinders University
> GPO Box 2100, Adelaide 5001
> Phone: [phone] 8204 6637
> Email: [hidden email]
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On Behalf Of Eric Marino [[hidden email]]
> Sent: Wednesday, 27 July 2011 7:24 AM
> To: [hidden email]
> Subject: Re: oil protection for objectives on inverted scope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We use felt rings that have a snug fit. Depending on usage, each ring lasts for over a month, I've been using them for 4 years now without a problem. I also stress to the users that they wipe off the oil and re-apply it every time they change specimens, that way you prevent build-up.
>
> NO COMMERCIAL INTEREST:
>
> Felt Objective Ring Supplier
> Southeastern Felt&  Supply Corp
> Concord, NC 28025
>
> f-1 white wool felt , 30 mm OD x 2.5 mm ID x 1/8”
>
> I think that we paid about 0.37/each
>
> On Jul 26, 2011, at 4:58 PM, Z.J. Zhang wrote:
>
>    
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> The Aquastop, if I am not mistaken, is mainly for preventing spill of liquid medium. It does not work well with oil.
>>
>> Zhaojie
>>
>> Zhaojie Zhang, Ph. D.
>> Director, Jenkins Microscopy Facility
>> University of Wyoming
>> Laramie, WY 82071
>> PHONE: [phone]
>> FAX: [phone]
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Damir Sudar
>> Sent: Tuesday, July 26, 2011 1:28 PM
>> To: [hidden email]
>> Subject: Re: oil protection for objectives on inverted scope
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> another option: Not as cost-effective as the ones on your list but
>> functionally quite effective is Zeiss's Aquastop. See:
>> http://www.zeiss.de/C12567BE00472A5C/ContainerTitel/Axio_Observer/$File/sicherer2_en.html
>>
>> No commercial interest, just a customer.
>> - Damir
>>
>> On 7/26/2011 11:47 AM, Elke Kuster-Schock wrote:
>>      
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hello,
>>>
>>> I was hoping for input on how to protect the objectives on an inverted scope
>>> from oil running down the sides, and ultimately into the objective.
>>>
>>> We are running a core facility with many users, and the obvious solution (use
>>> less oil) doesn't seem to be obvious to some of them.
>>> Especially on our Zeiss LSM 510s, due to the design of the objectives, we see
>>> that oil finds its way into the objectives (and in one extreme case, even back
>>> out of the bottom), and we are wasting time and resources to clean and repair
>>> them.
>>>
>>> I've looked around the web, and brainstormed with colleagues, and found the
>>> following:
>>>
>>> 1 - Cut a finger off an examination glove, cut a small hole at the end, and slip
>>> over entire objective.
>>> Concerns: Does the glove fit tight enough so that oil doesn't get sucked under
>>> it by capillary action? Will the oil degrade latex/nitrile?
>>>
>>> 2 - Put an O-ring around objective as an "oil dam."
>>> Concerns: Similar to 1, worries about tightness of seal, and stability of
>>> material. What kind of O-ring should I look for? Simple rubber band?
>>>
>>> 3 - Create a drip collar (similar to wine bottle collar) by wrapping layers of
>>> lens paper or other tissue around objective and securing with rubber band.
>>> Concerns: Similar to 1 and 2.
>>>
>>> I would appreciate if people on the forum could give feedback on methods that
>>> have worked for them.
>>> Thanks,
>>> Elke
>>>
>>>
>>> ----------------------------------------------------------------
>>> Elke Küster-Schöck
>>> CIAN (Cell Imaging and Analysis Network)
>>> Proteomics&   Genomics Coordinator, Microscopy Associate
>>> McGill University, Montreal QC, Canada
>>>        
>> --
>> Damir Sudar - Staff Scientist and Deputy for Technology
>> Lawrence Berkeley Laboratory / Life Sciences Division
>> One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
>> T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
>> WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html
>>
>>      
>
> Eric Marino
> Senior Imaging Specialist
> Immune Disease Institute
> Harvard Medical School
> 200 Longwood Ave
> WAB 133D
> Boston, MA 02115
> Lab: 617 713-8885
> Cell: 617 913-9647
> [hidden email]
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[Guy Cox-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

