help with Zeiss NDD

[robert_duv]

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Hi

I have inherited parts of a 2-photons microscope. I have the 2 P laser (Coherent) and a Zeiss Axioskop 2 FS mot. (I'm missing the LSM 510 scanhead, computer and control box). I need to build the scanhead and am hoping to run it with ScanImage. Does anyone know how to control and acquire data from the Zeiss NDDs, if possible including the motorized non-descanned detection kit and the detection module PMT?

I'm happy to provide more details if that helps. Any advice is also welcome.
Thanks
Robert

[Craig Brideau]

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Without the scanhead and control box you are pretty much starting from
scratch.  Unless you can get Zeiss to tell you how the NDDs work they won't
be a whole lot of use to you.  You might be able to strip them right down to
the PMTs and get the signals directly.  I'd recommend trying to get the
missing pieces you need to turn it into a complete Zeiss (maybe somebody has
traded in an LSM 510 you can get for cheap?) or scrap the NDDs and buy a 2
photon conversion kit from thorlabs or somebody.  Since you need the
scanhead, controller, and software you actually need quite a bit of
hardware.

Craig




On Mon, Dec 13, 2010 at 6:40 PM, Robert Duvoisin <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I have inherited parts of a 2-photons microscope. I have the 2 P laser
> (Coherent) and a Zeiss Axioskop 2 FS mot. (I'm missing the LSM 510 scanhead,
> computer and control box). I need to build the scanhead and am hoping to run
> it with ScanImage. Does anyone know how to control and acquire data from the
> Zeiss NDDs, if possible including the motorized non-descanned detection kit
> and the detection module PMT?
>
> I'm happy to provide more details if that helps. Any advice is also
> welcome.
> Thanks
> Robert
>

[Guy Cox-2]

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Craig's advice seems unduly pessimistic.  Zeiss NDDs will just be boxes
with one or more PMTs in them, with or without preamps.  Connecting them
up should be no harder than using any other detectors with a 'do it
yourself' scanhead.  It would probably be handy to find someone with a
similar Zeiss system so that you can hook a scope up and find out what
their output is in normal operation.  There are any number of people who
have built their own 2P only systems and could offer advice  - Paul
Herzmark at UC Berkeley is one I know, and he's very helpful.  (He and I
both seem to have become itinerant globe-trotting microscopy educators!)


 

 
Guy

 

Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm
<http://www.guycox.com/optical.htm>

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Australian Centre for Microscopy & Microanalysis,

Madsen Building F09, University of Sydney, NSW 2006

 

Phone [phone]     Fax [phone]

             Mobile [phone]

______________________________________________

      http://www.guycox.net <http://www.guycox.net>

 

 

From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Craig Brideau
Sent: Tuesday, 14 December 2010 12:59 PM
To: [hidden email]
Subject: Re: help with Zeiss NDD

 

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Without the scanhead and control box you are pretty much starting from
scratch.  Unless you can get Zeiss to tell you how the NDDs work they
won't
be a whole lot of use to you.  You might be able to strip them right
down to
the PMTs and get the signals directly.  I'd recommend trying to get the
missing pieces you need to turn it into a complete Zeiss (maybe somebody
has
traded in an LSM 510 you can get for cheap?) or scrap the NDDs and buy a
2
photon conversion kit from thorlabs or somebody.  Since you need the
scanhead, controller, and software you actually need quite a bit of
hardware.

Craig




On Mon, Dec 13, 2010 at 6:40 PM, Robert Duvoisin <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I have inherited parts of a 2-photons microscope. I have the 2 P laser
> (Coherent) and a Zeiss Axioskop 2 FS mot. (I'm missing the LSM 510
scanhead,
> computer and control box). I need to build the scanhead and am hoping
to run
> it with ScanImage. Does anyone know how to control and acquire data
from the
> Zeiss NDDs, if possible including the motorized non-descanned
detection kit
> and the detection module PMT?
>
> I'm happy to provide more details if that helps. Any advice is also
> welcome.
> Thanks
> Robert
>

________________________________

No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1170 / Virus Database: 426/3313 - Release Date: 12/13/10

[Craig Brideau]

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Heh.  I've had to rip apart existing systems before like this and
manufacturers seem to go out of their way to make things difficult to
access, hence my pessimism.  Still though, Guy's right; if you can find the
right connections you should be OK, but as he says it might be a good idea
to check that they work first if you can find another Zeiss to hook them up
to for a check.  Also, do you have a power supply for the PMTs?  There are a
few companies that make them, but you will have to find ones compatible with
whatever is in the NDD box.

