(no subject)

[manojshree]

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Hello folks,

I am looking for a dye for blood vessels labeling in the blue emission
range. Any suggestion. Cascade blue did not work well in our hand and i did
not find any dextean conjugates in blue color.

Thanks,
ManOj

[Dagmar Adeline Brüggemann]

Dear Manoj

Depending on the category of blood vessels you are interested in, most of them  contain elastin (but not capillaries) and this can be easily visualized by 2P -flourescence λex 700-750 (nm)- so you would not need any additional labeling. A more detailed classification could be performed by a combination of SHG for collagen and 2P fluorescence for collagen vs elastin as arteries and arterioles have mush thicker walls than venes and venules as well as  lymph vessels.

Dagmar



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Manoj Jaiswal
Sent: 5. august 2012 11:44
To: [hidden email]
Subject:

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Hello folks,

I am looking for a dye for blood vessels labeling in the blue emission range. Any suggestion. Cascade blue did not work well in our hand and i did not find any dextean conjugates in blue color.

Thanks,
ManOj

[Alberto Diaspro]

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Dear Manoj
please, have a check here:
http://onlinelibrary.wiley.com/doi/10.1002/jbio.200810060/abstract
ciao
Alby


Il giorno 06/ago/2012, alle ore 17:30, Dagmar Adeline Brüggemann <[hidden email]> ha scritto:

> Dear Manoj
>
> Depending on the category of blood vessels you are interested in, most of them  contain elastin (but not capillaries) and this can be easily visualized by 2P -flourescence λex 700-750 (nm)- so you would not need any additional labeling. A more detailed classification could be performed by a combination of SHG for collagen and 2P fluorescence for collagen vs elastin as arteries and arterioles have mush thicker walls than venes and venules as well as  lymph vessels.
>
> Dagmar
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Manoj Jaiswal
> Sent: 5. august 2012 11:44
> To: [hidden email]
> Subject:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello folks,
>
> I am looking for a dye for blood vessels labeling in the blue emission range. Any suggestion. Cascade blue did not work well in our hand and i did not find any dextean conjugates in blue color.
>
> Thanks,
> ManOj
>
>
>