not quite confocal, but - microscope slide scanning questions
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Cromey, Douglas W - (dcromey) |
not quite confocal, but - microscope slide scanning questions
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We are trying to put together a proposal to add microscope slide scanning to our core facility. We have looked at the scanner offerings from Olympus & Leica/Aperio, I know there are others but "the powers that be" have their preferences. If you have a slide scanner in your core, would you be willing to tell me: * Approximate number of slides scanned per year (if it is an option in your core, if you could break down color brightfield vs fluorescence, that would be great). I hear stories that slide scanners are busy instruments, but I don't know how to realistically gauge the capacity of a given instrument (regardless of what the vendor says) and the actual need (we all know users that claim they need a very high percentage of an instrument's capacity, until they have to pay for the time...). * Is yours an "only core facility staff operates the instrument" service, or do you train/allow users to work on the scanner? I would think that allowing a number of different users to load a high-throughput slide scanner would reduce its overall capacity, not to mention what sloppy users can do to nice equipment. * It seems to me that if this was a "core facility staff only" operated instrument that you would need roughly a half-time employee to run the service with: accessioning of multiple projects, checking slides for cleanliness, loading the scanner, setting up the scans on each slide, managing the data after the scans have been completed, returning the slides to their owners, billing, etc. Does that sound correct? * Any other caveats that you'd like to pass along? Thanks! If you would like to respond privately, please feel free. Doug ------------------------------------------------------------------------------------------ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA office: LSN 463 email: [hidden email]<mailto:[hidden email]> voice: 520-626-2824 fax: 520-626-2097 http://microscopy.arizona.edu/learn/microscopy-imaging-resources-www Home of: "Microscopy and Imaging Resources on the WWW" UA Microscopy Alliance - http://microscopy.arizona.edu<http://microscopy.arizona.edu/> |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We have 4 slide scanners at our facility, all from 3DHistech. One can hold 250, another 150, and 2 can hold 12 slides at a time. And they are all running 80% of the time. Last week was relatively quiet week: 131 FL slides, 279 BF slides in one week A few weeks before that was pretty busy: 354 FL slides, 193 BF slides in one week. There's no electronic record of how many slides, so I can't give you accurate number of slides for the year. Slide number is one thing, but remember BF slides are much much faster to scan than FL slides in terms of time. Especially on our scanners, we have custom filters for specific Alexas and often don't use a Quad filter cube. Our machines are operated by staff only. Users drop off their slides and pick up the data once scanned. We are a big facility and have multiple staff members, but half of one staff member being solely responsible for just the scanners is about right. I would seriously consider getting one scanner for FL and another for BF; again because of the time it takes to scan FL slides vs BF slides. You can have one scan a bunch of BF slides, while another work overtime on FL slides. Also, one thing to consider is what your users tend to bring. 200 H&E slides is a lot of slides but scanning them is a breeze; it's one setting, scanner will work overnight and all is done. It's far more annoying when one user brings 2 slides with 3-channel IF staining and another brings 5 slides with 2 different IF slides, etc etc. Each set requires different set-up. While many scanners can string together different setups for different slides, it still takes time to determine the parameters. It's worked really well for us to have 4 different scanners (but essentially the same except for slide capacity), so we can load all BF slides into one and meanwhile run multiple separate sessions on other scanners for FL slides. Hope that helps. Sho Fujisawa Senior Digital Microscopist Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 417 E68th St. ZRC-1840 New York, NY 10065 646-888-2186 [hidden email] -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cromey, Douglas W - (dcromey) Sent: Wednesday, April 25, 2018 12:31 PM To: [hidden email] Subject: [EXTERNAL] not quite confocal, but - microscope slide scanning questions ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We are trying to put together a proposal to add microscope slide scanning to our core facility. We have looked at the scanner offerings from Olympus & Leica/Aperio, I know there are others but "the powers that be" have their preferences. If you have a slide scanner in your core, would you be willing to tell me: * Approximate number of slides scanned per year (if it is an option in your core, if you could break down color brightfield vs fluorescence, that would be great). I hear stories that slide scanners are busy instruments, but I don't know how to realistically gauge the capacity of a given instrument (regardless of what the vendor says) and the actual need (we all know users that claim they need a very high percentage of an instrument's capacity, until they have to pay for the time...). * Is yours an "only core facility staff operates the instrument" service, or do you train/allow users to work on the scanner? I would think that allowing a number of different users to load a high-throughput slide scanner would reduce its overall capacity, not to mention what sloppy users can do to nice equipment. * It seems to me that if this was a "core facility staff only" operated instrument that you would need roughly a half-time employee to run the service with: accessioning of multiple projects, checking slides for cleanliness, loading the scanner, setting up the scans on each slide, managing the data after the scans have been completed, returning the slides to their owners, billing, etc. Does that sound correct? * Any other caveats that you'd like to pass along? Thanks! If you would like to respond privately, please feel free. Doug ------------------------------------------------------------------------------------------ Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA office: LSN 463 email: [hidden email]<mailto:[hidden email]> voice: 520-626-2824 fax: 520-626-2097 http://microscopy.arizona.edu/learn/microscopy-imaging-resources-www Home of: "Microscopy and Imaging Resources on the WWW" UA Microscopy Alliance - http://microscopy.arizona.edu<http://microscopy.arizona.edu/> *** Only open attachments or links from trusted senders. Report phishing to [hidden email] *** ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. 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