luminescence

[Paul Herzmark]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal One of my users wants to look at low levels of translation using luciferase on the microscope. Has anyone imaged bioluminescence in transiently transfected cells before? It would be helpful if someone has any information on what kind of intracellular substrates to use.

I have a Coolsnap HQ CCD camera cooled
my brightest lens is a 40X 1.3 NA

Any suggestions, experience?


--
Paul Herzmark
Specialist
[hidden email]

Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA 94720-3200
[phone]
[phone] fax

[Martin Köhler]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Leica SP2 confocal users,

I wonder if anyone out there tried to fit a Leica HCX APO L 20x/1.00W lens on the Leica DM LFSA microscope stand?

/ Martin


-------------------------------------------------------------
 Martin Köhler
 The Rolf Luft Research Center for Diabetes and Endocrinology
 Karolinska Institutet
 Karolinska University Hospital Solna L1
 SE-171 76  Stockholm, Sweden

 E-mail:  [hidden email]
 Phone:   [phone]
 Fax:     [phone]
-------------------------------------------------------------

[Chris Tully]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Martin,

Although I no longer work with Leica, based on my great memory and the data you have listed, the lens you describe should physically fit the scope in question with no problem.  As far as I can recall, all HCX lenses are compatible with all DM series scopes, with possible exceptions of darkfield nose pieces and working distances that make it awkward (but no impossible) to put a lens designed for inverted scopes on an upright scope.  HCX is the designation for Leica's latest series of lenses.

Chris Tully

On Mon, Apr 14, 2008 at 2:18 PM, Martin Kohler <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Leica SP2 confocal users,

I wonder if anyone out there tried to fit a Leica HCX APO L 20x/1.00W lens on the Leica DM LFSA microscope stand?

/ Martin


-------------------------------------------------------------
 Martin Köhler
 The Rolf Luft Research Center for Diabetes and Endocrinology
 Karolinska Institutet
 Karolinska University Hospital Solna L1
 SE-171 76  Stockholm, Sweden

 E-mail:  [hidden email]
 Phone:   [phone]
 Fax:     [phone]
-------------------------------------------------------------

--
Chris Tully
Microscopy and Image Analysis Expert
[hidden email]
240-888-1021
http://www.linkedin.com/in/christully

[Armstrong, Brian]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Martin, Chris, I think that the problem will be that this lens will have a long focal distance such that you may need to alter your fixed stage configuration. Perhaps your local Leica rep could bring one so that you could try it. I would like to hear the results.

Cheers,

 

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Chris Tully
Sent: Monday, April 14, 2008 4:12 PM
To: [hidden email]
Subject: Re: Leica 20x question

 

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Martin,

Although I no longer work with Leica, based on my great memory and the data you have listed, the lens you describe should physically fit the scope in question with no problem.  As far as I can recall, all HCX lenses are compatible with all DM series scopes, with possible exceptions of darkfield nose pieces and working distances that make it awkward (but no impossible) to put a lens designed for inverted scopes on an upright scope.  HCX is the designation for Leica's latest series of lenses.

Chris Tully

On Mon, Apr 14, 2008 at 2:18 PM, Martin Kohler <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Leica SP2 confocal users,

I wonder if anyone out there tried to fit a Leica HCX APO L 20x/1.00W lens on the Leica DM LFSA microscope stand?

/ Martin


-------------------------------------------------------------
 Martin Köhler
 The Rolf Luft Research Center for Diabetes and Endocrinology
 Karolinska Institutet
 Karolinska University Hospital Solna L1
 SE-171 76  Stockholm, Sweden

 E-mail:  [hidden email]
 Phone:   [phone]
 Fax:     [phone]
-------------------------------------------------------------

--
Chris Tully
Microscopy and Image Analysis Expert
[hidden email]
240-888-1021
http://www.linkedin.com/in/christully

---------------------------------------------------------------------
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[Guy Cox]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

That lens is HUGE!  The focussing nosepiece on a fixed stage might

have trouble with the weight.  As Brian says, you should try before

you buy. 

 

                                                                                      Guy

 

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
______________________________________________
     http://www.guycox.net

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Armstrong, Brian
Sent: Tuesday, 15 April 2008 9:49 AM
To: [hidden email]
Subject: Re: Leica 20x question

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Martin, Chris, I think that the problem will be that this lens will have a long focal distance such that you may need to alter your fixed stage configuration. Perhaps your local Leica rep could bring one so that you could try it. I would like to hear the results.

Cheers,

 

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Chris Tully
Sent: Monday, April 14, 2008 4:12 PM
To: [hidden email]
Subject: Re: Leica 20x question

 

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Martin,

Although I no longer work with Leica, based on my great memory and the data you have listed, the lens you describe should physically fit the scope in question with no problem.  As far as I can recall, all HCX lenses are compatible with all DM series scopes, with possible exceptions of darkfield nose pieces and working distances that make it awkward (but no impossible) to put a lens designed for inverted scopes on an upright scope.  HCX is the designation for Leica's latest series of lenses.

Chris Tully

On Mon, Apr 14, 2008 at 2:18 PM, Martin Kohler <[hidden email]> wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Leica SP2 confocal users,

I wonder if anyone out there tried to fit a Leica HCX APO L 20x/1.00W lens on the Leica DM LFSA microscope stand?

/ Martin


-------------------------------------------------------------
 Martin Köhler
 The Rolf Luft Research Center for Diabetes and Endocrinology
 Karolinska Institutet
 Karolinska University Hospital Solna L1
 SE-171 76  Stockholm, Sweden

 E-mail:  [hidden email]
 Phone:   [phone]
 Fax:     [phone]
-------------------------------------------------------------

--
Chris Tully
Microscopy and Image Analysis Expert
[hidden email]
240-888-1021
http://www.linkedin.com/in/christully

---------------------------------------------------------------------

SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender.

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[Rosemary.White]

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

No, only on a DMR, and works great.  We have all the dipping lenses, they're
fantastic for looking at very 3D live things, like plant shoot apical
meristems.
good luck,
Rosemary


Rosemary White                    [hidden email]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     [phone]
Canberra, ACT 2601
Australia


On 15/4/08 4:18 AM, "Martin Kohler" <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Leica SP2 confocal users,
>
> I wonder if anyone out there tried to fit a Leica HCX APO L 20x/1.00W lens on
> the Leica DM LFSA microscope stand?
>
> / Martin
>
>
> -------------------------------------------------------------
>  Martin Köhler
>  The Rolf Luft Research Center for Diabetes and Endocrinology
>  Karolinska Institutet
>  Karolinska University Hospital Solna L1
>  SE-171 76  Stockholm, Sweden
>
>  E-mail:  [hidden email]
>  Phone:   [phone]
>  Fax:     [phone]
> -------------------------------------------------------------