inverting photo detector polarity on a microscope

[Michael Giacomelli]

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Hi,

I would like to test out a new detector module in a commerical Olympus
confocal body.  Unfortunately, the detector uses an inverting amplifier
(0->-1 V), while the Olympus assumes a non-inverting transimpedance
amplifier (0->+1 V).  I've spoken with them and there is no way to change
the A/D configuration on this specific model, and so I cannot use the
detector as is.

Is there a commerical solution available for adapting something like this?
I would like to avoid having to design and breadboard an inverting buffer
amplifier if I can avoid it.

Thanks,
Mike

[Craig Brideau]

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These Op Amp evaluation boards should get you there. Just make sure to get
a rail-to-rail version and buy the appropriate +/-5V (or whatever) supply
to handle the down swing.
https://www.digikey.com/products/en/development-boards-kits-programmers/evaluation-boards-op-amps/788?k=instrumentation+amp&k=&pkeyword=instrumentation+amp&FV=ffe00314&quantity=0&ColumnSort=0&page=1&stock=1&pageSize=25

Craig

On Wed, Sep 5, 2018 at 12:15 PM Michael Giacomelli <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I would like to test out a new detector module in a commerical Olympus
> confocal body.  Unfortunately, the detector uses an inverting amplifier
> (0->-1 V), while the Olympus assumes a non-inverting transimpedance
> amplifier (0->+1 V).  I've spoken with them and there is no way to change
> the A/D configuration on this specific model, and so I cannot use the
> detector as is.
>
> Is there a commerical solution available for adapting something like this?
> I would like to avoid having to design and breadboard an inverting buffer
> amplifier if I can avoid it.
>
> Thanks,
> Mike
>

[Edward Allgeyer]

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Hi Mike,

I have no commercial interest in either of the things I'm going to mention
but they maybe of interest to you.

First, there is a California based company, Pulse Research Lab, that sells
a selection of high speed off the shelf comparators/sine wave converters
that I've had success with for similar purposes. Their comparators are
fairly forgiving with what you connect as the input and you have a number
of options for the corresponding output. You can browse their selection
here:

https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1

I'll also mention that on their comparators they have preset thresholds but
you can also set an arbitrary threshold by applying a DC voltage to the
complementary input if you want to.

Second, Picoquant also sells a few inverters and adaptors that might work
for you. Specifically the SI100 is a passive inline pulse inverter and
might work for your application. You can browse their adapters, splitters
and cables here:

https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups

Hopefully this helps. Again, no commercial interest in either of these
companies. Just thought I'd mention them as possible options.

Best,
Ed

On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I would like to test out a new detector module in a commerical Olympus
> confocal body.  Unfortunately, the detector uses an inverting amplifier
> (0->-1 V), while the Olympus assumes a non-inverting transimpedance
> amplifier (0->+1 V).  I've spoken with them and there is no way to change
> the A/D configuration on this specific model, and so I cannot use the
> detector as is.
>
> Is there a commerical solution available for adapting something like this?
> I would like to avoid having to design and breadboard an inverting buffer
> amplifier if I can avoid it.
>
> Thanks,
> Mike
>

[Mark Cannell-2]

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You could just record the data as is and invert the image afterwards?

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD
 
[hidden email]
 
 

On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward Allgeyer" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Hi Mike,
   
    I have no commercial interest in either of the things I'm going to mention
    but they maybe of interest to you.
   
    First, there is a California based company, Pulse Research Lab, that sells
    a selection of high speed off the shelf comparators/sine wave converters
    that I've had success with for similar purposes. Their comparators are
    fairly forgiving with what you connect as the input and you have a number
    of options for the corresponding output. You can browse their selection
    here:
   
    https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
   
    I'll also mention that on their comparators they have preset thresholds but
    you can also set an arbitrary threshold by applying a DC voltage to the
    complementary input if you want to.
   
    Second, Picoquant also sells a few inverters and adaptors that might work
    for you. Specifically the SI100 is a passive inline pulse inverter and
    might work for your application. You can browse their adapters, splitters
    and cables here:
   
    https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
   
    Hopefully this helps. Again, no commercial interest in either of these
    companies. Just thought I'd mention them as possible options.
   
