looking for used fluorescence microscope

classic Classic list List threaded Threaded
8 messages Options
Martin Schattat Martin Schattat
Reply | Threaded
Open this post in threaded view
|

looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear List members,

a few months ago I started my own plant cell biology lab in Halle (Saale), Germany. I intend to do a microscopy based screen, which will probably occupy the a lot of microscopy time on the institute microscope. Because I don't want to cause inconvenience for my coworkers and for reasons of imaging independence I am looking for a used wide field fluorescence microscope. A standard wide field system like the Axioskop2 series of Zeiss as upright or even inverted design is sufficient for my purpose. I have some funding which I could use for purchasing and adapting it for my needs.

Has some one a good idea where I could find a used wide field system? I am also happy about any other suggestion regarding sources of used microscope equipment.

Thanks a lot

Martin
 
-------------------------------------------------------------------
Dr. Martin Schattat
Martin-Luther-Universität Halle-Wittenberg
Institutsbereich Pflanzenphysiologie
Weinbergweg 10, 06120 Halle (Saale), Germany

Tel.:     +49 345 5526286
Email: [hidden email]
Chris Tully Chris Tully
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Martin,

I would suggest getting in touch with as many microscope sales reps and
dealership owners as you can. Some dealers will buy up used microscopes
when companies close or labs upgrade, and then resell them at a fraction of
the new price. In my experience this is a word of mouth type business and
not one that is easily found on the internet.  The one person who I know
does this is based in Utah, USA and I have lost contact with him so I doubt
that he will be much help.

Good Luck,

Chris Tully
Microscopy and Image Analysis Expert
[hidden email]
240-475-9753 (c)

[image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>


On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear List members,
>
> a few months ago I started my own plant cell biology lab in Halle (Saale),
> Germany. I intend to do a microscopy based screen, which will probably
> occupy the a lot of microscopy time on the institute microscope. Because I
> don't want to cause inconvenience for my coworkers and for reasons of
> imaging independence I am looking for a used wide field fluorescence
> microscope. A standard wide field system like the Axioskop2 series of Zeiss
> as upright or even inverted design is sufficient for my purpose. I have
> some funding which I could use for purchasing and adapting it for my needs.
>
> Has some one a good idea where I could find a used wide field system? I am
> also happy about any other suggestion regarding sources of used microscope
> equipment.
>
> Thanks a lot
>
> Martin
>
> -------------------------------------------------------------------
> Dr. Martin Schattat
> Martin-Luther-Universität Halle-Wittenberg
> Institutsbereich Pflanzenphysiologie
> Weinbergweg 10, 06120 Halle (Saale), Germany
>
> Tel.:     +49 345 5526286
> Email: [hidden email]
>
Craig Brideau Craig Brideau
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You can also get kit-based solutions from a lot of vendors.  Scientifica
and ThorLabs have some really nice little widefield systems at competitive
prices.  Scientifica has this tiny little modular hexagonal stack that
doesn't look like a microscope, but does a heck of a lot of widefield
imaging techniques.  If you can forgo eyepieces and live with seeing
everything on a monitor it might be an interesting (and cost effective) way
to go.

http://www.scientifica.uk.com/products/imic-digital-imaging-platform

Check it out, even if just to see the pictures.  It looks... different.

Craig


On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Martin,
>
> I would suggest getting in touch with as many microscope sales reps and
> dealership owners as you can. Some dealers will buy up used microscopes
> when companies close or labs upgrade, and then resell them at a fraction of
> the new price. In my experience this is a word of mouth type business and
> not one that is easily found on the internet.  The one person who I know
> does this is based in Utah, USA and I have lost contact with him so I doubt
> that he will be much help.
>
> Good Luck,
>
> Chris Tully
> Microscopy and Image Analysis Expert
> [hidden email]
> 240-475-9753 (c)
>
> [image: View my profile on LinkedIn]<
> http://www.linkedin.com/in/christully/>
>
>
> On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear List members,
> >
> > a few months ago I started my own plant cell biology lab in Halle
> (Saale),
> > Germany. I intend to do a microscopy based screen, which will probably
> > occupy the a lot of microscopy time on the institute microscope. Because
> I
> > don't want to cause inconvenience for my coworkers and for reasons of
> > imaging independence I am looking for a used wide field fluorescence
> > microscope. A standard wide field system like the Axioskop2 series of
> Zeiss
> > as upright or even inverted design is sufficient for my purpose. I have
> > some funding which I could use for purchasing and adapting it for my
> needs.
> >
> > Has some one a good idea where I could find a used wide field system? I
> am
> > also happy about any other suggestion regarding sources of used
> microscope
> > equipment.
> >
> > Thanks a lot
> >
> > Martin
> >
> > -------------------------------------------------------------------
> > Dr. Martin Schattat
> > Martin-Luther-Universität Halle-Wittenberg
> > Institutsbereich Pflanzenphysiologie
> > Weinbergweg 10, 06120 Halle (Saale), Germany
> >
> > Tel.:     +49 345 5526286
> > Email: [hidden email]
> >
>
Rosemary.White Rosemary.White
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

