looking for a second-hand 2 photon upright microscope
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have a colleague who is looking for a second-hand 2 photon upright microscope. Any information including reputable websites that sell used microscopes greatly appreciated; please contact offline. Thank you! ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello All, We are in the process of purchasing a EMCCD camera for our microscope to look at very low levels of fluorescence. In this context, we evaluated the following cameras 1. Photometrics Evolve 512 2. Andor iXon X3 DU897. Both of them performed similarly though the Andor had less background noise. However, the Andor does not have an on/off switch and the fan stays on unless we shut down the computer. So the question to all the folks who have used Andor before is that have you had issues with this kind of system. Any feedback will be great for us to make a decision. Thanks, -Prabhakar |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On Tue, Mar 22, 2011 at 3:26 PM, B. Prabhakar Pandian <[hidden email]> wrote: > However, the Andor does not have an on/off switch and the fan stays on > unless we shut down the computer. > > I recently asked our local Andor rep, Charles Fanghella, about this issue and his response may be of interest: ******************** Then fan is powered by the PCI interface card and cable, so as long as the PC is on the fan will have power. It actually is not cooling the camera down to your set temperature just keeping the electronics cool. There is no problem with letting it run continuously, as the factory has a system going for 7 sevens years round the clock. If you want to leave the PC on and stop the fan just disconnect the interface cable from the head of the camera. However, connect and disconnect only when you have closed your software *Regards;* *Charles Fanghella* Regional Sales Engineer **************** We have an iXon 860 and have been happy with it. Regards, Matt -- Matthew Nicholas Medical Scientist Training Program Student Laboratory of Arne Gennerich Department of Anatomy and Structural Biology Albert Einstein College of Medicine Forchheimer Building, Room 628 1300 Morris Park Avenue Bronx, New York 10461 718.430.3446 [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Am I clear in understanding here that it is OK to hot plug/unplug your Andor at the camera head? Cheers Neil > Date: Tue, 22 Mar 2011 16:26:36 -0400 > From: [hidden email] > Subject: Re: EMCCD Camera > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > On Tue, Mar 22, 2011 at 3:26 PM, B. Prabhakar Pandian <[hidden email]> wrote: > > > However, the Andor does not have an on/off switch and the fan stays on > > unless we shut down the computer. > > > > > I recently asked our local Andor rep, Charles Fanghella, about this issue > and his response may be of interest: > > ******************** > > Then fan is powered by the PCI interface card and cable, so as long as the > PC is on the fan will have power. It actually is not cooling the camera down > to your set temperature just keeping the electronics cool. There is no > problem with letting it run continuously, as the factory has a system going > for 7 sevens years round the clock. If you want to leave the PC on and stop > the fan just disconnect the interface cable from the head of the camera. > However, connect and disconnect only when you have closed your software > > > > *Regards;* > > *Charles Fanghella* > Regional Sales Engineer > **************** > > > We have an iXon 860 and have been happy with it. > > Regards, > Matt > > -- > Matthew Nicholas > Medical Scientist Training Program Student > Laboratory of Arne Gennerich > Department of Anatomy and Structural Biology > Albert Einstein College of Medicine > Forchheimer Building, Room 628 > 1300 Morris Park Avenue > Bronx, New York 10461 > 718.430.3446 > [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** On Tue, Mar 22, 2011 at 4:31 PM, Neil Kad <[hidden email]> wrote: > > Am I clear in understanding here that it is OK to hot plug/unplug your > Andor at the camera head? > > As I understand it, yes (someone please correct me if you have experience to the contrary). However, I have not done so personally. My main concern is actually physical wear on the connector due to constantly plugging/unplugging it. Our microscope is in pretty frequent use, so we leave the iXon on more or less constantly. When it will not be used for more than a day or so, we usually shut down the PC (and therefore the camera). Best regards, Matt -- Matthew Nicholas Medical Scientist Training Program Student Laboratory of Arne Gennerich Department of Anatomy and Structural Biology Albert Einstein College of Medicine Forchheimer Building, Room 628 1300 Morris Park Avenue Bronx, New York 10461 718.430.3446 [hidden email] |
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In reply to this post by B. Prabhakar Pandian
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I have an Andor 897 on a system in my core facility. The system (computer) is on 10-12hrs a day coming up on 3yrs and I haven't had any fan/cooling issues. We shut down the system at night and I have taught users to shut down the computer. (also had Andor EMCCDs in past labs and they worked well) For hotswaping: My understanding is the computer can be on, but the software controlling the camera needs to be off. But perhaps we should get confirmation from an Andor rep. -Lisa --------------------------------------- Lisa Cameron, Ph.D. Director of Confocal and Light Microscopy Dana Farber Cancer Institute Boston, MA 02215 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian Sent: Tuesday, March 22, 2011 3:26 PM To: [hidden email] Subject: [CONFOCALMICROSCOPY] EMCCD Camera ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello All, We are in the process of purchasing a EMCCD camera for our microscope to look at very low levels of fluorescence. In this context, we evaluated the following cameras 1. Photometrics Evolve 512 2. Andor iXon X3 DU897. Both of them performed similarly though the Andor had less background noise. However, the Andor does not have an on/off switch and the fan stays on unless we shut down the computer. So the question to all the folks who