    Best,
    Ed
   
    On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]> wrote:
   
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    > Post images on http://www.imgur.com and include the link in your posting.
    > *****
    >
    > Hi,
    >
    > I would like to test out a new detector module in a commerical Olympus
    > confocal body.  Unfortunately, the detector uses an inverting amplifier
    > (0->-1 V), while the Olympus assumes a non-inverting transimpedance
    > amplifier (0->+1 V).  I've spoken with them and there is no way to change
    > the A/D configuration on this specific model, and so I cannot use the
    > detector as is.
    >
    > Is there a commerical solution available for adapting something like this?
    > I would like to avoid having to design and breadboard an inverting buffer
    > amplifier if I can avoid it.
    >
    > Thanks,
    > Mike
    >
   

[Craig Brideau]

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*****

You'd still need to apply a 2V offset then come down from that. By the time
you splice in the bias offset you might as well have patched in an inverter.
Craig

On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> You could just record the data as is and invert the image afterwards?
>
> Mark B. Cannell. Ph.D. FRSNZ FISHR
> Department of Physiology, Pharmacology & Neuroscience
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
>
> [hidden email]
>
>
>
> On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
> Allgeyer" <[hidden email] on behalf of [hidden email]>
> wrote:
>
>     *****
>     To join, leave or search the confocal microscopy listserv, go to:
>     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     Post images on http://www.imgur.com and include the link in your
> posting.
>     *****
>
>     Hi Mike,
>
>     I have no commercial interest in either of the things I'm going to
> mention
>     but they maybe of interest to you.
>
>     First, there is a California based company, Pulse Research Lab, that
> sells
>     a selection of high speed off the shelf comparators/sine wave
> converters
>     that I've had success with for similar purposes. Their comparators are
>     fairly forgiving with what you connect as the input and you have a
> number
>     of options for the corresponding output. You can browse their selection
>     here:
>
>
> https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
>
>     I'll also mention that on their comparators they have preset
> thresholds but
>     you can also set an arbitrary threshold by applying a DC voltage to the
>     complementary input if you want to.
>
>     Second, Picoquant also sells a few inverters and adaptors that might
> work
>     for you. Specifically the SI100 is a passive inline pulse inverter and
>     might work for your application. You can browse their adapters,
> splitters
>     and cables here:
>
>
> https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
>
>     Hopefully this helps. Again, no commercial interest in either of these
>     companies. Just thought I'd mention them as possible options.
>
>     Best,
>     Ed
>
>     On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]>
> wrote:
>
>     > *****
>     > To join, leave or search the confocal microscopy listserv, go to:
>     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     > Post images on http://www.imgur.com and include the link in your
> posting.
>     > *****
>     >
>     > Hi,
>     >
>     > I would like to test out a new detector module in a commerical
> Olympus
>     > confocal body.  Unfortunately, the detector uses an inverting
> amplifier
>     > (0->-1 V), while the Olympus assumes a non-inverting transimpedance
>     > amplifier (0->+1 V).  I've spoken with them and there is no way to
> change
>     > the A/D configuration on this specific model, and so I cannot use the
>     > detector as is.
>     >
>     > Is there a commerical solution available for adapting something like
> this?
>     > I would like to avoid having to design and breadboard an inverting
> buffer
>     > amplifier if I can avoid it.
>     >
>     > Thanks,
>     > Mike
>     >
>
>
>

[Michael Giacomelli]

*****
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*****

Thanks Craig.  It looks like I could modify the  THS3120EVM to operate at a
gain of -1 by swapping out some resistors.  The opamp itself is unity gain
stable.

