growing mouse lung cancer cells on glass

[Adams,Henry P]

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Greetings,

Does anyone have experience growing mouse lung cancer cells on glass cover slips?  I have an user who is having difficulty getting these cells to stay attached and grow.
I believe he has tried uncoated and poly-lysine coated coverslips.  Any suggestions would be immensely appreciated by him.

Hank Adams
Genetics Department
Rm S14.8316A
U.T. M.D.Anderson Cancer Center
Houston, Tx 77030

[Chris Guerin]

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Hi Hank:

        Our users have a lot of success with Cel-Tak from BD. We haven't found anything yet that doesn't stick to it and haven't noticed any negative effects (i.e. increased background).

Good luck,

Chris Guérin
Leader, Microscopy Core
Dept. of Molecular Biomedical Research
Flanders Institute of Biotechnology, Univ. Ghent

P.S. No commercial interest, just a satisfied customer.

[Adams,Henry P]

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Chris,

I have a question which maybe is foolish, but can one use Cell-Tak for mobility assays?

Hank

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Chris Guerin
Sent: Wednesday, March 23, 2011 5:48 AM
To: [hidden email]
Subject: Re: growing mouse lung cancer cells on glass

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Hi Hank:

        Our users have a lot of success with Cel-Tak from BD. We haven't found anything yet that doesn't stick to it and haven't noticed any negative effects (i.e. increased background).

Good luck,

Chris Guérin
Leader, Microscopy Core
Dept. of Molecular Biomedical Research
Flanders Institute of Biotechnology, Univ. Ghent

P.S. No commercial interest, just a satisfied customer.