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I want to put together the most cost effective confocal-like system to do single plane time lapse imaging with relatively light sensitive cells (Dictyostelium). I have a very limited budget, and this is for teaching, not research so maximum resolution is not an issue. Here is the list of approaches that seem possible. Any comments appreciated:
Grid confocal: how many sections does it take and how long does it take to acquire? Can you do live cells that are moving? Deconvolution: If you only want one focal plane, what can you get away with in terms of minimum focal planes of capture to help with deblurring Olympus DSU: ? Any others I should be looking into? Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
Tim Fletcher-4 |
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi David,
we resell the CARV II widefield confocal from
BD (but not in your area) - Its easy to use, fast (50FPS is
achievable), not as cheap as a grid confocal, but certainly worth seeing if
your budget can stretch to it.
More details can be found here - http://www.bdbiosciences.com/pharmingen/products/display_product.php?keyID=99
Feel free to contact me directly if you have
difficulty getting information or wish to see some example images
etc.
Regards,
Tim.
Tim Fletcher
Sales Manager IMAGE SOLUTIONS (UK) Limited Expertise in Scientific Imaging Imsol House 2 Cable Court Pittman Way Fulwood Preston PR2 9YW United Kingdom. Mob +44(0) 7968 428836 Tel. +44 (0) 1772 663140 Fax. +44 (0) 1772 663150
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