Mike

On Thu, Sep 6, 2018 at 12:43 PM Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> You'd still need to apply a 2V offset then come down from that. By the time
> you splice in the bias offset you might as well have patched in an
> inverter.
> Craig
>
> On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > You could just record the data as is and invert the image afterwards?
> >
> > Mark B. Cannell. Ph.D. FRSNZ FISHR
> > Department of Physiology, Pharmacology & Neuroscience
> > School of Medical Sciences
> > University Walk
> > Bristol BS8 1TD
> >
> > [hidden email]
> >
> >
> >
> > On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
> > Allgeyer" <[hidden email] on behalf of [hidden email]
> >
> > wrote:
> >
> >     *****
> >     To join, leave or search the confocal microscopy listserv, go to:
> >     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >     Post images on http://www.imgur.com and include the link in your
> > posting.
> >     *****
> >
> >     Hi Mike,
> >
> >     I have no commercial interest in either of the things I'm going to
> > mention
> >     but they maybe of interest to you.
> >
> >     First, there is a California based company, Pulse Research Lab, that
> > sells
> >     a selection of high speed off the shelf comparators/sine wave
> > converters
> >     that I've had success with for similar purposes. Their comparators
> are
> >     fairly forgiving with what you connect as the input and you have a
> > number
> >     of options for the corresponding output. You can browse their
> selection
> >     here:
> >
> >
> >
> https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
> >
> >     I'll also mention that on their comparators they have preset
> > thresholds but
> >     you can also set an arbitrary threshold by applying a DC voltage to
> the
> >     complementary input if you want to.
> >
> >     Second, Picoquant also sells a few inverters and adaptors that might
> > work
> >     for you. Specifically the SI100 is a passive inline pulse inverter
> and
> >     might work for your application. You can browse their adapters,
> > splitters
> >     and cables here:
> >
> >
> >
> https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
> >
> >     Hopefully this helps. Again, no commercial interest in either of
> these
> >     companies. Just thought I'd mention them as possible options.
> >
> >     Best,
> >     Ed
> >
> >     On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]>
> > wrote:
> >
> >     > *****
> >     > To join, leave or search the confocal microscopy listserv, go to:
> >     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >     > Post images on http://www.imgur.com and include the link in your
> > posting.
> >     > *****
> >     >
> >     > Hi,
> >     >
> >     > I would like to test out a new detector module in a commerical
> > Olympus
> >     > confocal body.  Unfortunately, the detector uses an inverting
> > amplifier
> >     > (0->-1 V), while the Olympus assumes a non-inverting transimpedance
> >     > amplifier (0->+1 V).  I've spoken with them and there is no way to
> > change
> >     > the A/D configuration on this specific model, and so I cannot use
> the
> >     > detector as is.
> >     >
> >     > Is there a commerical solution available for adapting something
> like
> > this?
> >     > I would like to avoid having to design and breadboard an inverting
> > buffer
> >     > amplifier if I can avoid it.
> >     >
> >     > Thanks,
> >     > Mike
> >     >
> >
> >
> >
>

[Zdenek Svindrych-2]

*****
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*****

Hi Mike,
great choice! The opamp itself (and the module as well) is geared towards
non-inverting configuration, but it will work OK for you.

Obviously you don't want the inverting input shorted to ground, but proper
50 Ohm input impedance (as long as the other components - the detector and
the Olympus ADC - are 50 Ohm). Looks like easy fix.

Good luck!

zdenek


---------- Původní e-mail ----------
Od: Michael Giacomelli <[hidden email]>
Komu: [hidden email]
Datum: 6. 9. 2018 13:13:49
Předmět: Re: inverting photo detector polarity on a microscope
"*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Craig. It looks like I could modify the THS3120EVM to operate at a
gain of -1 by swapping out some resistors. The opamp itself is unity gain
stable.

Mike

On Thu, Sep 6, 2018 at 12:43 PM Craig Brideau <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> You'd still need to apply a 2V offset then come down from that. By the
time

> you splice in the bias offset you might as well have patched in an
> inverter.
> Craig
>
> On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > You could just record the data as is and invert the image afterwards?
> >
> > Mark B. Cannell. Ph.D. FRSNZ FISHR
> > Department of Physiology, Pharmacology & Neuroscience
> > School of Medical Sciences
> > University Walk
> > Bristol BS8 1TD
> >
> > [hidden email]
> >
> >
> >
> > On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
> > Allgeyer" <[hidden email] on behalf of [hidden email]

> >
> > wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> > posting.
> > *****
> >
> > Hi Mike,
> >
> > I have no commercial interest in either of the things I'm going to
> > mention
> > but they maybe of interest to you.
> >
> > First, there is a California based company, Pulse Research Lab, that
> > sells
> > a selection of high speed off the shelf comparators/sine wave
> > converters
> > that I've had success with for similar purposes. Their comparators
> are
> > fairly forgiving with what you connect as the input and you have a
> > number
> > of options for the corresponding output. You can browse their
> selection
> > here:
> >
> >
> >
> https://www.pulseresearchlab.com/collections/comparators-sinewave-

converters-1

> >
> > I'll also mention that on their comparators they have preset
> > thresholds but
> > you can also set an arbitrary threshold by applying a DC voltage to
> the
> > complementary input if you want to.
> >
> > Second, Picoquant also sells a few inverters and adaptors that might
> > work
> > for you. Specifically the SI100 is a passive inline pulse inverter
> and
> > might work for your application. You can browse their adapters,
> > splitters
> > and cables here:
> >
> >
> >
> https://www.picoquant.com/products/category/accessories/adapters-splitters

