insect imaging

[leoncio vergara]

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Does anybody know of a technique to image through the cuticle of mosquitoes? We were wondering if it is possible to do multiphoton imaging in whole mosquitoes (dead or alive). Any help would be greatly appreciated. Or alternative techniques like some way of partially removing the cuticle. We are intrested on the organs that participate in viral cycles (midgut, malpigian tubes, ovaries, salivary glands).

Leoncio

[Nuno Moreno]

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Leoncio,

For that particular case I would suggest DSLM/SPIM. Bear in mind that if it is too deep you wont be able to do it with conventional optical microscopy, SPIM, TPE, etc.

Best,
Nuno Moreno, PhD
Instituto Gulbenkian de Ciência




On Jan 15, 2013, at 1:54 PM, Vergara, Leoncio A. wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Does anybody know of a technique to image through the cuticle of mosquitoes? We were wondering if it is possible to do multiphoton imaging in whole mosquitoes (dead or alive). Any help would be greatly appreciated. Or alternative techniques like some way of partially removing the cuticle. We are intrested on the organs that participate in viral cycles (midgut, malpigian tubes, ovaries, salivary glands).
>
> Leoncio

[Tim Feinstein-2]

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Perhaps this new "miracle" reagent called ScaleA2 (http://www.riken.jp/engn/r-world/info/release/press/2011/110830_3/index.html) could help a little with imaging through the cuticle. Never tried it and can't make promises, but it may be worth a try.  

cheers,


TF

Timothy Feinstein, PhD
Visiting Research Associate
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA  15261

On Jan 15, 2013, at 8:54 AM, Vergara, Leoncio A. wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Does anybody know of a technique to image through the cuticle of mosquitoes? We were wondering if it is possible to do multiphoton imaging in whole mosquitoes (dead or alive). Any help would be greatly appreciated. Or alternative techniques like some way of partially removing the cuticle. We are intrested on the organs that participate in viral cycles (midgut, malpigian tubes, ovaries, salivary glands).
>
> Leoncio

[Craig Brideau]

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I've bugged Zeiss a bit to demo their 'Scale Optimized' lens, but they
don't have many to loan out, surprisingly. @:-)

Craig


On Tue, Jan 15, 2013 at 8:45 AM, Tim Feinstein <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Perhaps this new "miracle" reagent called ScaleA2 (
> http://www.riken.jp/engn/r-world/info/release/press/2011/110830_3/index.html)
> could help a little with imaging through the cuticle. Never tried it and
> can't make promises, but it may be worth a try.
>
> cheers,
>
>
> TF
>
> Timothy Feinstein, PhD
> Visiting Research Associate
> Laboratory for GPCR Biology
> Dept. of Pharmacology & Chemical Biology
> University of Pittsburgh, School of Medicine
> BST W1301, 200 Lothrop St.
> Pittsburgh, PA  15261
>
> On Jan 15, 2013, at 8:54 AM, Vergara, Leoncio A. wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Does anybody know of a technique to image through the cuticle of
> mosquitoes? We were wondering if it is possible to do multiphoton imaging
> in whole mosquitoes (dead or alive). Any help would be greatly appreciated.
> Or alternative techniques like some way of partially removing the cuticle.
> We are intrested on the organs that participate in viral cycles (midgut,
> malpigian tubes, ovaries, salivary glands).
> >
> > Leoncio
>

[Graham Wright-2]

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Leoncio,

Similar to Timothy's suggestion, here is a paper on optical clearing in
insects for 3D imaging (Drosophila melanogaster in this case) from McGurk
et al.:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0000834

Regards,
Graham

--
Dr Graham Wright
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

O:   [phone]
E:   [hidden email]
W:  http://www.imb.a-star.edu.sg/imu/


On 16 January 2013 02:20, Craig Brideau <[hidden email]> wrote:

>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I've bugged Zeiss a bit to demo their 'Scale Optimized' lens, but they
> don't have many to loan out, surprisingly. @:-)
>
> Craig
>
>
> On Tue, Jan 15, 2013 at 8:45 AM, Tim Feinstein <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Perhaps this new "miracle" reagent called ScaleA2 (
> >

http://www.riken.jp/engn/r-world/info/release/press/2011/110830_3/index.html
)

> > could help a little with imaging through the cuticle. Never tried it and
> > can't make promises, but it may be worth a try.
> >
> > cheers,
> >
> >
> > TF
> >
> > Timothy Feinstein, PhD
> > Visiting Research Associate
> > Laboratory for GPCR Biology
> > Dept. of Pharmacology & Chemical Biology
> > University of Pittsburgh, School of Medicine
> > BST W1301, 200 Lothrop St.
> > Pittsburgh, PA  15261
> >
> > On Jan 15, 2013, at 8:54 AM, Vergara, Leoncio A. wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Does anybody know of a technique to image through the cuticle of
> > mosquitoes? We were wondering if it is possible to do multiphoton

imaging
> > in whole mosquitoes (dead or alive). Any help would be greatly
appreciated.
> > Or alternative techniques like some way of partially removing the
cuticle.
> > We are intrested on the organs that participate in viral cycles (midgut,
> > malpigian tubes, ovaries, salivary glands).
> > >
> > > Leoncio
> >