last seats for free - Diaspro Lab School on Super resolution in Genoa, Istituto Italiano di Tecnologia

[Alberto Diaspro]

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Dear
the Advanced practical School on Super resolution at the Italian Institute of Technology will take place from December 12th to December 16th.
We have few extra seats (3) for the practical sessions. The opening  lecture will be on Dec 12th, Italian Institute of Technology, Diaspro Lab, 2 p.m. Opening Lecture is open without registration need.

Opening Lecture by Peter Saggau on Advanced Optical Imaging for Reverse-Engineering the Brain. Lectures on Super resolution Methods and Nanoscopy based on targeted and stochastic readout (IML-SPIM, FPALM, STORM, STED, GSDIM etc..) will be given by Paolo Bianchini, Benjamin Harke, Francesca Cella Zanacchi and Giuseppe Vicidomini with insets by Silvia Galiani and Zeno Lavagnino.

Reference books are “Nanoscopy and Multidimensional Optical Fluorescence Microscopy”, Taylor &Francis Group, A Chapman & Hall Book Crc Press (2010), pp.448; “Optical Fluorescence Microscopy: from the spectral to the nano dimension”, Springer-Verlag, Berlin and Heidelberg GmbH & Co. K., (2010), pp. 256; “Confocal and Two- Photon Microscopy: Foundations, Applications and Advances”, Wiley-Liss (2001), pp. 580.

Recent recent papers by Diaspro lab members:

1) A. Diaspro, G. Chirico, M. Collini, “Two-photon fluorescence excitation and related techniques in biological microscopy”, Q. Rev.
Biophys 38(2), 97-166 (2005) DOI:10.1017/S0033583505004129.

2) A. Palamidessi, E. Frittoli, M. Garré, M. Faretta, M. Mione, I. Testa, A. Diaspro, L. Lanzetti, G. Scita, P. P. D. Fiore, “Endocytic
trafficking of Rac is required for the spatial restriction of signaling in cell migration”, Cell, 134(1), 135-147 (2008) DOI 10.1016/j.cell.2008.05.034.

3) P. Bianchini, A. Diaspro, "Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues."  Journal of Biophotonics, 1(6), 443–450. (2008) doi:10.1002/jbio.200810060

4) P.Bianchini, A.Diaspro, "Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam" J.Microscopy (OXF), in press (2011).

5) G. Vicidomini, G. Moneron, K.Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S.W. Hell.  "Sharper low-power STED nanoscopy by time gating." Nature Methods, 8(7), 571–573. (2011) doi:10.1038/nmeth.1624

6) B. Harke, J. Keller,  C.K. Ullal, V. Westphal, A. Schönle, S.W. Hell. "Resolution scaling in STED microscopy"  Optics express, 16(6), 4154–4162 (2008).

7) F. Cella Zanacchi, Z. Lavagnino, M. Perrone Donnorso, A. Del Bue, L. Furia, M. Faretta, A. Diaspro "Live-cell 3D super-resolution imaging in thick biological samples."  Nature Methods. (2011)  8(12), 1047–1049. doi:10.1038/nmeth.1744

8) P.P. Mondal, A. Diaspro "Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy." Nature  Sci. Rep. 1, 149; (2011) DOI:10.1038/ srep00149.


Please, send a simple e-mail to [hidden email] subject: extra seats IIT School with your name and institution, you will receive a prompt reply.

All the best
Alby


next year do not miss www.owls2012.org

[mmodel]

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Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!

Mike Model

[Martin Wessendorf-2]

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Dear Mike--

On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:

> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!

I'm not sure I'm getting the concept--do you want an objective that
*needs* to be in contact with the specimen to be in focus?  --It might
help me if you explained a bit more about what you're trying to do.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[mmodel]

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Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Thursday, December 08, 2011 4:27 PM
To: [hidden email]
Subject: Re: small and variable working distance

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Dear Mike--

On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:

> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!

I'm not sure I'm getting the concept--do you want an objective that
*needs* to be in contact with the specimen to be in focus?  --It might
help me if you explained a bit more about what you're trying to do.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Guy Cox-2]

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These are known as solid immersion lenses, and they certainly do exist.  From the little that I understand the idea is that the lens is within the evanescent field so that the refractive index appears to be continuous to the sample.  I'm sure there optics people on this list who could give a better explanation.   Whether you could get one to fit a standard microscope is a different question entirely, but if you are working on an optical bench I'd imagine the usual suspects could oblige.  

                                 Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL
Sent: Friday, 9 December 2011 8:47 AM
To: [hidden email]
Subject: Re: small and variable working distance

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Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Thursday, December 08, 2011 4:27 PM
To: [hidden email]
Subject: Re: small and variable working distance

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Dear Mike--

On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:

> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!

