high 700s nm detection with Zeiss 710?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We are thinking of using DiR for membrane labeling and imaging with the Zeiss 710 with three internal detectors with adjustable slits for wavelength. (We can use a pulsed laser at 750 nm for excitation.) Does anyone know where the internal detectors of the 710 top out. DiR emits with a peak at 788 nm and we want to know whether we may image it. The Zen software is ambiguous and the spec sheets on the Zeiss website don't say, or at least I haven't found the ones that do. Thank you. Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> |
 
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Michael Schell |
Re: high 700s nm detection with Zeiss 710?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I found a place on their spec sheet that claims that you are good out to 1100 nm. Have not tested this personally, YMMV. Michael >>> "Cammer, Michael" <[hidden email]> 02/04/11 3:45 PM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We are thinking of using DiR for membrane labeling and imaging with the Zeiss 710 with three internal detectors with adjustable slits for wavelength. (We can use a pulsed laser at 750 nm for excitation.) Does anyone know where the internal detectors of the 710 top out. DiR emits with a peak at 788 nm and we want to know whether we may image it. The Zen software is ambiguous and the spec sheets on the Zeiss website don't say, or at least I haven't found the ones that do. Thank you. Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> Classification: UNCLASSIFIED Caveats: None Classification: UNCLASSIFIED Caveats: None |
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Armstrong, Brian |
Re: high 700s nm detection with Zeiss 710?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, you should be able to find the specs, with curves, on the Hamamatsu web-site. On the LSM510 they were model #R6357 I believe. I do not know what model was used on the 710. Cheers, Brian D Armstrong PhD Assistant Research Professor Light Microscopy Core Beckman Research Institute City of Hope Dept of Neuroscience 1450 E Duarte Rd Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag ing/Pages/default.aspx -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michael Schell Sent: Friday, February 04, 2011 12:59 PM To: [hidden email] Subject: Re: high 700s nm detection with Zeiss 710? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I found a place on their spec sheet that claims that you are good out to 1100 nm. Have not tested this personally, YMMV. Michael >>> "Cammer, Michael" <[hidden email]> 02/04/11 3:45 PM >>> ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We are thinking of using DiR for membrane labeling and imaging with the Zeiss 710 with three internal detectors with adjustable slits for wavelength. (We can use a pulsed laser at 750 nm for excitation.) Does anyone know where the internal detectors of the 710 top out. DiR emits with a peak at 788 nm and we want to know whether we may image it. The Zen software is ambiguous and the spec sheets on the Zeiss website don't say, or at least I haven't found the ones that do. Thank you. Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> Classification: UNCLASSIFIED Caveats: None Classification: UNCLASSIFIED Caveats: None --------------------------------------------------------------------- SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. --------------------------------------------------------------------- |
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Mark Cannell |
Re: high 700s nm detection with Zeiss 710?
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In reply to this post by Michael Schell
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Could 1100 be an IR option? This says 750, and that is also selectable the software. Since we have normal PMTs the performance at 1100 would be poor -maybe even useless except for reflected light? You would also need IR corrected lenses. http://www.zeiss.com/C1256D18002CC306/0/BA710158046BB095C125749B004861E4/$file/60-1-0006.pdf "The basis is a filter-free spectral detection unit, which can be continuously set over the entire wavelength range from 390 to 750 nm" Hope this helps. Cheers Mark On 5/02/2011, at 9:59 AM, Michael Schell wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > I found a place on their spec sheet that claims that you are good > out to 1100 nm. Have not tested this personally, YMMV. > > Michael > >>>> "Cammer, Michael" <[hidden email]> 02/04/11 3:45 PM >>> > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We are thinking of using DiR for membrane labeling and imaging with > the Zeiss 710 with three internal detectors with adjustable slits > for wavelength. (We can use a pulsed laser at 750 nm for excitation.) > > Does anyone know where the internal detectors of the 710 top out. > DiR emits with a peak at 788 nm and we want to know whether we may > image it. The Zen software is ambiguous and the spec sheets on the > Zeiss website don't say, or at least I haven't found the ones that do. > > Thank you. > > Sincerely, > > Michael > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > > </PRE> > <html> > <body> > ------------------------------------------------------------<br /> > This email message, including any attachments, is for the sole use > of the intended recipient(s) and may contain information that is > proprietary, confidential, and exempt from disclosure under > applicable law. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you have received this email in error > please notify the sender by return email and delete the original > message. Please note, the recipient should check this email and any > attachments for the presence of viruses. The organization accepts no > liability for any damage caused by any virus transmitted by this > email.<br /> > ================================= > </body> > </html> > <PRE> > > Classification: UNCLASSIFIED > Caveats: None > > > Classification: UNCLASSIFIED > Caveats: None |
