more on resolution in reflection

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more on resolution in reflection

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Hello:

 

I made a few quick scans to compare image quality in two situations: regular wide field and wide-field illumination coupled with confocal detection. The latter looks like an odd way to do things, but I have some reflection applications in mind where it might be useful. So, it seems to me that this hybrid imaging mode doesn’t provide true sectioning (finite z-dimension of an image of a plane) but still improves resolution. Does anyone know of any relevant theory or experimental data? Many thanks in advance.

 

Michael Model, Ph.D.

Confocal Microscopy Core

Dpt. Biological Sciences

Kent State University

Kent, OH 44242

tel. 330-672-2874