have used Andor before is that have you had issues with this kind of system. Any feedback will be great for us to make a decision. Thanks, -Prabhakar The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I heard back from Andor at their USA Connecticut Office: "As long as the software is not grabbing control of the camera, you can remove the white cable from the camera or the PC. When the power from the PCI bus is no longer getting to the camera, the fan will not run. Of course, if you plan to use the computer without the camera, it is best practice to disconnect it before starting but if you need to disconnect while the PC power is on, it will not damage the hardware. If you try to disconnect while Andor iQ software is controlling the camera, it can cause errors so do take due caution not to do this. In addition, we have designed our newer PCI-Express CCI-24 card to turn the fan off when the camera is not in use and the acquisition software is exited, even if the PC is on. -Tom Nunn" Prabhakar, perhaps you can request this new PCI-express card at purchase - Lisa --------------------------------------- Lisa Cameron, Ph.D. Director of Confocal and Light Microscopy Dana Farber Cancer Institute Boston, MA -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian Sent: Tuesday, March 22, 2011 3:26 PM To: [hidden email] Subject: [CONFOCALMICROSCOPY] EMCCD Camera ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello All, We are in the process of purchasing a EMCCD camera for our microscope to look at very low levels of fluorescence. In this context, we evaluated the following cameras 1. Photometrics Evolve 512 2. Andor iXon X3 DU897. Both of them performed similarly though the Andor had less background noise. However, the Andor does not have an on/off switch and the fan stays on unless we shut down the computer. So the question to all the folks who have used Andor before is that have you had issues with this kind of system. Any feedback will be great for us to make a decision. Thanks, -Prabhakar The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. |
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In reply to this post by B. Prabhakar Pandian
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Prabhakar, I have used both these cameras and in my opinion, the Evolve is a much superior camera with the photoelectron info readout ability and plug and play format with no need for a framegrabber at all that to me there is no question. Plus the Evolve self calibrates so it is set up for your system once it has been connected. I love this camera!! Loralei On Tue, Mar 22, 2011 at 12:26 PM, B. Prabhakar Pandian <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hello All, > We are in the process of purchasing a EMCCD camera for > our microscope to look at very low levels of fluorescence. In this context, > we evaluated the following cameras > > 1. Photometrics Evolve 512 > > 2. Andor iXon X3 DU897. > > Both of them performed similarly though the Andor had less background > noise. However, the Andor does not have an on/off switch and the fan stays > on unless we shut down the computer. So the question to all the folks who > have used Andor before is that have you had issues with this kind of system. > > Any feedback will be great for us to make a decision. > > Thanks, > > -Prabhakar > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Loralei, Thanks for the information. Could you please clarify with some details as to your reason on the superiority of the camera. We had couple of issues with the Evolve. 1. The main controller wires run off the front of the camera and there is very high probability that because of this mode, the wires can go bad in the long run. 2. Andor had a much higher signal/noise ratio for our samples of about 10nM. 3. In Windows 7, the Firewire used by Evolve has the last preference in priority. I would love to hear your comments on your usage. Thanks, -Prabhakar On 3/22/2011 5:16 PM, Loralei Dewe wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Prabhakar, > > I have used both these cameras and in my opinion, the Evolve is a much > superior camera with the photoelectron info readout ability and plug and > play format with no need for a framegrabber at all that to me there is no > question. Plus the Evolve self calibrates so it is set up for your > system once it has been connected. I love this camera!! > > Loralei > > On Tue, Mar 22, 2011 at 12:26 PM, B. Prabhakar Pandian<[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hello All, >> We are in the process of purchasing a EMCCD camera for >> our microscope to look at very low levels of fluorescence. In this context, >> we evaluated the following cameras >> >> 1. Photometrics Evolve 512 >> >> 2. Andor iXon X3 DU897. >> >> Both of them performed similarly though the Andor had less background >> noise. However, the Andor does not have an on/off switch and the fan stays >> on unless we shut down the computer. So the question to all the folks who >> have used Andor before is that have you had issues with this kind of system. >> >> Any feedback will be great for us to make a decision. >> >> Thanks, >> >> -Prabhakar >> |
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In reply to this post by Matthew Nicholas-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Matthew Nicholas <[hidden email]> writes: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > On Tue, Mar 22, 2011 at 3:26 PM, B. Prabhakar Pandian <[hidden email]> wrote: > >> However, the Andor does not have an on/off switch and the fan stays on >> unless we shut down the computer. >> >> > I recently asked our local Andor rep, Charles Fanghella, about this issue > and his response may be of interest: No mater what Andor says this is a mad piece of design. Fans are mechanical and do fail. We have had a fan ware out and the camera had to go back to Andor, so now we shut down the PC which isn't the most convenient either. I think that fact that new version don't have this "feature" demonstrates that even Andor think it is a bad idea. Ian -- Ian Dobbie Micron Imaging Facility Manager, Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU Tel: 01865 613323 Email: [hidden email] |
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