microscope cooling
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Dear all
We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Sylvie Upgrading your CUBE&BOX temperature control system with an iceCUBE heating&cooling controller will allow you to do exactly that. That's a bit of a shameless plug, I know, so please contact me off-list if you want more information :-) Cheers, Beat At 13:54 20-09-11, you wrote: >Content-Transfer-Encoding: base64Dear all > >We have a spinning disk system with an incubator >and a heating unit which usually runs at 37ËC. > >One of our users needs the temperature to be >stable at 20ËC. Turning the heating unit off >unfortunately only brings the system to 25ËC so >we only need to cool it down by 5ËC. Turning on >the air condition in the room doesnt help >because we have several microscopes in that room >so the air doesnt circulate well enough. >Ideally we would want a cooling system to cool >down the air that goes to the input of our >heating unit so that the unit can still control the temperature. > >Does anyone have some experience with cooling a >microscope to 20ËC? Any recommendation is welcome! ⺠> >Med vänlig hälsning / Best regards > >Sylvie > >@@@@@@@@@@@@@@@@@@@@@@@@ >Sylvie Le Guyader >Live Cell Imaging Unit >Dept of Biosciences and Nutrition >Karolinska Institutet >Novum >14183 Huddinge >Sweden >office: +46 (0) 8 5248 1107 >LCI room: +46 (0) 8 5248 1172 >mobile: +46 (0) 73 733 5008 |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi That is going to be tricky. You could pump refrigerated water through the incubator in pipes to chill it down. I would be careful of condensation though. If the microscope/incubator is cooled significantly below ambient and the humidity is too high you could get water condensing on come important parts. Colin Dr Colin Rickman Life Science Interface Department of Chemistry (WP 2.03) School of Engineering and Physical Sciences Heriot-Watt University Edinburgh EH14 4AS Tel: +44 131 4514193 (Office) http://www.lifescienceinterface.hw.ac.uk http://www.eps.hw.ac.uk/departments/chemistry/cr.htm On 20 Sep 2011, at 12:54, Sylvie LeGuyader wrote: > Dear all > > We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > -- Heriot-Watt University is a Scottish charity registered under charity number SC000278. |
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Hi Colin
Yes but my hope is that since we are only cooling it down to 20˚C, that will not be a problem. Has anyone had that type of trouble? Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Colin Rickman > Sent: 20 September 2011 14:17 > To: [hidden email] > Subject: Re: microscope cooling > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi > > That is going to be tricky. You could pump refrigerated water through the incubator > in pipes to chill it down. I would be careful of condensation though. If the > microscope/incubator is cooled significantly below ambient and the humidity is too > high you could get water condensing on come important parts. > > Colin > > Dr Colin Rickman > Life Science Interface > Department of Chemistry (WP 2.03) > School of Engineering and Physical Sciences > Heriot-Watt University > Edinburgh > EH14 4AS > > Tel: +44 131 4514193 (Office) > > http://www.lifescienceinterface.hw.ac.uk > http://www.eps.hw.ac.uk/departments/chemistry/cr.htm > > On 20 Sep 2011, at 12:54, Sylvie LeGuyader wrote: > > > Dear all > > > > We have a spinning disk system with an incubator and a heating unit which > usually runs at 37˚C. > > > > One of our users needs the temperature to be stable at 20˚C. Turning the heating > unit off unfortunately only brings the system to 25˚C so we only need to cool it > down by 5˚C. Turning on the air condition in the room doesn’t help because we > have several microscopes in that room so the air doesn’t circulate well enough. > Ideally we would want a cooling system to cool down the air that goes to the input > of our heating unit so that the unit can still control the temperature. > > > > Does anyone have some experience with cooling a microscope to 20˚C? Any > recommendation is welcome! ☺ > > > > Med vänlig hälsning / Best regards > > > > Sylvie > > > > @@@@@@@@@@@@@@@@@@@@@@@@ > > Sylvie Le Guyader > > Live Cell Imaging Unit > > Dept of Biosciences and Nutrition > > Karolinska Institutet > > Novum > > 14183 Huddinge > > Sweden > > office: +46 (0) 8 5248 1107 > > LCI room: +46 (0) 8 5248 1172 > > mobile: +46 (0) 73 733 5008 > > > > > > -- > Heriot-Watt University is a Scottish charity > registered under charity number SC000278. |
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In reply to this post by Sylvie Le Guyader-2
I'm a bit out of my depth here but I do know that we have a system that will cool as well as heat. I'm copying this to Ellie Kable and I hope she will post some details.
Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sylvie LeGuyader Sent: Tuesday, 20 September 2011 9:54 PM To: [hidden email] Subject: microscope cooling Dear all We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1410 / Virus Database: 1520/3907 - Release Date: 09/19/11 |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We regularly go to 16C with a ThermoNeslab constant temperature recirculating bath that is capable of 2C to 100C. The fluid is pumped through a PECON stage insert on a Zeiss LSM 710 inverted system. http :// www . pecon .biz/?page_id=1278 We have not had a problem with condensation. We more regularly use the system at 37C. Louie ----- Original Message ----- From: "Sylvie LeGuyader " <Sylvie. LeGuyader @KI.SE> To: CONFOCALMICROSCOPY @LISTS. UMN . EDU Sent: Tuesday, September 20, 2011 7:54:19 AM Subject: microscope cooling Dear all We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn ’t help because we have several microscopes in that room so the air doesn ’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 -- Louis Kerr lkerr @ mbl . edu Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone) VISIT OUR WEB SITES: www . mbl . edu www .courses. mbl . edu |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have a OKlab system. I can try it by tomorrow how stable it is... Best, Tineke Vendrig TU Delft, Applied sciences Kavli institute Lorentzweg 1 2628 CJ Delft The Netherlands phone: +31 15 2789299 fax: +31 15 2781202 2011/9/20 Sylvie LeGuyader <[hidden email]> > Dear all > > We have a spinning disk system with an incubator and a heating unit which > usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the > heating unit off unfortunately only brings the system to 25˚C so we only > need to cool it down by 5˚C. Turning on the air condition in the room > doesn’t help because we have several microscopes in that room so the air > doesn’t circulate well enough. Ideally we would want a cooling system to > cool down the air that goes to the input of our heating unit so that the > unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any > recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > > |
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In reply to this post by Louis Kerr
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If you know the relative humidity, you can look up the dew point... Cheers On 20/09/2011, at 1:31 PM, Louis Kerr wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We regularly go to 16C with a ThermoNeslab constant temperature recirculating bath that is capable of 2C to 100C. The fluid is pumped through a PECON stage insert on a Zeiss LSM 710 inverted system. > > http :// www . pecon .biz/?page_id=1278 > > We have not had a problem with condensation. We more regularly use the system at 37C. > > Louie > > ----- Original Message ----- > From: "Sylvie LeGuyader " <Sylvie. LeGuyader @KI.SE> > To: CONFOCALMICROSCOPY @LISTS. UMN . EDU > Sent: Tuesday, September 20, 2011 7:54:19 AM > Subject: microscope cooling > > Dear all > > We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn ’t help because we have several microscopes in that room so the air doesn ’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > > > > -- > > > Louis Kerr > lkerr @ mbl . edu > > Research and Education Support Coordinator > Marine Biological Laboratory > 7 MBL Street > Woods Hole, MA 02543 > 508-289-7273 > 508-540-6902 (FAX) > 508-292-0289 (Cell phone) > > VISIT OUR WEB SITES: > www . mbl . edu > www .courses. mbl . edu |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Sylvie, We have a cooling unit installed on one of our DeltaVisions and regularly use it at 20 degrees with no problems. Unfortunately though I think it's the only one of its kind - it came with our "Weatherstation" environmental chamber supplied by API, but they have now discontinued the cooling part so I don't think you would be able to buy the same model. However, I just wanted to reassure you that it is perfectly possible to keep the focus stable when cooling with the large type chamber, as well as with smaller systems such as the Oko lab stage-top heater/cooler (we also have one of those). Another possibility would be to use a Warner Instruments peltier cooled device. We have one that fits exactly into a Prior piezo stage (i.e. it's the same size as the regular piezo insert) and this also works nicely - we just stick this inside our larger environmental chamber (obviously with the heat switched off) on our spinning disk when we need to cool instead of heat. You can tell I have lots of users who work on yeast and worms... Good luck! All the best, Alison On 9/20/2011 7:54 AM, Sylvie LeGuyader wrote: > Dear all > > We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 |
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In reply to this post by Sylvie Le Guyader-2
Years ago (almost 20) we had a stage that we could pump water through to keep at temperature. It was a metal stage insert (for a Nikon Diaphot) that had channels for water inside it and fittings to put hoses on. We had a cold water bath and a warm water bath. We used cold water to hold material in the Golgi and then we'd up the temperature to 37 to release. And we'd have to rack the focus like crazy during imaging, but an autofocus system like Nikon's PFS should be able to compensate.
