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I need an acute protocol to mark isolated hepatocytes (with CFSE) obtained by
collagenase digestion. I transplant isolated heptocyte to partial hepatectomized
(3/4) ratÂ’s livers and I need to differentiate donor hepatocytes from recipient
ones. Isolated hepatocytes are obtained in Ringer solution. I proved with some
protocols in order to label the cells before transplant, but I failed. I obtained no
mark with confocal exploration. The marker is 5-(and-6)-Carboxyfluorescein
Diacetate, Succinimidyl Ester (5(6)-CFDA, SE; CFSE) - Mixed Isomers from
Invitrogen. I recently bought the mark. I transplant 150.000 hepatocytes / ml.
If anybody has an appropriate and acute method to stain the cells berfore
transplant (with CFSE) I should be glad to received the protocol so as not to lose
time, mark and rats. Thank you very much. Dra. Alejandra Quintana
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