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Craig Brideau |
Re: high 700s nm detection with Zeiss 710?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Yeah, I'm pretty sure this type is used in the PMT-based multi-anode spectral detectors used by Nikon and Zeiss. I have several Nikon spectral units and they cover 400-750nm. Craig On Fri, Feb 4, 2011 at 2:36 PM, Mark Cannell <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Could 1100 be an IR option? > > This says 750, and that is also selectable the software. Since we have > normal PMTs the performance at 1100 would be poor -maybe even useless > except for reflected light? You would also need IR corrected lenses. > > > http://www.zeiss.com/C1256D18002CC306/0/BA710158046BB095C125749B004861E4/$file/60-1-0006.pdf > > "The basis is a filter-free spectral detection unit, > which can be continuously set over the > entire wavelength range from 390 to 750 > nm" > > Hope this helps. > > Cheers Mark > > > > On 5/02/2011, at 9:59 AM, Michael Schell wrote: > > ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I found a place on their spec sheet that claims that you are good out to >> 1100 nm. Have not tested this personally, YMMV. >> >> Michael >> >> "Cammer, Michael" <[hidden email]> 02/04/11 3:45 PM >>> >>>>> >>>> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> We are thinking of using DiR for membrane labeling and imaging with the >> Zeiss 710 with three internal detectors with adjustable slits for >> wavelength. (We can use a pulsed laser at 750 nm for excitation.) >> >> Does anyone know where the internal detectors of the 710 top out. DiR >> emits with a peak at 788 nm and we want to know whether we may image it. >> The Zen software is ambiguous and the spec sheets on the Zeiss website >> don't say, or at least I haven't found the ones that do. >> >> Thank you. >> >> Sincerely, >> >> Michael >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist >> Skirball Institute of Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> >> </PRE> >> <html> >> <body> >> ------------------------------------------------------------<br /> >> This email message, including any attachments, is for the sole use of the >> intended recipient(s) and may contain information that is proprietary, >> confidential, and exempt from disclosure under applicable law. Any >> unauthorized review, use, disclosure, or distribution is prohibited. If you >> have received this email in error please notify the sender by return email >> and delete the original message. Please note, the recipient should check >> this email and any attachments for the presence of viruses. The organization >> accepts no liability for any damage caused by any virus transmitted by this >> email.<br /> >> ================================= >> </body> >> </html> >> <PRE> >> >> Classification: UNCLASSIFIED >> Caveats: None >> >> >> Classification: UNCLASSIFIED >> Caveats: None >> > |
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Andreas Bruckbauer |
Re: high 700s nm detection with Zeiss 710?
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michael, you would also need to think about the microscope dichroics. When you use the pulsed laser which is usually used for 2 photon excitation, then the dichroic to couple this into the beam pass would most likely reflect everything above the given cutoff wavelength, when you use the 690 dichroic it will pass fluorescence below 690 nm but not above. A 80/20 splitter might do the trick but i am not sure if this is in there as standard. Another consideration is that the AOM will probably not allow you to adjust laser power low enough, but putting an additional filter in the beam pass should solve this easily. Some microscope manufactuers insert additional IR locking filter in front of their detector units, but i think for the LSM 710 this is not the case. best wishes Andreas -----Original Message----- From: Cammer, Michael <[hidden email]> To: [hidden email] Sent: Fri, 4 Feb 2011 20:44 Subject: high 700s nm detection with Zeiss 710? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We are thinking of using DiR for membrane labeling and imaging with the Zeiss 710 with three internal detectors with adjustable slits for wavelength. (We can use a pulsed laser at 750 nm for excitation.) Does anyone know where the internal detectors of the 710 top out. DiR emits with a peak at 788 nm and we want to know whether we may image it. The Zen software is ambiguous and the spec sheets on the Zeiss website don't say, or at least I haven't found the ones that do. Thank you. Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> |
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you all for suggestions. The result is that it did not work. Using the external detector, we can get a fuzzy widefield fluoresence image. Not useful for our current project and we have a widefield 'scope we could use for this anyhow. According to the software controls, the internal detectors collect up through 758 nm. However, testing with reflection mode with the longest wavelength physical gate, collection is practical only to 740 nm. After 740 nm collection intensity falls rapidly and is not detectable above 750 nm. Thanks again. Sincerely, Michael C. From: Cammer, Michael Sent: Friday, February 04, 2011 3:44 PM To: 'Confocal Microscopy List' Subject: high 700s nm detection with Zeiss 710? We are thinking of using DiR for membrane labeling and imaging with the Zeiss 710 with three internal detectors with adjustable slits for wavelength. (We can use a pulsed laser at 750 nm for excitation.) Does anyone know where the internal detectors of the 710 top out. DiR emits with a peak at 788 nm and we want to know whether we may image it. The Zen software is ambiguous and the spec sheets on the Zeiss website don't say, or at least I haven't found the ones that do. Thank you. Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> |
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