Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHello Tino,
One of my users is "gene-gunning" DiI on 100 µm thick brain sections to
stain neurons for further confocal imaging to count dendritic microspines.
As he is also interested in performing immunofluorescence after imaging the
spines, he is using CM DiI, wich is more resistant to permeabilization and
washings than normal DiI. It took him 3-4 attempts to find the correct
settings of the Gene Gun (mainly the thickness of the protective membrane),
but in the end is a reasonabily easy technique.
CM DiI:
https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&productDescription=7159
Hope this helps,
Xavi.
___________________________________
Xavier Sanjuan
Servei de Microscòpia Confocal
Departament de Ciències Experimentals i de la Salut
Universitat Pompeu Fabra
Parc de Recerca Biomèdica de Barcelona
Doctor Aiguader, 88 - Sala 309
08003 Barcelona - Spain
Tel.: + 34 93 316 08 64
Fax: + 34 93 316 09 01
E-mail:
[hidden email] <mailto:
[hidden email]>
Web:
http://www.upf.edu/cexs/sct <
http://www.upf.edu/cexs/sct>
-----Mensaje original-----
De: Confocal Microscopy List [mailto:
[hidden email]]En
nombre de Tino Jäger
Enviado el: dijous, 20 / març / 2008 14:43
Para:
[hidden email]
Asunto:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalIs there any immunohistochemical marker you could suggest to use for
staining neuronal cells in fixed tissue to follow up the surface of the
neurons up to the level of dendritic spines for further co-staining and
analysis by confocal microscopy?
Thank you for your help,
Tino
--
Dr. Tino Jäger
Department of Neurophysiology
Center for Brain Research
Medical University of Vienna
Spitalgasse 4, A-1090 Vienna
Tel: +43-1-4277-62847
Secr: +43-1-4277-62835
FAX: +43-1-4277-62865
http://www.meduniwien.ac.at
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