That looks really cool! Has anyone got one in their lab or at least tried one with their favourite tissue?

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Craig Brideau [[hidden email]]
Sent: Tuesday, 15 January 2013 6:40 a.m.
To: [hidden email]
Subject: Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You can also get kit-based solutions from a lot of vendors.  Scientifica
and ThorLabs have some really nice little widefield systems at competitive
prices.  Scientifica has this tiny little modular hexagonal stack that
doesn't look like a microscope, but does a heck of a lot of widefield
imaging techniques.  If you can forgo eyepieces and live with seeing
everything on a monitor it might be an interesting (and cost effective) way
to go.

http://www.scientifica.uk.com/products/imic-digital-imaging-platform

Check it out, even if just to see the pictures.  It looks... different.

Craig


On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Martin,
>
> I would suggest getting in touch with as many microscope sales reps and
> dealership owners as you can. Some dealers will buy up used microscopes
> when companies close or labs upgrade, and then resell them at a fraction of
> the new price. In my experience this is a word of mouth type business and
> not one that is easily found on the internet.  The one person who I know
> does this is based in Utah, USA and I have lost contact with him so I doubt
> that he will be much help.
>
> Good Luck,
>
> Chris Tully
> Microscopy and Image Analysis Expert
> [hidden email]
> 240-475-9753 (c)
>
> [image: View my profile on LinkedIn]<
> http://www.linkedin.com/in/christully/>
>
>
> On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear List members,
> >
> > a few months ago I started my own plant cell biology lab in Halle
> (Saale),
> > Germany. I intend to do a microscopy based screen, which will probably
> > occupy the a lot of microscopy time on the institute microscope. Because
> I
> > don't want to cause inconvenience for my coworkers and for reasons of
> > imaging independence I am looking for a used wide field fluorescence
> > microscope. A standard wide field system like the Axioskop2 series of
> Zeiss
> > as upright or even inverted design is sufficient for my purpose. I have
> > some funding which I could use for purchasing and adapting it for my
> needs.
> >
> > Has some one a good idea where I could find a used wide field system? I
> am
> > also happy about any other suggestion regarding sources of used
> microscope
> > equipment.
> >
> > Thanks a lot
> >
> > Martin
> >
> > -------------------------------------------------------------------
> > Dr. Martin Schattat
> > Martin-Luther-Universität Halle-Wittenberg
> > Institutsbereich Pflanzenphysiologie
> > Weinbergweg 10, 06120 Halle (Saale), Germany
> >
> > Tel.:     +49 345 5526286
> > Email: [hidden email]
> >
>
Loralei Dewe-3 Loralei Dewe-3
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

In reply to this post by Craig Brideau
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

It's a TILL system and is far from inexpensive!!

