pH sensitive dye

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John Garner John Garner
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pH sensitive dye

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Hi Everyone,
 
I am searching for a good pH sensitive dye to detect very low pH values (roughly 2-6).  I have found CL-NERF has been used for this purpose before, but molecular probes (now Invitrogen) no longer manufactures this dye and I can not find any other sources of it.  Does anyone happen to have any of this dye or know where it can be procured?  Alternatively, are there any good suggestions for any other dye that can detect at these pH values?
 
Thanks,
 
John Garner
Formulations Specialist
Akina Inc.
1291 Cumberland Avenue
West Lafayette, IN 47906
Tel: (765) 464-0501 x6
Fax: (765) 464-0820
[hidden email]
Ignatius, Mike Ignatius, Mike
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Re: pH sensitive dye ***Vendor Response***

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi John,
 
We would suggest trying LysoSensor Yellow/Blue DND-160 (L7545).  It has a pKa of 4.2 and is useful in the range of 3.5 to 6.0.  It actually becomes more fluorescent with increasing acidity.
 
Regards,
 
Mike Ignatius, Ph.D.
Molecular Probes labeling and detection technologies
INVITROGEN | 29851 Willow Creek Road, Eugene, OR 97402-9132
Tel: 541-335-0414 | [hidden email]
 
With help from Magnus Persmark who researched this response.
 


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Garner
Sent: Thursday, September 27, 2007 11:46 AM
To: [hidden email]
Subject: pH sensitive dye

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Everyone,
 
I am searching for a good pH sensitive dye to detect very low pH values (roughly 2-6).  I have found CL-NERF has been used for this purpose before, but molecular probes (now Invitrogen) no longer manufactures this dye and I can not find any other sources of it.  Does anyone happen to have any of this dye or know where it can be procured?  Alternatively, are there any good suggestions for any other dye that can detect at these pH values?
 
Thanks,
 
John Garner
Formulations Specialist
Akina Inc.
1291 Cumberland Avenue
West Lafayette, IN 47906
Tel: (765) 464-0501 x6
Fax: (765) 464-0820
[hidden email]