nosepiece Olympus BX51WI two-photon mount

["José A. Feijó"]

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I'm trying to get a Bio-rad two-photon with and Olympus BX51WI optics
aup and running. I need a nosepiece for that, but the lenses I have are
all large barrel. Olympus sells a 6 position rotating one, but with the
old, thinner thread. And then they have a very expensive sliding
two-position one with large barrel threads.
Does anyone knows if some other company fabricates this sort of parts?
Ideally I would wish a 4-5 position rotating nosepiece large barrell, or
any other model with at least two large barrel positions. All these
lenses are infinity corrected, so I'm assuming no problem on the
distance from the scope, should I look for trouble on that as well?
Last case scenario, anyone knows of an alternative (with better price)
or second one for one of these Olympus 2 position sliding pieces?

thanks in advance

--


**********************************************************
Jose' A. Feijo', Prof.
----------------------------------------------------------
Dep. Cell Biology and Molecular Genetics
University of Maryland
0118 BioScience Research Bldg, College Park
MD 20742-5815, U.S.A.

Phone [phone] Fax [phone]
Email: [hidden email]

and/ e

Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL

tel. [phone]/19, fax [phone]
__________________________________________________________
                         
URL: http://www.cbmg.umd.edu/faculty/josfeij
or http://www.igc.gulbenkian.pt/research/unit/38

**********************************************************

[Guy Cox-2]

José,

        So you are resurrecting the old MRC 1024!  How many years ago was it that I came to IGC to give you a hand with getting that set up?  Or is this another you found in the basement in Maryland?

        I think you are making a mountain out of a molehill.  Just get a workshop to turn you up some stepped adapters so that you can use 'fat' objectives on a 'skinny' nosepiece.    The extra length won't affect optical correction since you are in 'infinity' space.  (That is assuming that the tube lens corrections are the same for both but you have that problem anyway.  In 2-photon that's unimportant.)  You may get some vignetting, and it may be a bit worse than with a proper nosepiece, though you'd get it both ways.  Again, unlikely to be important.  Cost - negligible, I think.  

                                  Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of "José A. Feijó"
Sent: Wednesday, 17 June 2015 7:51 AM
To: [hidden email]
Subject: nosepiece Olympus BX51WI two-photon mount

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I'm trying to get a Bio-rad two-photon with and Olympus BX51WI optics aup and running. I need a nosepiece for that, but the lenses I have are all large barrel. Olympus sells a 6 position rotating one, but with the old, thinner thread. And then they have a very expensive sliding two-position one with large barrel threads.
Does anyone knows if some other company fabricates this sort of parts?
Ideally I would wish a 4-5 position rotating nosepiece large barrell, or any other model with at least two large barrel positions. All these lenses are infinity corrected, so I'm assuming no problem on the distance from the scope, should I look for trouble on that as well?
Last case scenario, anyone knows of an alternative (with better price) or second one for one of these Olympus 2 position sliding pieces?

thanks in advance

--


**********************************************************
Jose' A. Feijo', Prof.
----------------------------------------------------------
Dep. Cell Biology and Molecular Genetics University of Maryland
0118 BioScience Research Bldg, College Park MD 20742-5815, U.S.A.

Phone [phone] Fax [phone]
Email: [hidden email]

and/ e

Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL

tel. [phone]/19, fax [phone] __________________________________________________________
                         
URL: http://www.cbmg.umd.edu/faculty/josfeij
or http://www.igc.gulbenkian.pt/research/unit/38

**********************************************************

[Eric Marino]

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

I might suggest ThorLabs, they have M27, M25 to RMS and vice-versa adapters…  http://www.thorlabs.com/search/thorsearch.cfm?search=rms+m25+adapter <http://www.thorlabs.com/search/thorsearch.cfm?search=rms+m25+adapter>


Eric Marino
[hidden email]

> On Jun 17, 2015, at 4:46 AM, Guy Cox <[hidden email]> wrote:
>
> José,
>
> So you are resurrecting the old MRC 1024!  How many years ago was it that I came to IGC to give you a hand with getting that set up?  Or is this another you found in the basement in Maryland?
>
> I think you are making a mountain out of a molehill.  Just get a workshop to turn you up some stepped adapters so that you can use 'fat' objectives on a 'skinny' nosepiece.    The extra length won't affect optical correction since you are in 'infinity' space.  (That is assuming that the tube lens corrections are the same for both but you have that problem anyway.  In 2-photon that's unimportant.)  You may get some vignetting, and it may be a bit worse than with a proper nosepiece, though you'd get it both ways.  Again, unlikely to be important.  Cost - negligible, I think.  
>
> Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of "José A. Feijó"
> Sent: Wednesday, 17 June 2015 7:51 AM
> To: [hidden email]
> Subject: nosepiece Olympus BX51WI two-photon mount
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I'm trying to get a Bio-rad two-photon with and Olympus BX51WI optics aup and running. I need a nosepiece for that, but the lenses I have are all large barrel. Olympus sells a 6 position rotating one, but with the old, thinner thread. And then they have a very expensive sliding two-position one with large barrel threads.
> Does anyone knows if some other company fabricates this sort of parts?
> Ideally I would wish a 4-5 position rotating nosepiece large barrell, or any other model with at least two large barrel positions. All these lenses are infinity corrected, so I'm assuming no problem on the distance from the scope, should I look for trouble on that as well?
> Last case scenario, anyone knows of an alternative (with better price) or second one for one of these Olympus 2 position sliding pieces?
>
> thanks in advance
>
> --
>
>
> **********************************************************
> Jose' A. Feijo', Prof.
> ----------------------------------------------------------
> Dep. Cell Biology and Molecular Genetics University of Maryland
> 0118 BioScience Research Bldg, College Park MD 20742-5815, U.S.A.
>
> Phone [phone] Fax [phone]
> Email: [hidden email]
>
> and/ e
>
> Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL
>
> tel. [phone]/19, fax [phone] __________________________________________________________
>
> URL: http://www.cbmg.umd.edu/faculty/josfeij
> or http://www.igc.gulbenkian.pt/research/unit/38
>
> **********************************************************