|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dear colleagues,
I need your advice on staining hippocampal mouse neurons. I am transducing these neurons with a GFP expressing lenti virus shRNA library and after a few days I observe a "beading/swelling" in the GFP channel in the neurites.
I would like to stain exclusively neurons in this neuron/glia mixed culture. I have the possibility to apply a dye while the neurons are still alive or stain them after fixation. The dye should stain the whole cytosol of the neuron, so I can determine the health status of the neuron, membrane integrity, etc.
I read so far that a lot of people use NSE (neuron specific enolase) antibodies to stain for neurons. Does anybody know if they are staining the entire cell homogeneously?
I also thought to apply Calcein to stain the cells, but then I would run in the problem that i would also stain the glia cells. Or does anybody know a calcein equivalent that exclusively stains neurons?
I'll be very happy for any ideas you might have.
Thanks a lot,
Gunnar Poplawski
|
Locked