George,

           Years back we used to work on a paper sign-in system.  For absolute accuracy it's unbeatable.  But the labour involved in then inputting it to a billing/logging system is too much.  We now use a computer booking/logging system with no 'lock-out' provisions, and it works - largely, I suppose, because the usage is heavy enough that someone who tries to cheat the system will be caught out in pretty short order.

                                                Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Wednesday, 27 July 2011 8:51 PM
To: [hidden email]
Subject: Re: forced user-initiative approach RE: oil protection for objectives on inverted scope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Jen,

Your message to your users seems (to me) to leave room for more problems
- you do not state that users should only use lens paper, not kimwipes,
kleenez, sandpaper, etc, when cleaning lenses.

Do you have a sign-in sheet of paper, a computer login, University ID
scan card access reader (for PC access to the instrument)?    My SP5's
are in a room without a scan card reader on the door, and even if I did,
there is possibility of the door being left open or someone else letting
them in. I am still using written sign-in, and some users save their
Leica SP5 LIF files directly to portable drive or server, so I do not
have an easy way of verifying all user access.

Sincerely,

George
p.s. one of the Leica field service engineers told me the SP5 software
(LAS AF) has a database that tracks every image acquired. If anyone on
the listserv knows how to access that, could you please post it on the
listserv? I also manage Zeiss LSM710 (ZEN 2009) and 510 (AIM) so if
there is image tracking on those I would love to learn how to access it.
Speaking of LSM510 - sale of the 510 I manage fell through - I have not
had time to contact the other folks interested in it - feel free to take
the initiative and contact me offline if you are interested in
purchasing ours. [hidden email]