Loralei
On Jan 14, 2013 11:41 AM, "Craig Brideau" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> You can also get kit-based solutions from a lot of vendors.  Scientifica
> and ThorLabs have some really nice little widefield systems at competitive
> prices.  Scientifica has this tiny little modular hexagonal stack that
> doesn't look like a microscope, but does a heck of a lot of widefield
> imaging techniques.  If you can forgo eyepieces and live with seeing
> everything on a monitor it might be an interesting (and cost effective) way
> to go.
>
> http://www.scientifica.uk.com/products/imic-digital-imaging-platform
>
> Check it out, even if just to see the pictures.  It looks... different.
>
> Craig
>
>
> On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Martin,
> >
> > I would suggest getting in touch with as many microscope sales reps and
> > dealership owners as you can. Some dealers will buy up used microscopes
> > when companies close or labs upgrade, and then resell them at a fraction
> of
> > the new price. In my experience this is a word of mouth type business and
> > not one that is easily found on the internet.  The one person who I know
> > does this is based in Utah, USA and I have lost contact with him so I
> doubt
> > that he will be much help.
> >
> > Good Luck,
> >
> > Chris Tully
> > Microscopy and Image Analysis Expert
> > [hidden email]
> > 240-475-9753 (c)
> >
> > [image: View my profile on LinkedIn]<
> > http://www.linkedin.com/in/christully/>
> >
> >
> > On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
> > [hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Dear List members,
> > >
> > > a few months ago I started my own plant cell biology lab in Halle
> > (Saale),
> > > Germany. I intend to do a microscopy based screen, which will probably
> > > occupy the a lot of microscopy time on the institute microscope.
> Because
> > I
> > > don't want to cause inconvenience for my coworkers and for reasons of
> > > imaging independence I am looking for a used wide field fluorescence
> > > microscope. A standard wide field system like the Axioskop2 series of
> > Zeiss
> > > as upright or even inverted design is sufficient for my purpose. I have
> > > some funding which I could use for purchasing and adapting it for my
> > needs.
> > >
> > > Has some one a good idea where I could find a used wide field system? I
> > am
> > > also happy about any other suggestion regarding sources of used
> > microscope
> > > equipment.
> > >
> > > Thanks a lot
> > >
> > > Martin
> > >
> > > -------------------------------------------------------------------
> > > Dr. Martin Schattat
> > > Martin-Luther-Universität Halle-Wittenberg
> > > Institutsbereich Pflanzenphysiologie
> > > Weinbergweg 10, 06120 Halle (Saale), Germany
> > >
> > > Tel.:     +49 345 5526286
> > > Email: [hidden email]
> > >
> >
>
Rosemary.White Rosemary.White
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

In reply to this post by Rosemary.White
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Looks like they save quite a bit of space, but are they cheaper? Easier to use? Give comparable results or better?
Might take a bit of getting used to, I guess.

cheers,
Rosemary
________________________________________
From: Cammer, Michael [[hidden email]]
Sent: Tuesday, 15 January 2013 8:36 a.m.
To: White, Rosemary (PI, Black Mountain)
Subject: RE: looking for used fluorescence microscope

Rosemary,
Two years ago we had a demonstration in our lab.  It looks nifty, but I'm not sure what the benefits are.
Regards,
Michael




-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Monday, January 14, 2013 4:28 PM
To: [hidden email]
Subject: Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

That looks really cool! Has anyone got one in their lab or at least tried one with their favourite tissue?

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Craig Brideau [[hidden email]]
Sent: Tuesday, 15 January 2013 6:40 a.m.
To: [hidden email]
Subject: Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You can also get kit-based solutions from a lot of vendors.  Scientifica and ThorLabs have some really nice little widefield systems at competitive prices.  Scientifica has this tiny little modular hexagonal stack that doesn't look like a microscope, but does a heck of a lot of widefield imaging techniques.  If you can forgo eyepieces and live with seeing everything on a monitor it might be an interesting (and cost effective) way to go.

http://www.scientifica.uk.com/products/imic-digital-imaging-platform

Check it out, even if just to see the pictures.  It looks... different.

Craig


On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Martin,
>
> I would suggest getting in touch with as many microscope sales reps
> and dealership owners as you can. Some dealers will buy up used
> microscopes when companies close or labs upgrade, and then resell them
> at a fraction of the new price. In my experience this is a word of
> mouth type business and not one that is easily found on the internet.
> The one person who I know does this is based in Utah, USA and I have
> lost contact with him so I doubt that he will be much help.
>
> Good Luck,
>
> Chris Tully
> Microscopy and Image Analysis Expert
> [hidden email]
> 240-475-9753 (c)
>
> [image: View my profile on LinkedIn]<
> http://www.linkedin.com/in/christully/>
>
>
> On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear List members,
> >
> > a few months ago I started my own plant cell biology lab in Halle
> (Saale),
> > Germany. I intend to do a microscopy based screen, which will
> > probably occupy the a lot of microscopy time on the institute
> > microscope. Because
> I
> > don't want to cause inconvenience for my coworkers and for reasons
> > of imaging independence I am looking for a used wide field
> > fluorescence microscope. A standard wide field system like the
> > Axioskop2 series of
> Zeiss
> > as upright or even inverted design is sufficient for my purpose. I
> > have some funding which I could use for purchasing and adapting it
> > for my
> needs.
> >
> > Has some one a good idea where I could find a used wide field
> > system? I
> am
> > also happy about any other suggestion regarding sources of used
> microscope
> > equipment.
> >
> > Thanks a lot
> >
> > Martin
> >
> > -------------------------------------------------------------------
> > Dr. Martin Schattat
> > Martin-Luther-Universität Halle-Wittenberg Institutsbereich
> > Pflanzenphysiologie Weinbergweg 10, 06120 Halle (Saale), Germany
> >
> > Tel.:     +49 345 5526286
> > Email: [hidden email]
> >
>
Barbara Foster Barbara Foster
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi, all

