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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list friends, How do I calculate the divergence of fluorescence from a diffraction-limited spot in an infinity focus system with increasing path length? Thanks for help, Roger Dr Roger Guy Phillips Centre for Advanced Microscopy, University of Sussex School of Life Sciences John Maynard Smith Building Falmer, Brighton & Hove BN1 9QG United Kingdom phone:44 (0)1273 877585 fax: 44 (0)1273 678433 email: [hidden email] room:2C9 (ext 7585)/lab 4C2 (ext 2734) |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Roger, Not sure about the question. To the best of my understanding (and I'm not a physicist), within the 'infinity space' the rays from a spot will not diverge but will remain parallel. They will diverge from the optic axis depending on the distance of the spot from the centre of the field. (That's why the 'infinity space' is actually very finite.) Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Roger Phillips Sent: Thursday, 24 January 2008 10:54 PM To: [hidden email] Subject: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list friends, How do I calculate the divergence of fluorescence from a diffraction-limited spot in an infinity focus system with increasing path length? Thanks for help, Roger Dr Roger Guy Phillips Centre for Advanced Microscopy, University of Sussex School of Life Sciences John Maynard Smith Building Falmer, Brighton & Hove BN1 9QG United Kingdom phone:44 (0)1273 877585 fax: 44 (0)1273 678433 email: [hidden email] room:2C9 (ext 7585)/lab 4C2 (ext 2734) No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.19.10/1240 - Release Date: 23/01/2008 5:47 PM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.19.10/1240 - Release Date: 23/01/2008 5:47 PM |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Yes, I think that is right, the finite field size limits the collimation angle to field size/focal length. Cheers Mark -----Original Message----- From: Confocal Microscopy List on behalf of Guy Cox Sent: Fri 1/25/2008 1:09 AM To: [hidden email] Subject: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Roger, Not sure about the question. To the best of my understanding (and I'm not a physicist), within the 'infinity space' the rays from a spot will not diverge but will remain parallel. They will diverge from the optic axis depending on the distance of the spot from the centre of the field. (That's why the 'infinity space' is actually very finite.) Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Roger Phillips Sent: Thursday, 24 January 2008 10:54 PM To: [hidden email] Subject: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list friends, How do I calculate the divergence of fluorescence from a diffraction-limited spot in an infinity focus system with increasing path length? Thanks for help, Roger Dr Roger Guy Phillips Centre for Advanced Microscopy, University of Sussex School of Life Sciences John Maynard Smith Building Falmer, Brighton & Hove BN1 9QG United Kingdom phone:44 (0)1273 877585 fax: 44 (0)1273 678433 email: [hidden email] room:2C9 (ext 7585)/lab 4C2 (ext 2734) No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.19.10/1240 - Release Date: 23/01/2008 5:47 PM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.19.10/1240 - Release Date: 23/01/2008 5:47 PM |
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