On 7/26/2011 9:48 PM, Jen Clarke wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Elke
>
> This is a major issue in our facility, and several of our lenses are in a bad way (so much oil has accumulated into the springs of the lenses over the last 5 years that at some stage probably in the next 6 months we will need to be send them back to Germany for cleaning and re-aligning, this will be reasonably expensive and we are told the turn around time for this will approx 2 months (we are in Australia) - thats a long time for users to go without the lenses they need).
>
> Hairbands are a great idea, and would stop oil running down the side of the lens - wish I'd implemented that years ago!
> But nevertheless I believe users should not be lazy about removing oil
>
> After years of firmly reminding users about the accumulative damage this buildup of oil within the lens springs does, and checking the lenses first and last thing of the day (I also work a different position with the same building so cant check between every user, and shouldnt have to), so I can never pinpoint a single user with absolute certainty (and of course they all strongly deny it was them),
> we have recently implemented the a policy where each user is held individually accountable and we dont need to be able to check between every user.  Again I wish I'd enforced this years ago
> This has been highly effective for some months, until this last week when I have had to temporarily suspend two users for 1 week each and warn their lab that next time anyone from their lab is negligent their whole lab will be suspended for up to a month.  They're not overly happy about it but it has got the message across finally.
>
> Below is the policy I emailed to all users
> (I cant believe we have to deal with supposedly intelligent scientists in this way, although of course is it only actually a few users who do the wrong thing)
>
> Goodluck!
> Jen
>
>
> --
> Dear Leica SP5 users at Flinders Microscopy
>
> Unfortunately despite countless messages regarding oil on left on lenses and the problems this causes, it still seems to happen.
> I am pretty fed up with having to explain this to people and this approach doesnt seem to have worked very well, so unfortunately it seems more direct action is necessary.
>
> If oil is found left on any of the lenses, all users of the machine who have used it since I last checked the lenses (usually daily, but sometimes longer) can expect to be banned from using the machine for a period of time. Particularly bad instances and repeat offenders may have their entire lab being banned from using the machine for a period of time.
>
> So
> - take the initiative to check all lenses before you start your work.  If you find oil left on an oil lens before you start please let me know, and tell me which lens is involved.  If I'm not available at the time then TAKE THE INITIATIVE to wipe the excess oil off with a clean tissue, and please leave me a message by email, including which lens this involved.
>
> - also, as per email last week, TAKE THE INITIATIVE to note who is booked on after you, so if you leave the equipment on for someone who subsequently cancels we can track who was responsible.
>
> Doing these 2 things will prevent you having your access banned if it wasnt your fault, and help ensure that everyone takes responsibility for the care of the machine (or risk having their access removed/restricted).
>
> If you do not understand why oil should not be left on the lenses then please refer to my previous detailed emails on the topic.  Oil should neither be allowed to run down the side of any lens, nor should a visible amount be left on the top of the lens.  If this is still not clear come and see me.
>
> Kind regards
> Jen
> --
> Jennifer Clarke BSc (Hons) PhD
> Research Associate, Anatomy and Histology
> Centre for Neuroscience, School of Medicine
> &
> Facility Manager, Optical Microscopy Suite, Flinders Microscopy
> (training and assistance available on Mondays only)
>
> Flinders University
> GPO Box 2100, Adelaide 5001
> Phone: [phone] 8204 6637
> Email: [hidden email]
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On Behalf Of Eric Marino [[hidden email]]
> Sent: Wednesday, 27 July 2011 7:24 AM
> To: [hidden email]
> Subject: Re: oil protection for objectives on inverted scope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We use felt rings that have a snug fit. Depending on usage, each ring lasts for over a month, I've been using them for 4 years now without a problem. I also stress to the users that they wipe off the oil and re-apply it every time they change specimens, that way you prevent build-up.
>
> NO COMMERCIAL INTEREST:
>
> Felt Objective Ring Supplier
> Southeastern Felt&  Supply Corp
> Concord, NC 28025
>
> f-1 white wool felt , 30 mm OD x 2.5 mm ID x 1/8"
>
> I think that we paid about 0.37/each
>
> On Jul 26, 2011, at 4:58 PM, Z.J. Zhang wrote:
>
>    
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> The Aquastop, if I am not mistaken, is mainly for preventing spill of liquid medium. It does not work well with oil.
>>
>> Zhaojie
>>
>> Zhaojie Zhang, Ph. D.
>> Director, Jenkins Microscopy Facility
>> University of Wyoming
>> Laramie, WY 82071
>> PHONE: [phone]
>> FAX: [phone]
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Damir Sudar
>> Sent: Tuesday, July 26, 2011 1:28 PM
>> To: [hidden email]
>> Subject: Re: oil protection for objectives on inverted scope
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> another option: Not as cost-effective as the ones on your list but
>> functionally quite effective is Zeiss's Aquastop. See:
>> http://www.zeiss.de/C12567BE00472A5C/ContainerTitel/Axio_Observer/$File/sicherer2_en.html
>>
>> No commercial interest, just a customer.
>> - Damir
>>
>> On 7/26/2011 11:47 AM, Elke Kuster-Schock wrote:
>>      
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hello,
>>>
>>> I was hoping for input on how to protect the objectives on an inverted scope
>>> from oil running down the sides, and ultimately into the objective.
>>>
>>> We are running a core facility with many users, and the obvious solution (use
>>> less oil) doesn't seem to be obvious to some of them.
>>> Especially on our Zeiss LSM 510s, due to the design of the objectives, we see
>>> that oil finds its way into the objectives (and in one extreme case, even back
>>> out of the bottom), and we are wasting time and resources to clean and repair
>>> them.
>>>
>>> I've looked around the web, and brainstormed with colleagues, and found the
>>> following:
>>>
>>> 1 - Cut a finger off an examination glove, cut a small hole at the end, and slip
>>> over entire objective.
>>> Concerns: Does the glove fit tight enough so that oil doesn't get sucked under
>>> it by capillary action? Will the oil degrade latex/nitrile?
>>>
>>> 2 - Put an O-ring around objective as an "oil dam."
>>> Concerns: Similar to 1, worries about tightness of seal, and stability of
>>> material. What kind of O-ring should I look for? Simple rubber band?
>>>
>>> 3 - Create a drip collar (similar to wine bottle collar) by wrapping layers of
>>> lens paper or other tissue around objective and securing with rubber band.
>>> Concerns: Similar to 1 and 2.
>>>
>>> I would appreciate if people on the forum could give feedback on methods that
>>> have worked for them.
>>> Thanks,
>>> Elke
>>>
>>>
>>> ----------------------------------------------------------------
>>> Elke Küster-Schöck
>>> CIAN (Cell Imaging and Analysis Network)
>>> Proteomics&   Genomics Coordinator, Microscopy Associate
>>> McGill University, Montreal QC, Canada
>>>        
>> --
>> Damir Sudar - Staff Scientist and Deputy for Technology
>> Lawrence Berkeley Laboratory / Life Sciences Division
>> One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
>> T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
>> WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html
>>
>>      
>
> Eric Marino
> Senior Imaging Specialist
> Immune Disease Institute
> Harvard Medical School
> 200 Longwood Ave
> WAB 133D
> Boston, MA 02115
> Lab: 617 713-8885
> Cell: 617 913-9647
> [hidden email]
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[W. Chan]

