how to use volocity to measure total fluorescence intensity?

[Wu, Hao]

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Dear All,
I am wondering how to measure total fluorescence intensity using volocity. What I want to do is to circle out different region of a tissue or different area of a cell, and measure the total fluorescent intensity and compare these numbers. However, when I look at velocity tutorial, i could not find such a function.
Can someone please give some advice?
thank you so much
Sincerely
hao

[SUBSCRIBE CONFOCALMICROSCOPY Sara Smith]

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Hi Hao,

Volocity does have the feature to measure total intensity of the Region of
Interest (ROI). In the measurement tab, you may draw multiple ROIs. All
measurements such as intensity and area will be displayed in the
measurements table below the images.

Hope this helps.


Yi

On Thu, Jul 12, 2012 at 12:43 PM, Wu, Hao <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
> I am wondering how to measure total fluorescence intensity using volocity.
> What I want to do is to circle out different region of a tissue or
> different area of a cell, and measure the total fluorescent intensity and
> compare these numbers. However, when I look at velocity tutorial, i could
> not find such a function.
> Can someone please give some advice?
> thank you so much
> Sincerely
> hao

[Barlow, Andrew]

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Hello Hao,
PerkinElmer have a team of technical support specialists that can help you with this, or any other imaging issues that you have.

There are several ways to contact them, please follow the link below for details:

http://cellularimaging.perkinelmer.com/support/contact/

It's a good opportunity to mention that the team just don't just deal with conventional problems such a systems failures, but are very happy to advise with day to day imaging or analysis questions that you may have.

Regards,
Andrew


Andrew Barlow PhD | Applications Specialist
PerkinElmer | For the Better
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> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Wu, Hao
> Sent: 12 July 2012 17:44
> To: [hidden email]
> Subject: how to use volocity to measure total fluorescence intensity?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
> I am wondering how to measure total fluorescence intensity using
> volocity. What I want to do is to circle out different region of a
> tissue or different area of a cell, and measure the total fluorescent
> intensity and compare these numbers. However, when I look at velocity
> tutorial, i could not find such a function.
> Can someone please give some advice?
> thank you so much
> Sincerely
> hao