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Hallo,
I am about to start a project on 3D tissue structure, mostly in Arabidopsis
leaf and apical meristems.
in general, the tissue will be fixed, cell walls stained using modified
periodic acid - chiff (with Propidium iodide) - similar to the protocol by
Wytus et al, Plant Methods. 2010; 6: 17.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909956/
If possible, I would like to avoid using Hoyer's solution/chloralhydrate for
tissue clearing.
If you tried the ScaleA2 clearing medium on plant tissue, I would love to
hear if/how well it worked.
Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, et al. Scale: a
chemical approach for fluorescence imaging and reconstruction of transparent
Smouse brain. Nat Neurosci. 2011;14:1481-8
Thank you!
Stan Vitha
Microscopy and Imaging Center
Texas A&M University
Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, et al. Scale: a
chemical approach for fluorescence imaging and reconstruction of transparent
Smouse brain. Nat Neurosci. 2011;14:1481-8
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