I've known the iMic for a number of years
now.  It is made by Till-Photonics and recently became part of the FEI family.

In answer to your questions, Rosemary:
iMIC is a highly engineered system and the
footprint is not quite as small as you might
imagine. Essentially it is a mult-layer optical
bench, offering flexibility that we haven't seen
since Zeiss discontinued the Axiomat decades
ago.  It is designed for advanced labs, for
multi-user systems where high stability and
exotic set-ups are required. The typical filter
cubes are "decoupled" in most of the systems I
have seen, so that you can program exactly which
exciter, dichroic, and barrier best fit a
specific experiment. Like an advanced car in
which you can set the steering wheel and seat
positions, iMIC can have multiple options
programmed for each user.  Just push the button
and the right filter sets, objectives, and
detectors move into position. Also, it offers
multiple "output" ports for cameras, microspectrophotometers, etc.

When I saw it last year in the FEI booth at
Pittcon, it had been installed on an amazing
moving stage in a system called CorrSight.  The
approach was novel: moving the microscope under
the sample, providing an elegant solution for
correlative microscopy.  FEI had paid close
attention to workflow and realized that it wasn't
just the problem of going from a light microscope
to the electron microscope but also to monitor
dynamic processes and being able to fix the cells at the appropriate instant.

So, for those of you looking for an elegant, high
end solution, iMIC is a good answer.  For those
of you involved in more routine analyses with
more conventional budgets, check out the usual
suspects for more standard configurations.

Hope this was helpful!

CAVEAT: I have no financial interest... this is
just a  lovely, unique microscope.

Best regards,
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education*
www.MicroscopyEducation.com

*A subsidiary of The Microscopy & Imaging Place, Inc.
7101 Royal Glen Trail, Suite A
McKinney, TX 75070
P: 972-924-5310
F: 214-592-0277

MME is currently scheduling courses for the Fall
2013. Call us today for a free training evaluation.