Craig


On Tue, Dec 14, 2010 at 12:42 AM, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Craig's advice seems unduly pessimistic.  Zeiss NDDs will just be boxes
> with one or more PMTs in them, with or without preamps.  Connecting them
> up should be no harder than using any other detectors with a 'do it
> yourself' scanhead.  It would probably be handy to find someone with a
> similar Zeiss system so that you can hook a scope up and find out what
> their output is in normal operation.  There are any number of people who
> have built their own 2P only systems and could offer advice  - Paul
> Herzmark at UC Berkeley is one I know, and he's very helpful.  (He and I
> both seem to have become itinerant globe-trotting microscopy educators!)
>
>
>
>
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
>
> by Guy Cox    CRC Press / Taylor & Francis
>
>     http://www.guycox.com/optical.htm
> <http://www.guycox.com/optical.htm>
>
> ______________________________________________
>
> Associate Professor Guy Cox, MA, DPhil(Oxon)
>
> Australian Centre for Microscopy & Microanalysis,
>
> Madsen Building F09, University of Sydney, NSW 2006
>
>
>
> Phone [phone]     Fax [phone]
>
>             Mobile [phone]
>
> ______________________________________________
>
>      http://www.guycox.net <http://www.guycox.net>
>
>
>
>
>
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Tuesday, 14 December 2010 12:59 PM
> To: [hidden email]
> Subject: Re: help with Zeiss NDD
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Without the scanhead and control box you are pretty much starting from
> scratch.  Unless you can get Zeiss to tell you how the NDDs work they
> won't
> be a whole lot of use to you.  You might be able to strip them right
> down to
> the PMTs and get the signals directly.  I'd recommend trying to get the
> missing pieces you need to turn it into a complete Zeiss (maybe somebody
> has
> traded in an LSM 510 you can get for cheap?) or scrap the NDDs and buy a
> 2
> photon conversion kit from thorlabs or somebody.  Since you need the
> scanhead, controller, and software you actually need quite a bit of
> hardware.
>
> Craig
>
>
>
>
> On Mon, Dec 13, 2010 at 6:40 PM, Robert Duvoisin <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi
> >
> > I have inherited parts of a 2-photons microscope. I have the 2 P laser
> > (Coherent) and a Zeiss Axioskop 2 FS mot. (I'm missing the LSM 510
> scanhead,
> > computer and control box). I need to build the scanhead and am hoping
> to run
> > it with ScanImage. Does anyone know how to control and acquire data
> from the
> > Zeiss NDDs, if possible including the motorized non-descanned
> detection kit
> > and the detection module PMT?
> >
> > I'm happy to provide more details if that helps. Any advice is also
> > welcome.
> > Thanks
> > Robert
> >
>
> ________________________________
>
> No virus found in this message.
> Checked by AVG - www.avg.com
> Version: 10.0.1170 / Virus Database: 426/3313 - Release Date: 12/13/10
>

[Paul Herzmark]

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*****

Thanks Guy for the testimonial!  And yes, I would be happy to help!

Paul

Paul Herzmark
Specialist
[hidden email]

Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA  94720-3200
[phone]
[phone] fax


 Guy Cox <[hidden email]> wrote:

> *****
>
> Craig's advice seems unduly pessimistic.  Zeiss NDDs will just be boxes
> with one or more PMTs in them, with or without preamps.  Connecting them
> up should be no harder than using any other detectors with a 'do it
> yourself' scanhead.  It would probably be handy to find someone with a
> similar Zeiss system so that you can hook a scope up and find out what
> their output is in normal operation.  There are any number of people who
> have built their own 2P only systems and could offer advice  - Paul
> Herzmark at UC Berkeley is one I know, and he's very helpful.  (He and I
> both seem to have become itinerant globe-trotting microscopy educators!)
>
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
>
> by Guy Cox    CRC Press / Taylor & Francis
>
>     http://www.guycox.com/optical.htm
> <http://www.guycox.com/optical.htm>
>
> ______________________________________________
>
> Associate Professor Guy Cox, MA, DPhil(Oxon)
>
> Australian Centre for Microscopy & Microanalysis,
>
> Madsen Building F09, University of Sydney, NSW 2006
>
>
>
> Phone [phone]     Fax [phone]
>
>             Mobile [phone]
>
> ______________________________________________
>
>      http://www.guycox.net <http://www.guycox.net>
>
>
>
>
>
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Tuesday, 14 December 2010 12:59 PM
> To: [hidden email]
> Subject: Re: help with Zeiss NDD
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Without the scanhead and control box you are pretty much starting from
> scratch.  Unless you can get Zeiss to tell you how the NDDs work they
> won't
> be a whole lot of use to you.  You might be able to strip them right
> down to
> the PMTs and get the signals directly.  I'd recommend trying to get the
> missing pieces you need to turn it into a complete Zeiss (maybe somebody
> has
> traded in an LSM 510 you can get for cheap?) or scrap the NDDs and buy a
> 2
> photon conversion kit from thorlabs or somebody.  Since you need the
> scanhead, controller, and software you actually need quite a bit of
> hardware.
>
> Craig
>
>
>
>
> On Mon, Dec 13, 2010 at 6:40 PM, Robert Duvoisin <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi
> >
> > I have inherited parts of a 2-photons microscope. I have the 2 P laser
> > (Coherent) and a Zeiss Axioskop 2 FS mot. (I'm missing the LSM 510
> scanhead,
> > computer and control box). I need to build the scanhead and am hoping
> to run
> > it with ScanImage. Does anyone know how to control and acquire data
> from the
> > Zeiss NDDs, if possible including the motorized non-descanned
> detection kit
> > and the detection module PMT?
> >
> > I'm happy to provide more details if that helps. Any advice is also
> > welcome.
> > Thanks
> > Robert
> >
>
> ________________________________
>
> No virus found in this message.
> Checked by AVG - www.avg.com
> Version: 10.0.1170 / Virus Database: 426/3313 - Release Date: 12/13/10
>