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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Colleagues, We will do FISH with sperm cells from HIV + patients. Before I do FISH, I will check my slides under microscope. Would anybody tell me how to disinfect microscopes? Any other tips as well? Thanks. Yuwei Zhang, MD, PhD Department of Environmental and Occupational Health The George Washington University 2300 I Street NW Room 112 Washington, DC 20052 Tel: 202-994-6023 (Office), 202-994-5327 (Lab) Fax: 202-994-0011(Office), 206-350-7510 (Personal) Email: [hidden email] http://sphhs.gwu.edu/departments/environmental-and-occupational-health/dep artmental-research |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Yuwei Zhang, When this situation ever comes up, I recommend doing everything you can possibly do to avoid contaminating the microscope in the first place. By this I would consider the following: 1. Seal your FISH labelled samples on the slide under a coverslip (perhaps using nail polish), 2. Decontaminate all surfaces of the slides (after the jail polish has set). Use whatever is process is recommended for ensuring adequate decontamination (but not UV exposure) 3. Prevent contamination of the slide in the first place by handling it correctly (ie make sure material is not transferred onto other parts of the slide by transfer from your gloves etc). As a general comment, you do not want to be in a position where your microscope might need to be decontaminated. Cheers Paul Assoc. Prof. Paul Rigby Centre for Microscopy, Characterisation & Analysis (M510) The University of Western Australia 35 Stirling Highway Crawley WA 6007 Australia -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zhang, Yuwei Sent: Wednesday, 5 February 2014 11:13 PM To: [hidden email] Subject: microscope disinfection ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Colleagues, We will do FISH with sperm cells from HIV + patients. Before I do FISH, I will check my slides under microscope. Would anybody tell me how to disinfect microscopes? Any other tips as well? Thanks. Yuwei Zhang, MD, PhD Department of Environmental and Occupational Health The George Washington University 2300 I Street NW Room 112 Washington, DC 20052 Tel: 202-994-6023 (Office), 202-994-5327 (Lab) Fax: 202-994-0011(Office), 206-350-7510 (Personal) Email: [hidden email] http://sphhs.gwu.edu/departments/environmental-and-occupational-health/dep artmental-research |
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