At 11:22 PM 1/13/2013, [hidden email] wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Looks like they save quite a bit of space, but
>are they cheaper? Easier to use? Give comparable results or better?
>Might take a bit of getting used to, I guess.
>
>cheers,
>Rosemary
>________________________________________
>From: Cammer, Michael [[hidden email]]
>Sent: Tuesday, 15 January 2013 8:36 a.m.
>To: White, Rosemary (PI, Black Mountain)
>Subject: RE: looking for used fluorescence microscope
>
>Rosemary,
>Two years ago we had a demonstration in our
>lab.  It looks nifty, but I'm not sure what the benefits are.
>Regards,
>Michael
>
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List
>[mailto:[hidden email]] On Behalf Of [hidden email]
>Sent: Monday, January 14, 2013 4:28 PM
>To: [hidden email]
>Subject: Re: looking for used fluorescence microscope
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>That looks really cool! Has anyone got one in
>their lab or at least tried one with their favourite tissue?
>
>Rosemary White
>CSIRO Plant Industry
>GPO Box 1600
>Canberra, ACT 2601
>Australia
>
>T 61 2 6246 5475
>F 61 2 6246 5334
>________________________________________
>From: Confocal Microscopy List
>[[hidden email]] On Behalf Of
>Craig Brideau [[hidden email]]
>Sent: Tuesday, 15 January 2013 6:40 a.m.
>To: [hidden email]
>Subject: Re: looking for used fluorescence microscope
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>You can also get kit-based solutions from a lot
>of vendors.  Scientifica and ThorLabs have some
>really nice little widefield systems at
>competitive prices.  Scientifica has this tiny
>little modular hexagonal stack that doesn't look
>like a microscope, but does a heck of a lot of
>widefield imaging techniques.  If you can forgo
>eyepieces and live with seeing everything on a
>monitor it might be an interesting (and cost effective) way to go.
>
>http://www.scientifica.uk.com/products/imic-digital-imaging-platform
>
>Check it out, even if just to see the pictures.  It looks... different.
>
>Craig
>
>
>On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Martin,
> >
> > I would suggest getting in touch with as many microscope sales reps
> > and dealership owners as you can. Some dealers will buy up used
> > microscopes when companies close or labs upgrade, and then resell them
> > at a fraction of the new price. In my experience this is a word of
> > mouth type business and not one that is easily found on the internet.
> > The one person who I know does this is based in Utah, USA and I have
> > lost contact with him so I doubt that he will be much help.
> >
> > Good Luck,
> >
> > Chris Tully
> > Microscopy and Image Analysis Expert
> > [hidden email]
> > 240-475-9753 (c)
> >
> > [image: View my profile on LinkedIn]<
> > http://www.linkedin.com/in/christully/>
> >
> >
> > On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat <
> > [hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Dear List members,
> > >
> > > a few months ago I started my own plant cell biology lab in Halle
> > (Saale),
> > > Germany. I intend to do a microscopy based screen, which will
> > > probably occupy the a lot of microscopy time on the institute
> > > microscope. Because
> > I
> > > don't want to cause inconvenience for my coworkers and for reasons
> > > of imaging independence I am looking for a used wide field
> > > fluorescence microscope. A standard wide field system like the
> > > Axioskop2 series of
> > Zeiss
> > > as upright or even inverted design is sufficient for my purpose. I
> > > have some funding which I could use for purchasing and adapting it
> > > for my
> > needs.
> > >
> > > Has some one a good idea where I could find a used wide field
> > > system? I
> > am
> > > also happy about any other suggestion regarding sources of used
> > microscope
> > > equipment.
> > >
> > > Thanks a lot
> > >
> > > Martin
> > >
> > > -------------------------------------------------------------------
> > > Dr. Martin Schattat
> > > Martin-Luther-Universität Halle-Wittenberg Institutsbereich
> > > Pflanzenphysiologie Weinbergweg 10, 06120 Halle (Saale), Germany
> > >
> > > Tel.:     +49 345 5526286
> > > Email: [hidden email]
> > >
> >
George McNamara George McNamara
Reply | Threaded
Open this post in threaded view
|

Re: looking for used fluorescence microscope .. consider the RAMM system 21118211

In reply to this post by Rosemary.White
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Martin,

Consider the "RAMM system"

http://www.asiimaging.com/products/video-microscopes-test-stands-complete-system-solutions/rapid-automated-modular-microscope-ramm-system/

I saw this in Hari Shroff's lab on his MSIM2 rig - Hari and Andrew York
used it for their MSIM, which they've now improved to MSIM2 acquiring 8x
confocal resolution optical sections at 25 fps. MSIM2 is a big speed
improvement over the MSIM that Andrew York, Hari and colleagues
published in 2012 in Nature Methods -
http://www.nature.com/nmeth/journal/v9/n7/full/nmeth.2025.html   and
http://code.google.com/p/msim/

I was at a "single cells, single molecules" meeting at U New Mexico last
week (  
http://stmc.health.unm.edu/single-cell-and-single-molecule-biology/index.html 
). Ginni Grover of Rafael Piestun's group presented their work on a much
simpler (less expensive) double helix PSF single molecule localization
system. See
http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-20-24-26681
for the publication.
By the way, W.E. Moerner had the best pronunciation of an acronym: SMACM
being "smack 'em" (much better than saying "Single-Molecule Active
Control Microscopies"). See PubMed 22582796 and/or 22577822 for reviews.
Also with resepct to the UNM meeting, Tom Jovin mentioned in his talk
that "iPAM" (intelligent PAM) is (will?) running at ~2000 fps. See
Pubmed 21118211 for most recent PAM paper, oe search for jovin
programmable array.

George
p.s. RAMM is also the stand for ASI's iSPIM selective illumination plane
microscopy unit.
http://www.asiimaging.com/products/light-sheet-microscopy/inverted-selective-plane-illumination-microscopy-ispim/
With respect to SPIM, colleagues here at UM are getting nice results of
YFP neuron expressing mouse brains, optically cleared, and imaged on the
LaVision Biotec Ultramicroscope SPIM system.
Moerner mentioned that he likes 2 um light sheet for his lab's DH-PSF SMACM.


