image databases
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Does anyone know of repositories of microscopy images
available to all researchers? There are people at our university interested and
capable of creating such databases, but we don’t know what already
exists. Thanks! Mike Model |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Check... http://openmicroscopy.org.uk/ Larry MODEL, MICHAEL wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Does anyone know of repositories of microscopy images available to all > researchers? There are people at our university interested and capable > of creating such databases, but we don’t know what already exists. Thanks! > > Mike Model > -- Larry Tague Co-Investigator BBHSL* Co-Director of MECCA** Research Associate (Dept. of Physiology) University of Tennessee Health Science Center 894 Union Ave. Memphis, TN 38163 Phone Bus.: 901-448-7152 Phone FAX: 901-448-7126 e-mail:[hidden email] or [hidden email] *BBHSL "Building Bridges to Health Science Literacy" URL: http://bbhsl.mecca.org, a Science Education Partnership Award (SEPA). Supported by the National Center of Research Resources (NCRR) of the National Institutes of Health (NIH). **MECCA (Memphis Educational Computer Connectivity Alliance) URL: http://www.mecca.org/. Originally support by the National Science Foundation's "Networking Infrastructure or Education" program. CONFIDENTIALITY NOTICE: This email and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. This communication may contain protected health information, or other legally privileged, confidential, or proprietary information. If you are not the intended recipient or the individual responsible for delivering the email to the recipient, please be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error, please immediately notify the sender, disregard the foregoing message, and delete the message. We apologize for any inconvenience this may have caused. IMPORTANT NOTE: Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations. |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Many great ones out there. Three I use, From American Society for Cell Biology http://cellbase.ascb.org/classic.html From Michael Davidson at Florida State Univ/Molecular Expression web site http://www.molecularexpressions.com/micro/gallery.html And finally from Molecular Probes/Invitrogen. http://probes.invitrogen.com/servlets/gallery/ Mike Ignatius Molecular Probes/Invitrogen. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Larry Tague Sent: Wednesday, February 06, 2008 1:40 PM To: [hidden email] Subject: Re: image databases Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Check... http://openmicroscopy.org.uk/ Larry MODEL, MICHAEL wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Does anyone know of repositories of microscopy images available to all > researchers? There are people at our university interested and capable > of creating such databases, but we don't know what already exists. Thanks! > > Mike Model > -- Larry Tague Co-Investigator BBHSL* Co-Director of MECCA** Research Associate (Dept. of Physiology) University of Tennessee Health Science Center 894 Union Ave. Memphis, TN 38163 Phone Bus.: 901-448-7152 Phone FAX: 901-448-7126 e-mail:[hidden email] or [hidden email] *BBHSL "Building Bridges to Health Science Literacy" URL: http://bbhsl.mecca.org, a Science Education Partnership Award (SEPA). Supported by the National Center of Research Resources (NCRR) of the National Institutes of Health (NIH). **MECCA (Memphis Educational Computer Connectivity Alliance) URL: http://www.mecca.org/. Originally support by the National Science Foundation's "Networking Infrastructure or Education" program. CONFIDENTIALITY NOTICE: This email and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. This communication may contain protected health information, or other legally privileged, confidential, or proprietary information. If you are not the intended recipient or the individual responsible for delivering the email to the recipient, please be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error, please immediately notify the sender, disregard the foregoing message, and delete the message. We apologize for any inconvenience this may have caused. IMPORTANT NOTE: Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations. |
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In reply to this post by mmodel
Search the CONFOCAL archive at
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Hi Mike,
Here are some resources (no commercial interest in any): http://www.biologyimagelibrary.com/ http://www.microscopyu.com/galleries/index.html http://www.olympusfluoview.com/gallery/index.html http://www.microscopy-uk.net/coppermine/index.php?cat=1 http://www.mrs.org/s_mrs/doc.asp?CID=1803&DID=171434 Graham ----- Original Message ----- From: "MICHAEL MODEL" <[hidden email]> To: [hidden email] Sent: Thursday, February 7, 2008 5:07:28 AM (GMT+0800) Asia/Hong_Kong Subject: image databases Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Does anyone know of repositories of microscopy images available to all researchers? There are people at our university interested and capable of creating such databases, but we don’t know what already exists. Thanks!
Mike Model
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In reply to this post by mmodel
Search the CONFOCAL archive at
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Mike please look at http://biodidac.bio.uottawa.ca/
They also list other sites with images Rick, From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Does
anyone know of repositories of microscopy images available to all researchers?
