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Hi Matthew-
Electroporation of chick embryos is tricky but doable and many labs get it going. For an example of this type of experiment, there are some useful references:
PMID: 16312219
and a more recent one:
PMID: 18383543
(sorry, these are subscription access only, so am including PubMed IDs as opposed direct links-- go open access!)
These discuss two different ways of introducing fluorophores into sections-- there are many more.
We've recently been working with chick embryo sections electroporated with various FP fusions and directly following cell division and differentitation:
http://dev.biologists.org/cgi/content/full/134/10/1943
If you want any help with these methods, just contact me directly.
Cheers,
Jason
On Mon, Jun 2, 2008 at 5:41 AM, Matthew Smith <
[hidden email]> wrote:
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hello. i'm trying to get high detail live confocal images of cross sections
of chicken embryos. i've been adding a gfp vector, but the transfection
method is giving me a hard time. i was hoping someone might have
suggestions for alternatives. general cell morphology is good enough.
thank you, matthew
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