-cables-various-accessories-for-photon-counting-setups

> >
> > Hopefully this helps. Again, no commercial interest in either of
> these
> > companies. Just thought I'd mention them as possible options.
> >
> > Best,
> > Ed
> >
> > On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Hi,
> > >
> > > I would like to test out a new detector module in a commerical
> > Olympus
> > > confocal body. Unfortunately, the detector uses an inverting
> > amplifier
> > > (0->-1 V), while the Olympus assumes a non-inverting transimpedance
> > > amplifier (0->+1 V). I've spoken with them and there is no way to
> > change
> > > the A/D configuration on this specific model, and so I cannot use
> the
> > > detector as is.
> > >
> > > Is there a commerical solution available for adapting something
> like
> > this?
> > > I would like to avoid having to design and breadboard an inverting
> > buffer
> > > amplifier if I can avoid it.
> > >
> > > Thanks,
> > > Mike
> > >
> >
> >
> >
>

"

[Craig Brideau]

*****
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*****

I'm glad it was helpful. The evaluation boards are much more convenient
than making the circuit from scratch, but still give you some leeway for
customization. At unity it will also make a good buffer, as Zdenek's
comment on ensuring a 50 ohm load implies.
Craig

On Thu, Sep 6, 2018 at 11:11 AM Michael Giacomelli <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thanks Craig.  It looks like I could modify the  THS3120EVM to operate at a
> gain of -1 by swapping out some resistors.  The opamp itself is unity gain
> stable.
>
> Mike
>
> On Thu, Sep 6, 2018 at 12:43 PM Craig Brideau <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > You'd still need to apply a 2V offset then come down from that. By the
> time
> > you splice in the bias offset you might as well have patched in an
> > inverter.
> > Craig
> >
> > On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <[hidden email]
> >
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > You could just record the data as is and invert the image afterwards?
> > >
> > > Mark B. Cannell. Ph.D. FRSNZ FISHR
> > > Department of Physiology, Pharmacology & Neuroscience
> > > School of Medical Sciences
> > > University Walk
> > > Bristol BS8 1TD
> > >
> > > [hidden email]
> > >
> > >
> > >
> > > On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
> > > Allgeyer" <[hidden email] on behalf of
> [hidden email]
> > >
> > > wrote:
> > >
> > >     *****
> > >     To join, leave or search the confocal microscopy listserv, go to:
> > >     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >     Post images on http://www.imgur.com and include the link in your
> > > posting.
> > >     *****
> > >
> > >     Hi Mike,
> > >
> > >     I have no commercial interest in either of the things I'm going to
> > > mention
> > >     but they maybe of interest to you.
> > >
> > >     First, there is a California based company, Pulse Research Lab,
> that
> > > sells
> > >     a selection of high speed off the shelf comparators/sine wave
> > > converters
> > >     that I've had success with for similar purposes. Their comparators
> > are
> > >     fairly forgiving with what you connect as the input and you have a
> > > number
> > >     of options for the corresponding output. You can browse their
> > selection
> > >     here:
> > >
> > >
> > >
> >
> https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
> > >
> > >     I'll also mention that on their comparators they have preset
> > > thresholds but
> > >     you can also set an arbitrary threshold by applying a DC voltage to
> > the
> > >     complementary input if you want to.
> > >
> > >     Second, Picoquant also sells a few inverters and adaptors that
> might
> > > work
> > >     for you. Specifically the SI100 is a passive inline pulse inverter
> > and
> > >     might work for your application. You can browse their adapters,
> > > splitters
> > >     and cables here:
> > >
> > >
> > >
> >
> https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
> > >
> > >     Hopefully this helps. Again, no commercial interest in either of
> > these
> > >     companies. Just thought I'd mention them as possible options.
> > >
> > >     Best,
> > >     Ed
> > >
> > >     On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]>
> > > wrote:
> > >
> > >     > *****
> > >     > To join, leave or search the confocal microscopy listserv, go to:
> > >     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > >     > Post images on http://www.imgur.com and include the link in your
> > > posting.
> > >     > *****
> > >     >
> > >     > Hi,
> > >     >
> > >     > I would like to test out a new detector module in a commerical
> > > Olympus
> > >     > confocal body.  Unfortunately, the detector uses an inverting
> > > amplifier
> > >     > (0->-1 V), while the Olympus assumes a non-inverting
> transimpedance
> > >     > amplifier (0->+1 V).  I've spoken with them and there is no way
> to
> > > change
> > >     > the A/D configuration on this specific model, and so I cannot use
> > the
> > >     > detector as is.
> > >     >
> > >     > Is there a commerical solution available for adapting something
> > like
> > > this?
> > >     > I would like to avoid having to design and breadboard an
> inverting
> > > buffer
> > >     > amplifier if I can avoid it.
> > >     >
> > >     > Thanks,
> > >     > Mike
> > >     >
> > >
> > >
> > >
> >
>

[Mark Cannell-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Why a 2v offset, he said the output is 1->0 V while he wants 0->1. That means at no signal his input is saturated (1v) and goes toward zero...