There are people at our university interested and capable of creating such
databases, but we don’t know what already exists. Thanks! Mike
Model |
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In reply to this post by Graham Wright-5
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear List, I am considering placing a Faraday rotator or isolator in front of a two-photon laser and am wondering what others have experienced with such a configuration. I am currently experiencing a lot of "feedback", ie return reflected laser, that is wrecking havoc with my laser's stability. When it senses the reflected light, it can not maintain a mode locked state and can only lase in CW. I'm heard that a Faraday rotator should be sufficient, but I am wondering if an isolator is necessary to stop the reflected light from entering the cavity of the laser source. Also, I would love to get a recommendation for a reliable company that makes them. Many thanks for any advice, Ann -- Ann Haberman, PhD Department of Laboratory Medicine Yale University School of Medicine 1 Gilbert St. TAC S541 New Haven, CT 06510 203-785-7349 203-785-5415 (fax) [hidden email] |
 
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Hyungsik Lim |
Re: Faraday rotators and isolators
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Ann, An isolator is simply a Faraday rotator sandwiched with two polarizers. Without those polarizers, your feedback into the laser cavity (i.e. back-reflection) will be orthogonally polarized to the output beam. It is likely that the laser will be little disturbed in this case, because the TiSap laser cavity favors one polarization. However complete (>30dB) suppression of feedback will be achieved only with an isolator. I would recommend the following companies for an isolator. Please also keep in mind the group-velocity dispersion, if you are using <30fs pulses. http://www.isowave.com/ http://www.ofr.com/ Best, Hyungsik -- Hyungsik Lim, Ph.D. Postdoctoral Associate, Webb Group Applied Physics, Cornell Univ. 607-255-8034 |
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Craig Brideau |
Re: Faraday rotators and isolators
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In reply to this post by Graham Wright-5
Search the CONFOCAL archive at
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A Faraday rotator isolator is a large chunk of crystal. It will cause a lot of dispersion to your pulses if you are using a femtosecond laser. Our own laser went from 70fs to 300fs after passing through ours. This effect can be minimized by getting as short a crystal as possible in your isolator.
EOT http://www.eotech.com/ has decent isolators although it can be hard to tell which one has the shortest crystal. We originally selected ours based on the assumption that the smallest entrance aperture will have the shortest crystal. This turned out to not be the case, so check with the company before buying. Last I heard recently that their 8mm aperture unit: http://www.eotech.com/store/products.php?categoryParentName=Faraday+Rotators+%26+Isolators&categoryName=Broadband++%28Ti%3ASapphire%29+Rotators+%26+Isolators actually had the shortest crystal, but make sure to check with the company first. Regarding the utility of an isolator, if you have any reflections at all having one is critical for the stability of your laser. In some laser designs the reflected light can actually be physically harmful to the laser as well! Craig On Thu, Feb 14, 2008 at 11:51 AM, Ann Haberman <[hidden email]> wrote: Search the CONFOCAL archive at |
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In reply to this post by Haberman, Ann
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello: I used to have a tripled Yag pumped dye laser. I forget if they put a 1/4 waveplate or a 1/2 waveplate to prevent reflections from affecting the laser between the dye laser and the Yag. The reflections do enter the laser but the polarization rotation prevents interaction. If you put a 1/2 waveplate, that will rotate the polarization of the laser beam entering you microscpe, so you will have to account for that. Ensure that the waveplate can handle the laser power involved. Also, my Mira can loose modelock when I use my harmonic generator with a 'dull surface' crystal. The reflections from the crystals are the problem. The new crystals do not do this. Input windows with a wedge or off-axis windows are sometimes used to redirect reflections. You might want to see what causes the reflection(s) first. Good luck! Sophie ____________________________________________________ Sophie M. K. Brunet, Ph. D. Research Officer Optical Spectroscopy, Laser Systems and Applications Chemistry 112 sessional lecturer [hidden email] 306-966-1719 (office) 306-966-1702 (fax) ____________________________________________________ Saskatchewan Structural Sciences Centre University of Saskatchewan Thorvaldson Bldg. 110 Science Place Saskatoon, Sk S7N 5C9 ____________________________________________________ Quoting Ann Haberman <[hidden email]>: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Dear List, > > I am considering placing a Faraday rotator or isolator in front of a > two-photon laser and am wondering what others have experienced with > such a configuration. > > I am currently experiencing a lot of "feedback", ie return reflected > laser, that is wrecking havoc with my laser's stability. When it > senses the reflected light, it can not maintain a mode locked state > and can only lase in CW. > > I'm heard that a Faraday rotator should be sufficient, but I am > wondering if an isolator is necessary to stop the reflected light > from entering the cavity of the laser source. Also, I would love to > get a recommendation for a reliable company that makes them. > > Many thanks for any advice, > Ann > -- > > Ann Haberman, PhD > Department of Laboratory Medicine > Yale University School of Medicine > 1 Gilbert St. > TAC S541 > New Haven, CT 06510 > > 203-785-7349 > 203-785-5415 (fax) > [hidden email] > |
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Haberman, Ann |
Re: Faraday rotators and isolators
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In reply to this post by Craig Brideau
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear List,
Thank you all for the many helpful suggestions.