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*****

Hi George

You can run an error report and grab the archive. I think it is drive:\Program Files\Leica Microsystems CMS GmbH.zip\Leica Microsystems CMS GmbH\LAS AF\BIN\SendErrorReport.exe.

E.g., if you want user logon time, parse through 002_LASAFLogfile.log and search for "Start LAS AF".  Almost everything the users and the machine did are recorded in one of the 42 log files there.  The file SaveErrorReport.log in the archive has the data sources.  Enjoy.

On Wed, 27 Jul 2011, George McNamara wrote:

> p.s. one of the Leica field service engineers told me the SP5 software (LAS
> AF) has a database that tracks every image acquired. If anyone on the listserv
> knows how to access that, could you please post it on the listserv?

--
Pang (Wai Pang Chan, [hidden email], PAB A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)

[Jennifer Clarke]

Thanks for all reponses
and thankyou very much for the info for obtaining the archive of what users have done (much quicker that going through their lif files (if left on machine) to see who used what lens last)
Cheers
Jen
--
Jennifer Clarke BSc (Hons) PhD
Research Associate, Anatomy and Histology
Centre for Neuroscience, School of Medicine
&
Facility Manager, Optical Microscopy Suite, Flinders Microscopy
(training and assistance available on Mondays only)

Flinders University
GPO Box 2100, Adelaide 5001
Phone: [phone] 8204 6637
Email: [hidden email]
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of W. Chan [[hidden email]]
Sent: Thursday, 28 July 2011 6:29 AM
To: [hidden email]
Subject: SP5 log (Re: [CONFOCALMICROSCOPY] forced user-initiative approach RE: oil protection for objectives on inverted scope)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi George

You can run an error report and grab the archive. I think it is drive:\Program Files\Leica Microsystems CMS GmbH.zip\Leica Microsystems CMS GmbH\LAS AF\BIN\SendErrorReport.exe.

E.g., if you want user logon time, parse through 002_LASAFLogfile.log and search for "Start LAS AF".  Almost everything the users and the machine did are recorded in one of the 42 log files there.  The file SaveErrorReport.log in the archive has the data sources.  Enjoy.

On Wed, 27 Jul 2011, George McNamara wrote:

> p.s. one of the Leica field service engineers told me the SP5 software (LAS
> AF) has a database that tracks every image acquired. If anyone on the listserv
> knows how to access that, could you please post it on the listserv?

--
Pang (Wai Pang Chan, [hidden email], PAB A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)