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD
 
[hidden email]
 
 

On 6/09/18, 5:43 PM, "Confocal Microscopy List on behalf of Craig Brideau" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    You'd still need to apply a 2V offset then come down from that. By the time
    you splice in the bias offset you might as well have patched in an inverter.
    Craig
   
    On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <[hidden email]>
    wrote:
   
    > *****
    > To join, leave or search the confocal microscopy listserv, go to:
    > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    > Post images on http://www.imgur.com and include the link in your posting.
    > *****
    >
    > You could just record the data as is and invert the image afterwards?
    >
    > Mark B. Cannell. Ph.D. FRSNZ FISHR
    > Department of Physiology, Pharmacology & Neuroscience
    > School of Medical Sciences
    > University Walk
    > Bristol BS8 1TD
    >
    > [hidden email]
    >
    >
    >
    > On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
    > Allgeyer" <[hidden email] on behalf of [hidden email]>
    > wrote:
    >
    >     *****
    >     To join, leave or search the confocal microscopy listserv, go to:
    >     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >     Post images on http://www.imgur.com and include the link in your
    > posting.
    >     *****
    >
    >     Hi Mike,
    >
    >     I have no commercial interest in either of the things I'm going to
    > mention
    >     but they maybe of interest to you.
    >
    >     First, there is a California based company, Pulse Research Lab, that
    > sells
    >     a selection of high speed off the shelf comparators/sine wave
    > converters
    >     that I've had success with for similar purposes. Their comparators are
    >     fairly forgiving with what you connect as the input and you have a
    > number
    >     of options for the corresponding output. You can browse their selection
    >     here:
    >
    >
    > https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
    >
    >     I'll also mention that on their comparators they have preset
    > thresholds but
    >     you can also set an arbitrary threshold by applying a DC voltage to the
    >     complementary input if you want to.
    >
    >     Second, Picoquant also sells a few inverters and adaptors that might
    > work
    >     for you. Specifically the SI100 is a passive inline pulse inverter and
    >     might work for your application. You can browse their adapters,
    > splitters
    >     and cables here:
    >
    >
    > https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
    >
    >     Hopefully this helps. Again, no commercial interest in either of these
    >     companies. Just thought I'd mention them as possible options.
    >
    >     Best,
    >     Ed
    >
    >     On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]>
    > wrote:
    >
    >     > *****
    >     > To join, leave or search the confocal microscopy listserv, go to:
    >     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    >     > Post images on http://www.imgur.com and include the link in your
    > posting.
    >     > *****
    >     >
    >     > Hi,
    >     >
    >     > I would like to test out a new detector module in a commerical
    > Olympus
    >     > confocal body.  Unfortunately, the detector uses an inverting
    > amplifier
    >     > (0->-1 V), while the Olympus assumes a non-inverting transimpedance
    >     > amplifier (0->+1 V).  I've spoken with them and there is no way to
    > change
    >     > the A/D configuration on this specific model, and so I cannot use the
    >     > detector as is.
    >     >
    >     > Is there a commerical solution available for adapting something like
    > this?
    >     > I would like to avoid having to design and breadboard an inverting
    > buffer
    >     > amplifier if I can avoid it.
    >     >
    >     > Thanks,
    >     > Mike
    >     >
    >
    >
    >
   

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Yes, typo on my part, should be 1V bias. Looks like he found an inverting
amp out of the boards I suggested which can be set to a gain of -1, which
will avoid the need for any biasing anyway, except perhaps a small offset
to nullify any dark signal. A nice feature of many of these boards is they
usually come pre-loaded with components, but the offset/bias and gain
resistors are replaceable, or even actually potentiometers to allow for
easy adjustment for the users' conditions.

