perfusion chamber

[Renato A. Mortara]

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Hi,

I am trying to image ex-vivo tissue sections on a Leica SP5 confocal. So far
we managed to image on glass-bottom dishes for a few hours but would need to
perfuse the sections in order to extend the observations.

Any suggestions ?

Many thanks !

Renato Mortara


Renato Arruda Mortara
Parasitology Division
Escola Paulista de Medicina – UNIFESP
Rua Botucatu, 862 6º andar
04023-062
São Paulo SP
Brazil
Phone: [phone]
www.ecb.epm.br/~ramortara

[Craig Brideau]

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Warner Instruments makes tissue slice chambers for perfusion that work
fairly well. We use them for everything from live sections to spinal cords.
www.warneronline.com
Tissue Slice Chamber RC-27L

No commercial interest, just use them around the lab.

Craig Brideau


On Thu, Sep 4, 2014 at 5:51 AM, Renato Mortara <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I am trying to image ex-vivo tissue sections on a Leica SP5 confocal. So
> far
> we managed to image on glass-bottom dishes for a few hours but would need
> to
> perfuse the sections in order to extend the observations.
>
> Any suggestions ?
>
> Many thanks !
>
> Renato Mortara
>
>
> Renato Arruda Mortara
> Parasitology Division
> Escola Paulista de Medicina – UNIFESP
> Rua Botucatu, 862 6º andar
> 04023-062
> São Paulo SP
> Brazil
> Phone: [phone]
> www.ecb.epm.br/~ramortara
>

[Renato A. Mortara]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks everyone for the helpful suggestions !

Best

Renato

-----Mensagem original-----
De: Confocal Microscopy List [mailto:[hidden email]] Em nome de Craig Brideau
Enviada em: quinta-feira, 4 de setembro de 2014 14:36
Para: [hidden email]
Assunto: Re: perfusion chamber

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Warner Instruments makes tissue slice chambers for perfusion that work fairly well. We use them for everything from live sections to spinal cords.
www.warneronline.com
Tissue Slice Chamber RC-27L

No commercial interest, just use them around the lab.

Craig Brideau


On Thu, Sep 4, 2014 at 5:51 AM, Renato Mortara <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I am trying to image ex-vivo tissue sections on a Leica SP5 confocal.
> So far we managed to image on glass-bottom dishes for a few hours but
> would need to perfuse the sections in order to extend the
> observations.
>
> Any suggestions ?
>
> Many thanks !
>
> Renato Mortara
>
>
> Renato Arruda Mortara
> Parasitology Division
> Escola Paulista de Medicina – UNIFESP
> Rua Botucatu, 862 6º andar
> 04023-062
> São Paulo SP
> Brazil
> Phone: [phone]
> www.ecb.epm.br/~ramortara
>