There must be something like this still on the market (WPI?) or you could probably make one easily. Later, for a different user, we did a more dirtball approach. We put small bags of ice on a heater stage insert that was off. At the correct time we carefully lifted them off and turned on the heater. Inelegant and we didn't get smooth movies, but it got the job done for what we needed to see. ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sylvie LeGuyader Sent: Tuesday, September 20, 2011 7:54 AM To: [hidden email] Subject: microscope cooling Dear all We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Sylvie The situation you describe alerts me to be cautious of several possibilities. 1. Spinning Disk Confocal = narrow depth of field concerns. You need to be sure that the temperature change does not induce thermal drift. 2. Condensation at a temperature that close to ambient will depend on your room humidity. In that there is air conditioning in the room I would expect the humidity to be low. 3. What lens are you using for your imaging? Is it fluid coupled or dry? If fluid coupled such as oil, glycerin or water the point of contact of the fluid coupling will have the most dominant effect on specimen temperature. Therefore, it may be necessary to control the lens temperature. 4. Is your entire microscope boxed or are you intending to use a stage top micro-incubator? 5. What is the specimen and what structure is it placed in, do the microscopy. 6. Does the specimen need any special external support. Perfusion, gas, stimulation, etc. 7. Do you need to be able to change temperature quickly during the experiment or are you looking for consistency? 8. See below! I am reasonably sure that we may have a solution for you. Please goto our web site www.bioptechs.com and fill out the User Profile Questionnaire available from a link on the home page and send it in to me. This will give me a better idea of your requirements. Bioptechs specializes in micro-environmental control for time-lapse imaging. We address the details of setting up live-cell experiments with great care to properly identify the most appropriate equipment. If we do not have a solution, that is unusual but we can and will recommend other brands of systems if ours is not optimal for your needs. Dan On Sep 20, 2011, at 7:54 AM, Sylvie LeGuyader wrote: Dear all We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ Med vänlig hälsning / Best regards Sylvie @@@@@@@@@@@@@@@@@@@@@@@@ Sylvie Le Guyader Live Cell Imaging Unit Dept of Biosciences and Nutrition Karolinska Institutet Novum 14183 Huddinge Sweden office: +46 (0) 8 5248 1107 LCI room: +46 (0) 8 5248 1172 mobile: +46 (0) 73 733 5008 Dan Focht Bioptechs, Inc. 3560 Beck Rd. Butler, PA 16002 www.bioptechs.com P: (724)282-7145 F: (724)282-0745 [hidden email] |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Good evening, many years ago, I worked in a nuclear physics laboratory, where we were operating a Coherent CR18 graphite ion laser and a CR699-21 ring dye laser, which always was open. In order to keep the air dust free and the temperature stable, we had a laminar flow system covering the entire laser bench. The "walls" of that system was a curtain of thick and soft vertical plastic ribbons. This system worked pretty well. There are a number of commercial producers of these hoods, which can be fixed in the ceiling and also include ligthing. Unfortunately, I do not have the slightest idea what these devices cost in our days (the last time I was active in this lab was in the late 1980s). A good source of information in Stockholm might be a department of laser physics or laser spectroscopy at SU or KTH or MSI. Best wishes, Johannes > Dear all > > We have a spinning disk system with an incubator and a heating unit which > usually runs at 37?C. > > One of our users needs the temperature to be stable at 20?C. Turning the > heating unit off unfortunately only brings the system to 25?C so we only > need to cool it down by 5?C. Turning on the air condition in the room > doesn?t help because we have several microscopes in that room so the air > doesn?t circulate well enough. Ideally we would want a cooling system to > cool down the air that goes to the input of our heating unit so that the > unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20?C? Any > recommendation is welcome! ? > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > > -- P. Johannes Helm Voice: (+47) 228 51159 (office) Fax: (+47) 228 51499 (office) |