One of the manufacturers of a Faraday isolator feels that a
rotator by itself would be insufficient to reduce the reflected laser
and indicated that a isolator would be necessary. Given the amount of
dispersion I would get with just a rotator, I'm loathe to get an
isolator. Their assumption is that the polarization of the reflected
laser will be identical to the laser output. Is likely that it would
be different ?
The suggestion of using a 1/2 or 1/4 waveplate sounds like a good
idea. Would there be less dispersion in this arrangement? What would
be disadvantages of this compared to the rotator?
Many thanks again for this helpful advice,
Ann
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal A Faraday rotator isolator is a large chunk of crystal. It will cause a lot of dispersion to your pulses if you are using a femtosecond laser. Our own laser went from 70fs to 300fs after passing through ours. This effect can be minimized by getting as short a crystal as possible in your isolator. On Thu, Feb 14, 2008 at 11:51 AM, Ann Haberman <[hidden email]> wrote: -- Ann Haberman, PhD Department of Laboratory Medicine Yale University School of Medicine 1 Gilbert St. TAC S541 New Haven, CT 06510 203-785-7349 203-785-5415 (fax) [hidden email] |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello: If you choose a waveplate or a rotator, ask the manufacturer about wavelength range and power threshold. In either case, the travel path through the optic is a lot less than a Faraday Isolator. The double Fresnel-Rhomb is ONE KIND of rotator with 'broad' wavelength range, but the travel through the optical material is big. Make sure you don't have a front reflection from the optic (I do not know if they can AR coat these optics). I any case, if your laser is tunable... this may be a bigger obstacle. You do not want to realign for each wavelength. I would chase down the main source of reflection first. Anyone else had to solve this problem? My beam path is so big, it is not a problem. CVI tends to give good advice on their optics and knows about short pulsed lasers. No financial interest. I do not know if the merge/purchase of Melles Griot changes things. Plenty of suppliers for these types of parts though and any of them should be helpful. Good luck! Sophie ____________________________________________________ Sophie M. K. Brunet, Ph. D. Research Officer Optical Spectroscopy, Laser Systems and Applications Chemistry 112 sessional lecturer [hidden email] 306-966-1719 (office) 306-966-1702 (fax) ____________________________________________________ Saskatchewan Structural Sciences Centre University of Saskatchewan Thorvaldson Bldg. 110 Science Place Saskatoon, Sk S7N 5C9 ____________________________________________________ Quoting Ann Haberman <[hidden email]>: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Dear List, > > Thank you all for the many helpful suggestions. > > One of the manufacturers of a Faraday isolator feels that a rotator > by itself would be insufficient to reduce the reflected laser and > indicated that a isolator would be necessary. Given the amount of > dispersion I would get with just a rotator, I'm loathe to get an > isolator. Their assumption is that the polarization of the reflected > laser will be identical to the laser output. Is likely that it would > be different ? > > The suggestion of using a 1/2 or 1/4 waveplate sounds like a good > idea. Would there be less dispersion in this arrangement? What would > be disadvantages of this compared to the rotator? > > Many thanks again for this helpful advice, > Ann > > -- > > Ann Haberman, PhD > Department of Laboratory Medicine > Yale University School of Medicine > 1 Gilbert St. > TAC S541 > New Haven, CT 06510 > > 203-785-7349 > 203-785-5415 (fax) > [hidden email] > |
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Craig Brideau |
Re: Faraday rotators and isolators
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In reply to this post by Craig Brideau
Search the CONFOCAL archive at
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The Faraday isolator gives the best isolation, but if you use a 1/4 waveplate and a polarizer you can also make a basic isolator. The drawback is: If your laser is tuneable you will need a BROADBAND 1/4 waveplate, which costs about $800 -starting to approach the cost of the Faraday isolator. The 1/4 waveplate method also means that your laser (post isolator) will have circular polarization. If you are doing any non-linear work with your microscope this can reduce the efficiency of some interactions. The Faraday isolator allows you to retain linear polarization, and tends to have better overall isolation. Again, if you pick one with a short crystal path you can keep the dispersion down to a minimum.