I'm not sure I'm getting the concept--do you want an objective that
*needs* to be in contact with the specimen to be in focus?  --It might
help me if you explained a bit more about what you're trying to do.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Martin Wessendorf-2]

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Dear Mike--

How close do you need to get and what kind of resolution do you need?
And in what medium?  Air?  Water?  Oil?

Good luck!

Martin

On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:

> Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
>> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might
> help me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Arne Seitz]

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Hi Mike,
if you want to bring the objective as close to the object as possible maybe Scanning Near-field Optical microscopy (SNOM) could do the job.

Cheers Arne

---------------------------------------------------------------
Dr. Arne Seitz
Head of Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 15, AI 0241
CH-1015 Lausanne

Phone: [phone]
Fax:      [phone]
http://biop.epfl.ch/
---------------------------------------------------------------

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of MODEL,
> MICHAEL
> Sent: jeudi 8 décembre 2011 22:47
> To: [hidden email]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Martin - that's correct, I want to bring the objective very close to the
> specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Martin
> Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
> > Dear Confocal Microscopists - Does anyone of an objective with variable,
> down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might help
> me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

[mmodel]

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To join, leave or search the confocal microscopy listserv, go to:
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20-30 microns in water. I doubt such lenses exist but who knows... Thanks!

Mike

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Martin Wessendorf [[hidden email]]
Sent: Thursday, December 08, 2011 9:20 PM
To: [hidden email]
Subject: Re: small and variable working distance

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Dear Mike--

How close do you need to get and what kind of resolution do you need?
And in what medium?  Air?  Water?  Oil?

Good luck!

Martin

On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:

> Hi Martin - that's correct, I want to bring the objective very close to the specimen, almost touching it.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
> Sent: Thursday, December 08, 2011 4:27 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
> Dear Mike--
>
> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>
>> Dear Confocal Microscopists - Does anyone of an objective with variable, down to zero, working distance? Thanks!
>
> I'm not sure I'm getting the concept--do you want an objective that
> *needs* to be in contact with the specimen to be in focus?  --It might
> help me if you explained a bit more about what you're trying to do.
>
> Thanks--
>
> Martin Wessendorf

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Jeremy Adler-4]

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Out of interest, is the limitation that the water layer is only 20-30  
microns thick or that the objective really needs to be that close ?

a 20-30 micron thick layer of water can easily by sandwiched between a  
slide and a coverslip using microspheres as spaces. Then image with a  
water objective that works thru a coverslip.




Quoting "MODEL, MICHAEL" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> 20-30 microns in water. I doubt such lenses exist but who knows... Thanks!
>
> Mike
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On  
> Behalf Of Martin Wessendorf [[hidden email]]
> Sent: Thursday, December 08, 2011 9:20 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mike--
>
> How close do you need to get and what kind of resolution do you need?
> And in what medium?  Air?  Water?  Oil?
>
> Good luck!
>
> Martin
>
> On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:
>
>> Hi Martin - that's correct, I want to bring the objective very  
>> close to the specimen, almost touching it.
>>
>> -----Original Message-----
>> From: Confocal Microscopy List  
>> [mailto:[hidden email]] On Behalf Of Martin  
>> Wessendorf
>> Sent: Thursday, December 08, 2011 4:27 PM
>> To: [hidden email]
>> Subject: Re: small and variable working distance
>> Dear Mike--
>>
>> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>>
>>> Dear Confocal Microscopists - Does anyone of an objective with  
>>> variable, down to zero, working distance? Thanks!
>>
>> I'm not sure I'm getting the concept--do you want an objective that
>> *needs* to be in contact with the specimen to be in focus?  --It might
>> help me if you explained a bit more about what you're trying to do.
>>
>> Thanks--
>>
>> Martin Wessendorf
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607

[mmodel]

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That's right, that's how deep the water layer should be, and we do use all kinds of sandwiches, even without microspheres. But when you need to continuously exchange solutions, especially while keeping everything at 37C, it becomes a little inconvenient. A Bioptechs flow chamber with a thin spacer works well when cells can be grown on glass, but for cells on a plastic dish the simplest thing would be simply to bring the objective close enough to the bottom. The working distance of common water-immersion objectives is too large for that.

Mike
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Jeremy Adler [[hidden email]]
Sent: Friday, December 09, 2011 7:22 AM
To: [hidden email]
Subject: Re: small and variable working distance

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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Out of interest, is the limitation that the water layer is only 20-30
microns thick or that the objective really needs to be that close ?

a 20-30 micron thick layer of water can easily by sandwiched between a
slide and a coverslip using microspheres as spaces. Then image with a
water objective that works thru a coverslip.




