imaging red blood cells in multiphoton

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Richard Cole Richard Cole
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imaging red blood cells in multiphoton

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

As anyone imaged RBC via there autofluorescence with multi-photon confocal?

Thanks in advance.

Richard Cole
Research Scientist IV
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
P.O. Box 509 Albany N.Y. 12201-0509
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Eric Scarfone Eric Scarfone
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Re: imaging red blood cells in multiphoton

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

you may want to check Warren Zipfel et al. (Science, may 2003). They
imaged rbc indirectly by filling capillaries with Q-dots.
Cheers
Eric

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
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Karolinska Institutet

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----- Original Message -----
From: Richard Cole <[hidden email]>
Date: Thursday, September 18, 2008 1:41 am
Subject: imaging red blood cells in multiphoton
To: [hidden email]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> As anyone imaged RBC via there autofluorescence with multi-photon
> confocal?
> Thanks in advance.
>
> Richard Cole
> Research Scientist IV
> Director: Advanced Light Microscopy & Image Analysis Core
> Wadsworth Center
> P.O. Box 509 Albany N.Y. 12201-0509
> * 518-474-7048
> ** 518-474-4430  
> * [hidden email]
> Website www.wadsworth.org/cores/alm/index.htm
>
Jerry Sedgewick-2 Jerry Sedgewick-2
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Re: imaging red blood cells in multiphoton

In reply to this post by Richard Cole
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I recall seeing RBCs in zebrafish embryos at 820nm, but I also recall
that the RBCs were visible via autofluorescence along a range of
excitation values from 820nm to 870nm or so.

Jerry

Richard Cole wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> As anyone imaged RBC via there autofluorescence with multi-photon confocal?
>
> Thanks in advance.
>
> Richard Cole
> Research Scientist IV
> Director: Advanced Light Microscopy & Image Analysis Core
> Wadsworth Center
> P.O. Box 509 Albany N.Y. 12201-0509
> * 518-474-7048
> ** 518-474-4430  
> * [hidden email]
> Website www.wadsworth.org/cores/alm/index.htm
> .
>
>  


--
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Program Director, Biomedical Image Processing Lab (BIPL)
Department of Neuroscience, University of Minnesota
312 Church St. SE, 1-205 Hasselmo Hall
Minneapolis, MN  55455
(612) 624-6607
[hidden email]
http://www.bipl.umn.edu
Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output."

Rawlight.com (dba Sedgewick Initiatives)
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