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Live Cell Instrument makes exactly this part. It is a stage insert that has water circulator input/output ports. It is very cost effective and works great. It is called a water circulation cooling plate (CP-R-XX) and is available for a variety of stages. They also have a water circulation chamber called the Chamlide WC (WC-RXX). It is available for various sized coverslips. LCI products are available from Quorum Technologies Inc. in North America and Micro System AB in Sweden. Cheers Ryan Quorum Technologies Inc. Installation/Support Tech LCI Product Manager 519-400-0900 Sent from my iPhone On 2011-09-20, at 10:25 AM, "Cammer, Michael" <[hidden email]> wrote: > Years ago (almost 20) we had a stage that we could pump water through to keep at temperature. It was a metal stage insert (for a Nikon Diaphot) that had channels for water inside it and fittings to put hoses on. We had a cold water bath and a warm water bath. We used cold water to hold material in the Golgi and then we'd up the temperature to 37 to release. And we'd have to rack the focus like crazy during imaging, but an autofocus system like Nikon's PFS should be able to compensate. > There must be something like this still on the market (WPI?) or you could probably make one easily. > > Later, for a different user, we did a more dirtball approach. We put small bags of ice on a heater stage insert that was off. At the correct time we carefully lifted them off and turned on the heater. Inelegant and we didn't get smooth movies, but it got the job done for what we needed to see. > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sylvie LeGuyader > Sent: Tuesday, September 20, 2011 7:54 AM > To: [hidden email] > Subject: microscope cooling > > Dear all > > We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. > ================================= |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We have a microscope fitted with a wrap-around incubator (entire upper body of scope is inside incubator), and it also does get warm because it traps the heat of the stage and microscope). We purchased a small portable air conditioner. When we need less than 25oC, we divert the air intake of the heater/fan unit to collect cool air from the air conditioner (and just leave the fan of the heater unit running, with heating off). (we just connected a "hood" to the air intake tubes and mounted it on top of the air conditioner vents. The air conditioner was about $ 300. It does generate some noise and vibration, and probably changes the humidity levels of the room, but none of those have been a problem so far. I would say this system is effective for cooling the sample down to about 20-22 oC. Precise control of the temperature inside the incubator may be a bit tricky though. The hot air of the air conditioner is piped out of the room through the active vents used to dissipate the heat from our lasers. For further cooling, we have run cold water from a Lauda chilling recirculator through copper tubing wrapped around the objective. A more elegant (and precise) alternative as many have suggested would be to purchase a cooling stage top incubator, either Peltier, or water-cooled. Such stage-top heating/cooling incubators are available from several vendors, and would allow to cool samples to temperatures below ambient. However, such systems run in the $ 15K. For occasional use and non-demanding applications though, we found the air conditioner to be adequate. -- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA http://www.fhcrc.org/ On Sep 20, 2011, at 4:54 AM, Sylvie LeGuyader wrote: > Dear all > > We have a spinning disk system with an incubator and a heating unit which usually runs at 37˚C. > > One of our users needs the temperature to be stable at 20˚C. Turning the heating unit off unfortunately only brings the system to 25˚C so we only need to cool it down by 5˚C. Turning on the air condition in the room doesn’t help because we have several microscopes in that room so the air doesn’t circulate well enough. Ideally we would want a cooling system to cool down the air that goes to the input of our heating unit so that the unit can still control the temperature. > > Does anyone have some experience with cooling a microscope to 20˚C? Any recommendation is welcome! ☺ > > Med vänlig hälsning / Best regards > > Sylvie > > @@@@@@@@@@@@@@@@@@@@@@@@ > Sylvie Le Guyader > Live Cell Imaging Unit > Dept of Biosciences and Nutrition > Karolinska Institutet > Novum > 14183 Huddinge > Sweden > office: +46 (0) 8 5248 1107 > LCI room: +46 (0) 8 5248 1172 > mobile: +46 (0) 73 733 5008 > |
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In reply to this post by Sylvie Le Guyader-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi, I did several years ago, a cooling system on a box to cool it to 17 ° Celsius. For this I used a cooler effect controlled by a home made Pelletier controller . It's really easy to do. As a cooler car. I can send you some picture of the system. Regards Pascal Weber CNRS-UMR6020 Faculty of Medecine 27 boulevard Jean Moulin 13385 Marseille cedex 05 France mail: [hidden email] |
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