Quoting "MODEL, MICHAEL" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> 20-30 microns in water. I doubt such lenses exist but who knows... Thanks!
>
> Mike
>
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Martin Wessendorf [[hidden email]]
> Sent: Thursday, December 08, 2011 9:20 PM
> To: [hidden email]
> Subject: Re: small and variable working distance
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Mike--
>
> How close do you need to get and what kind of resolution do you need?
> And in what medium?  Air?  Water?  Oil?
>
> Good luck!
>
> Martin
>
> On 12/8/2011 3:47 PM, MODEL, MICHAEL wrote:
>
>> Hi Martin - that's correct, I want to bring the objective very
>> close to the specimen, almost touching it.
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[hidden email]] On Behalf Of Martin
>> Wessendorf
>> Sent: Thursday, December 08, 2011 4:27 PM
>> To: [hidden email]
>> Subject: Re: small and variable working distance
>> Dear Mike--
>>
>> On 12/8/2011 3:05 PM, MODEL, MICHAEL wrote:
>>
>>> Dear Confocal Microscopists - Does anyone of an objective with
>>> variable, down to zero, working distance? Thanks!
>>
>> I'm not sure I'm getting the concept--do you want an objective that
>> *needs* to be in contact with the specimen to be in focus?  --It might
>> help me if you explained a bit more about what you're trying to do.
>>
>> Thanks--
>>
>> Martin Wessendorf
>
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

Jeremy Adler
IGP
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607

[Alberto Diaspro]

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Dear friends
full program is available at www.lambs.it
Ciao
Alby


Il giorno 08/dic/2011, alle ore 09:57, Alberto Diaspro ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear
> the Advanced practical School on Super resolution at the Italian Institute of Technology will take place from December 12th to December 16th.
> We have few extra seats (3) for the practical sessions. The opening  lecture will be on Dec 12th, Italian Institute of Technology, Diaspro Lab, 2 p.m. Opening Lecture is open without registration need.
>
> Opening Lecture by Peter Saggau on Advanced Optical Imaging for Reverse-Engineering the Brain. Lectures on Super resolution Methods and Nanoscopy based on targeted and stochastic readout (IML-SPIM, FPALM, STORM, STED, GSDIM etc..) will be given by Paolo Bianchini, Benjamin Harke, Francesca Cella Zanacchi and Giuseppe Vicidomini with insets by Silvia Galiani and Zeno Lavagnino.
>
> Reference books are “Nanoscopy and Multidimensional Optical Fluorescence Microscopy”, Taylor &Francis Group, A Chapman & Hall Book Crc Press (2010), pp.448; “Optical Fluorescence Microscopy: from the spectral to the nano dimension”, Springer-Verlag, Berlin and Heidelberg GmbH & Co. K., (2010), pp. 256; “Confocal and Two- Photon Microscopy: Foundations, Applications and Advances”, Wiley-Liss (2001), pp. 580.
>
> Recent recent papers by Diaspro lab members:
>
> 1) A. Diaspro, G. Chirico, M. Collini, “Two-photon fluorescence excitation and related techniques in biological microscopy”, Q. Rev.
> Biophys 38(2), 97-166 (2005) DOI:10.1017/S0033583505004129.
>
> 2) A. Palamidessi, E. Frittoli, M. Garré, M. Faretta, M. Mione, I. Testa, A. Diaspro, L. Lanzetti, G. Scita, P. P. D. Fiore, “Endocytic
> trafficking of Rac is required for the spatial restriction of signaling in cell migration”, Cell, 134(1), 135-147 (2008) DOI 10.1016/j.cell.2008.05.034.
>
> 3) P. Bianchini, A. Diaspro, "Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues."  Journal of Biophotonics, 1(6), 443–450. (2008) doi:10.1002/jbio.200810060
>
> 4) P.Bianchini, A.Diaspro, "Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam" J.Microscopy (OXF), in press (2011).
>
> 5) G. Vicidomini, G. Moneron, K.Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S.W. Hell.  "Sharper low-power STED nanoscopy by time gating." Nature Methods, 8(7), 571–573. (2011) doi:10.1038/nmeth.1624
>
> 6) B. Harke, J. Keller,  C.K. Ullal, V. Westphal, A. Schönle, S.W. Hell. "Resolution scaling in STED microscopy"  Optics express, 16(6), 4154–4162 (2008).
>
> 7) F. Cella Zanacchi, Z. Lavagnino, M. Perrone Donnorso, A. Del Bue, L. Furia, M. Faretta, A. Diaspro "Live-cell 3D super-resolution imaging in thick biological samples."  Nature Methods. (2011)  8(12), 1047–1049. doi:10.1038/nmeth.1744
>
> 8) P.P. Mondal, A. Diaspro "Simultaneous multilayer scanning and detection for multiphoton fluorescence microscopy." Nature  Sci. Rep. 1, 149; (2011) DOI:10.1038/ srep00149.
>
>
> Please, send a simple e-mail to [hidden email] subject: extra seats IIT School with your name and institution, you will receive a prompt reply.
>
> All the best
> Alby
>
>
> next year do not miss www.owls2012.org