On 1/14/2013 4:48 PM, [hidden email] wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Looks like they save quite a bit of space, but are they cheaper? Easier to use? Give comparable results or better?
> Might take a bit of getting used to, I guess.
>
> cheers,
> Rosemary
> ________________________________________
> From: Cammer, Michael [[hidden email]]
> Sent: Tuesday, 15 January 2013 8:36 a.m.
> To: White, Rosemary (PI, Black Mountain)
> Subject: RE: looking for used fluorescence microscope
>
> Rosemary,
> Two years ago we had a demonstration in our lab.  It looks nifty, but I'm not sure what the benefits are.
> Regards,
> Michael
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
> Sent: Monday, January 14, 2013 4:28 PM
> To: [hidden email]
> Subject: Re: looking for used fluorescence microscope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> That looks really cool! Has anyone got one in their lab or at least tried one with their favourite tissue?
>
> Rosemary White
> CSIRO Plant Industry
> GPO Box 1600
> Canberra, ACT 2601
> Australia
>
> T 61 2 6246 5475
> F 61 2 6246 5334
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] On Behalf Of Craig Brideau [[hidden email]]
> Sent: Tuesday, 15 January 2013 6:40 a.m.
> To: [hidden email]
> Subject: Re: looking for used fluorescence microscope
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> You can also get kit-based solutions from a lot of vendors.  Scientifica and ThorLabs have some really nice little widefield systems at competitive prices.  Scientifica has this tiny little modular hexagonal stack that doesn't look like a microscope, but does a heck of a lot of widefield imaging techniques.  If you can forgo eyepieces and live with seeing everything on a monitor it might be an interesting (and cost effective) way to go.
>
> http://www.scientifica.uk.com/products/imic-digital-imaging-platform
>
> Check it out, even if just to see the pictures.  It looks... different.
>
> Craig
>
>
> On Sat, Jan 12, 2013 at 10:43 AM, Chris Tully<[hidden email]>  wrote:
>
>    
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Martin,
>>
>> I would suggest getting in touch with as many microscope sales reps
>> and dealership owners as you can. Some dealers will buy up used
>> microscopes when companies close or labs upgrade, and then resell them
>> at a fraction of the new price. In my experience this is a word of
>> mouth type business and not one that is easily found on the internet.
>> The one person who I know does this is based in Utah, USA and I have
>> lost contact with him so I doubt that he will be much help.
>>
>> Good Luck,
>>
>> Chris Tully
>> Microscopy and Image Analysis Expert
>> [hidden email]
>> 240-475-9753 (c)
>>
>> [image: View my profile on LinkedIn]<
>> http://www.linkedin.com/in/christully/>
>>
>>
>> On Sat, Jan 12, 2013 at 12:19 PM, Martin Schattat<
>> [hidden email]>  wrote:
>>
>>      
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear List members,
>>>
>>> a few months ago I started my own plant cell biology lab in Halle
>>>        
>> (Saale),
>>      
>>> Germany. I intend to do a microscopy based screen, which will
>>> probably occupy the a lot of microscopy time on the institute
>>> microscope. Because
>>>        
>> I
>>      
>>> don't want to cause inconvenience for my coworkers and for reasons
>>> of imaging independence I am looking for a used wide field
>>> fluorescence microscope. A standard wide field system like the
>>> Axioskop2 series of
>>>        
>> Zeiss
>>      
>>> as upright or even inverted design is sufficient for my purpose. I
>>> have some funding which I could use for purchasing and adapting it
>>> for my
>>>        
>> needs.
>>      
>>> Has some one a good idea where I could find a used wide field
>>> system? I
>>>        
>> am
>>      
>>> also happy about any other suggestion regarding sources of used
>>>        
>> microscope
>>      
>>> equipment.
>>>
>>> Thanks a lot
>>>
>>> Martin
>>>
>>> -------------------------------------------------------------------
>>> Dr. Martin Schattat
>>> Martin-Luther-Universität Halle-Wittenberg Institutsbereich
>>> Pflanzenphysiologie Weinbergweg 10, 06120 Halle (Saale), Germany
>>>
>>> Tel.:     +49 345 5526286
>>> Email: [hidden email]
>>>
>>>        
>>      
>