Hi Michelle,
I haven't used this, but have you thought of trying to tag eosinophil peroxidase?
http://www.ncbi.nlm.nih.gov/pubmed/11978925?dopt=AbstractPlus
I see there are a few antibodies commercially available. If I haven't previously used an antibody, I usually ask for a free sample unless the company can provide compelling images specific to my fixation/embedding/etc.
Best,
Nate
On Fri, Sep 25, 2009 at 1:15 PM, Carl Boswell
<[hidden email]> wrote:
Hi all,
Eosinophils get their name from their granules that
stain with eosin. Eosin also stains other cells and tissue material,
so it would not necessarily be cell-specific. It might be worth a try,
though, just to see if the differential staining properties to eosin were enough
to distinguish cell types.
C
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of
Arizona
520-954-7053
FAX 520-621-3709
----- Original Message -----
Sent: Friday, September 25, 2009 5:24
AM
Well, eosin itself is fluorescent. We get some remarkable
flluorescence images of H&E stained intestine
sections.
Joel
On Fri, Sep 25, 2009 at 1:59 AM, Michelle Brownlee
<[hidden email]>
wrote:
Hello every one,
This is my first question for
some help.
I was wondering what would be the best way to stain
eosinophils for
confocal. We are planning on staining lungs +/- asthma in
mice. We would
like to see T cells (will use CD4) and eosinophils. The
sections are OCT
embedded.
Any and all answers
appreciated
Thanks
Michelle
--
Joel B. Sheffield, Ph.D
Department of
Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204
8839
e-mail: [hidden email]
URL:
http://astro.temple.edu/~jbs
--
Nathan O'Connor
Silver Laboratory, Physiology and Biophysics D-503
Weill Cornell Medical College
New York, NY 10065
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