pH probe

[Renato Mortara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello,
 
A colleague is interested in monitoring intracellular pH in live cells, in
the 5.5 - 7.5 range. In the past we used SNAFL-calcein with good results but
it is no longer available.

Any suggestions are most welcome.
 
Thanks in advance,
 
Renato Mortara
Parasitology Division
EPM-UNIFESP
São Paulo SP
www.ecb.epm.br/~ramortara
[hidden email]

[MORONE Diego RIC]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Renato,

BCECF-AM from Molecular Probes should do the trick

Diego


................................
Diego Morone
Research Center
ISTITUTO CLINICO HUMANITAS
via A. Manzoni, 113 - 20089 Rozzano - Milano, Italy
Phone: [phone] - Fax: [phone]
Email: [hidden email]
Map and directions: http://goo.gl/maps/pVWbq
www.humanitas.it/hur/cms/english/




------------------------------------------
DAI IL TUO 5x1000 ALLA RICERCA HUMANITAS.

Scegli Fondazione Humanitas per la Ricerca.

Codice fiscale 97408620157 (Ricerca Scientifica e Università)

http://www.iomerito.it
------------------------------------------
-----Original Message-----
From: Renato [mailto:[hidden email]]
Sent: mercoledì 24 aprile 2013 02:04
Subject: pH probe

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello,

A colleague is interested in monitoring intracellular pH in live cells, in the 5.5 - 7.5 range. In the past we used SNAFL-calcein with good results but it is no longer available.

Any suggestions are most welcome.

Thanks in advance,

Renato Mortara
Parasitology Division
EPM-UNIFESP
São Paulo SP
www.ecb.epm.br/~ramortara
[hidden email]