Craig

On Thu, Sep 6, 2018 at 2:53 PM Mark Cannell <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Why a 2v offset, he said the output is 1->0 V while he wants 0->1. That
> means at no signal his input is saturated (1v) and goes toward zero...
>
> Mark B. Cannell. Ph.D. FRSNZ FISHR
> Department of Physiology, Pharmacology & Neuroscience
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
>
> [hidden email]
>
>
>
> On 6/09/18, 5:43 PM, "Confocal Microscopy List on behalf of Craig
> Brideau" <[hidden email] on behalf of
> [hidden email]> wrote:
>
>     *****
>     To join, leave or search the confocal microscopy listserv, go to:
>     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     Post images on http://www.imgur.com and include the link in your
> posting.
>     *****
>
>     You'd still need to apply a 2V offset then come down from that. By the
> time
>     you splice in the bias offset you might as well have patched in an
> inverter.
>     Craig
>
>     On Wed, Sep 5, 2018 at 11:42 PM Mark Cannell <
> [hidden email]>
>     wrote:
>
>     > *****
>     > To join, leave or search the confocal microscopy listserv, go to:
>     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     > Post images on http://www.imgur.com and include the link in your
> posting.
>     > *****
>     >
>     > You could just record the data as is and invert the image afterwards?
>     >
>     > Mark B. Cannell. Ph.D. FRSNZ FISHR
>     > Department of Physiology, Pharmacology & Neuroscience
>     > School of Medical Sciences
>     > University Walk
>     > Bristol BS8 1TD
>     >
>     > [hidden email]
>     >
>     >
>     >
>     > On 6/09/18, 2:23 AM, "Confocal Microscopy List on behalf of Edward
>     > Allgeyer" <[hidden email] on behalf of
> [hidden email]>
>     > wrote:
>     >
>     >     *****
>     >     To join, leave or search the confocal microscopy listserv, go to:
>     >     http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     >     Post images on http://www.imgur.com and include the link in your
>     > posting.
>     >     *****
>     >
>     >     Hi Mike,
>     >
>     >     I have no commercial interest in either of the things I'm going
> to
>     > mention
>     >     but they maybe of interest to you.
>     >
>     >     First, there is a California based company, Pulse Research Lab,
> that
>     > sells
>     >     a selection of high speed off the shelf comparators/sine wave
>     > converters
>     >     that I've had success with for similar purposes. Their
> comparators are
>     >     fairly forgiving with what you connect as the input and you have
> a
>     > number
>     >     of options for the corresponding output. You can browse their
> selection
>     >     here:
>     >
>     >
>     >
> https://www.pulseresearchlab.com/collections/comparators-sinewave-converters-1
>     >
>     >     I'll also mention that on their comparators they have preset
>     > thresholds but
>     >     you can also set an arbitrary threshold by applying a DC voltage
> to the
>     >     complementary input if you want to.
>     >
>     >     Second, Picoquant also sells a few inverters and adaptors that
> might
>     > work
>     >     for you. Specifically the SI100 is a passive inline pulse
> inverter and
>     >     might work for your application. You can browse their adapters,
>     > splitters
>     >     and cables here:
>     >
>     >
>     >
> https://www.picoquant.com/products/category/accessories/adapters-splitters-cables-various-accessories-for-photon-counting-setups
>     >
>     >     Hopefully this helps. Again, no commercial interest in either of
> these
>     >     companies. Just thought I'd mention them as possible options.
>     >
>     >     Best,
>     >     Ed
>     >
>     >     On Wed, Sep 5, 2018 at 12:58 PM Michael Giacomelli <[hidden email]
> >
>     > wrote:
>     >
>     >     > *****
>     >     > To join, leave or search the confocal microscopy listserv, go
> to:
>     >     > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>     >     > Post images on http://www.imgur.com and include the link in
> your
>     > posting.
>     >     > *****
>     >     >
>     >     > Hi,
>     >     >
>     >     > I would like to test out a new detector module in a commerical
>     > Olympus
>     >     > confocal body.  Unfortunately, the detector uses an inverting
>     > amplifier
>     >     > (0->-1 V), while the Olympus assumes a non-inverting
> transimpedance
>     >     > amplifier (0->+1 V).  I've spoken with them and there is no
> way to
>     > change
>     >     > the A/D configuration on this specific model, and so I cannot
> use the
>     >     > detector as is.
>     >     >
>     >     > Is there a commerical solution available for adapting
> something like
>     > this?
>     >     > I would like to avoid having to design and breadboard an
> inverting
>     > buffer
>     >     > amplifier if I can avoid it.
>     >     >
>     >     > Thanks,
>     >     > Mike
>     >     >
>     >
>     >
>     >
>
>
>