Also, the dispersion you would expect to see totally depends on the bandwidth of your laser. If your laser is 130fs or greater, with transform limited bandwidth, your dispersion will be minimal, even through a thick isolator. If your pulse width is below 130fs, then you will need to find the shortest-path isolator you can. Craig Brideau On Feb 15, 2008 10:53 AM, Ann Haberman <[hidden email]> wrote:
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Sudipta Maiti |
Re: Faraday rotators and isolators
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In reply to this post by Haberman, Ann
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal The dispersion on a half wave plate- polarizer combination should be lower. But do remember that they are very wavelength specific. You probably need one for every 25 nm. Sudipta On Fri, 15 Feb 2008, Ann Haberman wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Dear List, > > Thank you all for the many helpful suggestions. > > One of the manufacturers of a Faraday isolator feels that a rotator by itself > would be insufficient to reduce the reflected laser and indicated that a > isolator would be necessary. Given the amount of dispersion I would get with > just a rotator, I'm loathe to get an isolator. Their assumption is that the > polarization of the reflected laser will be identical to the laser output. Is > likely that it would be different ? > > The suggestion of using a 1/2 or 1/4 waveplate sounds like a good idea. Would > there be less dispersion in this arrangement? What would be disadvantages of > this compared to the rotator? > > Many thanks again for this helpful advice, > Ann > > >> Search the CONFOCAL archive at >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal A Faraday rotator >> isolator is a large chunk of crystal. It will cause a lot of dispersion to >> your pulses if you are using a femtosecond laser. Our own laser went from >> 70fs to 300fs after passing through ours. This effect can be minimized by >> getting as short a crystal as possible in your isolator. >> EOT <http://www.eotech.com/>http://www.eotech.com/ has decent isolators >> although it can be hard to tell which one has the shortest crystal. We >> originally selected ours based on the assumption that the smallest entrance >> aperture will have the shortest crystal. This turned out to not be the >> case, so check with the company before buying. Last I heard recently that >> their 8mm aperture unit: >> <http://www.eotech.com/store/products.php?categoryParentName=Faraday+Rotators+%26+Isolators&categoryName=Broadband++%28Ti%3ASapphire%29+Rotators+%26+Isolators>http://www.eotech.com/store/products.php?categoryParentName=Faraday+Rotators+%26+Isolators&categoryName=Broadband++%28Ti%3ASapphire%29+Rotators+%26+Isolators >> actually had the shortest crystal, but make sure to check with the company >> first. >> Regarding the utility of an isolator, if you have any reflections at all >> having one is critical for the stability of your laser. In some laser >> designs the reflected light can actually be physically harmful to the laser >> as well! >> >> Craig >> >> >> On Thu, Feb 14, 2008 at 11:51 AM, Ann Haberman >> <<mailto:[hidden email]>[hidden email]> wrote: >> >> Search the CONFOCAL archive at >> <http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >> >> Dear List, >> >> I am considering placing a Faraday rotator or isolator in front of a >> two-photon laser and am wondering what others have experienced with >> such a configuration. >> >> I am currently experiencing a lot of "feedback", ie return reflected >> laser, that is wrecking havoc with my laser's stability. When it >> senses the reflected light, it can not maintain a mode locked state >> and can only lase in CW. >> >> I'm heard that a Faraday rotator should be sufficient, but I am >> wondering if an isolator is necessary to stop the reflected light >> from entering the cavity of the laser source. Also, I would love to >> get a recommendation for a reliable company that makes them. >> >> Many thanks for any advice, >> Ann >> -- >> >> Ann Haberman, PhD >> Department of Laboratory Medicine >> Yale University School of Medicine >> 1 Gilbert St. >> TAC S541 >> New Haven, CT 06510 >> >> 203-785-7349 >> 203-785-5415 (fax) >> <mailto:[hidden email]>[hidden email] > > > -- Sudipta Maiti Dept. of Chemical Sciences Tata Institute of Fundamental Research Homi Bhabha Road, Colaba, Mumbai 400005, India 91-22-2278-2716 www.tifr.res.in/~biophotonics |
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