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Hi Alby,
Thannks for publishing open access.
What about STED-like (RESOLFT) scanning of YtvA (or relatives) - as done
by Testa et al with DronPA-M159T?
http://www.ncbi.nlm.nih.gov/pubmed/22998868Testa I, Urban NT, Jakobs S, Eggeling C, Willig KI, Hell SW. Nanoscopy
of living brain slices with low light levels. Neuron. 2012 Sep
20;75(6):992-1000. doi: 10.1016/j.neuron.2012.07.028. PubMed PMID:
22998868.
Speaking of STED, has anyone used laser mode(s) similar to those
described by Zuchner et al to make doughnuts for STED and STED-like
scanning:
http://www.ncbi.nlm.nih.gov/pubmed?term=21591027Züchner T, Failla AV, Meixner AJ. Light microscopy with doughnut modes:
a concept to detect, characterize, and manipulate individual
nanoobjects. Angew Chem Int Ed Engl. 2011 May 27;50(23):5274-93. doi:
10.1002/anie.201005845. Epub 2011 May 17. Review. PubMed PMID: 21591027.
Fans of LOV (re: Alby's paper) may find of interest:
http://www.ncbi.nlm.nih.gov/pubmed?term=22439625Potzkei J, Kunze M, Drepper T, Gensch T, Jaeger KE, Büchs J. Real-time
determination of intracellular oxygen in bacteria using a genetically
encoded FRET-based biosensor. BMC Biol. 2012 Mar 22;10:28. PubMed PMID:
22439625; PubMed Central PMCID: PMC3364895.
thanks in advance,
George
On 10/9/2012 1:28 PM, Alberto Diaspro wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Dear friends
> maybe you are interested in such a paper: 1. Losi, A., Gärtner, W., Raffelberg, S., Cella Zanacchi, F., Bianchini, P., Diaspro, A., Mandalari, C., S. Abruzzetti, C. Viappiani (2013). A photochromic bacterial photoreceptor with potential for super-resolution microscopy. Photochemical& Photobiological Sciences.
http://xlink.rsc.org/?DOI=c2pp25254f (open access)
> All the best
> Alby
>
Locked
